High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles
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High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles. / Moberg, Marcus; Apró, William; Cervenka, Igor; Ekblom, Björn; van Hall, Gerrit; Holmberg, Hans Christer; Ruas, Jorge L.; Blomstrand, Eva.
In: Scientific Reports, Vol. 11, No. 1, 6453, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles
AU - Moberg, Marcus
AU - Apró, William
AU - Cervenka, Igor
AU - Ekblom, Björn
AU - van Hall, Gerrit
AU - Holmberg, Hans Christer
AU - Ruas, Jorge L.
AU - Blomstrand, Eva
N1 - Publisher Copyright: © 2021, The Author(s).
PY - 2021
Y1 - 2021
N2 - This study examined acute molecular responses to concurrent exercise involving different muscles. Eight men participated in a randomized crossover-trial with two sessions, one where they performed interval cycling followed by upper body resistance exercise (ER-Arm), and one with upper body resistance exercise only (R-Arm). Biopsies were taken from the triceps prior to and immediately, 90- and 180-min following exercise. Immediately after resistance exercise, the elevation in S6K1 activity was smaller and the 4E-BP1:eIF4E interaction greater in ER-Arm, but this acute attenuation disappeared during recovery. The protein synthetic rate in triceps was greater following exercise than at rest, with no difference between trials. The level of PGC-1α1 mRNA increased to greater extent in ER-Arm than R-Arm after 90 min of recovery, as was PGC-1α4 mRNA after both 90 and 180 min. Levels of MuRF-1 mRNA was unchanged in R-Arm, but elevated during recovery in ER-Arm, whereas MAFbx mRNA levels increased slightly in both trials. RNA sequencing in a subgroup of subjects revealed 862 differently expressed genes with ER-Arm versus R-Arm during recovery. These findings suggest that leg cycling prior to arm resistance exercise causes systemic changes that potentiate induction of specific genes in the triceps, without compromising the anabolic response.
AB - This study examined acute molecular responses to concurrent exercise involving different muscles. Eight men participated in a randomized crossover-trial with two sessions, one where they performed interval cycling followed by upper body resistance exercise (ER-Arm), and one with upper body resistance exercise only (R-Arm). Biopsies were taken from the triceps prior to and immediately, 90- and 180-min following exercise. Immediately after resistance exercise, the elevation in S6K1 activity was smaller and the 4E-BP1:eIF4E interaction greater in ER-Arm, but this acute attenuation disappeared during recovery. The protein synthetic rate in triceps was greater following exercise than at rest, with no difference between trials. The level of PGC-1α1 mRNA increased to greater extent in ER-Arm than R-Arm after 90 min of recovery, as was PGC-1α4 mRNA after both 90 and 180 min. Levels of MuRF-1 mRNA was unchanged in R-Arm, but elevated during recovery in ER-Arm, whereas MAFbx mRNA levels increased slightly in both trials. RNA sequencing in a subgroup of subjects revealed 862 differently expressed genes with ER-Arm versus R-Arm during recovery. These findings suggest that leg cycling prior to arm resistance exercise causes systemic changes that potentiate induction of specific genes in the triceps, without compromising the anabolic response.
UR - http://www.scopus.com/inward/record.url?scp=85102873780&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-85733-1
DO - 10.1038/s41598-021-85733-1
M3 - Journal article
C2 - 33742064
AN - SCOPUS:85102873780
VL - 11
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 6453
ER -
ID: 281709952