Glycoproteins of mouse vaginal epithelium: differential expression related to estrous cyclicity.
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Glycoproteins of mouse vaginal epithelium: differential expression related to estrous cyclicity. / Horvat, B; Multhaupt, H A; Damjanov, I.
In: Journal of Histochemistry and Cytochemistry, Vol. 41, No. 9, 1993, p. 1351-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Glycoproteins of mouse vaginal epithelium: differential expression related to estrous cyclicity.
AU - Horvat, B
AU - Multhaupt, H A
AU - Damjanov, I
N1 - Keywords: Animals; Electrophoresis, Polyacrylamide Gel; Epithelium; Estrus; Female; Glycoproteins; Histocytochemistry; Lectins; Mice; Microscopy, Electron; Vagina
PY - 1993
Y1 - 1993
N2 - We used lectin overlay blotting and SDS-PAGE to analyze the estrous cycle-specific expression of mouse vaginal epithelial glycoproteins. Seven lectins chosen for their differential carbohydrate-binding specificity revealed 15 glycoproteins that showed cycle-related expression. Each lectin had a unique binding pattern different from the patterns revealed by other lectins. However, several estrous cycle phase-specific glycoproteins reacted with more than one lectin. The most prominent of these glycoproteins (M(r) 92-95 KD) was weakly expressed in late diestrus and fully expressed only in proestrus, coincident with the transformation of two superficial layers of vaginal squamous epithelium into mucinous cuboidal cells. Electron microscopic lectin histochemistry revealed the glycoproteins in the mucinous granules of surface cuboidal cells and in the lumen of the vagina. Our results illustrate the complexity of glycoconjugate synthesis in mouse vagina and reveal the distinct cycle-specific patterns of individual glycoprotein expression. These cyclic glycoproteins could serve as vaginal biochemical markers for the specific phases of the estrous cycle.
AB - We used lectin overlay blotting and SDS-PAGE to analyze the estrous cycle-specific expression of mouse vaginal epithelial glycoproteins. Seven lectins chosen for their differential carbohydrate-binding specificity revealed 15 glycoproteins that showed cycle-related expression. Each lectin had a unique binding pattern different from the patterns revealed by other lectins. However, several estrous cycle phase-specific glycoproteins reacted with more than one lectin. The most prominent of these glycoproteins (M(r) 92-95 KD) was weakly expressed in late diestrus and fully expressed only in proestrus, coincident with the transformation of two superficial layers of vaginal squamous epithelium into mucinous cuboidal cells. Electron microscopic lectin histochemistry revealed the glycoproteins in the mucinous granules of surface cuboidal cells and in the lumen of the vagina. Our results illustrate the complexity of glycoconjugate synthesis in mouse vagina and reveal the distinct cycle-specific patterns of individual glycoprotein expression. These cyclic glycoproteins could serve as vaginal biochemical markers for the specific phases of the estrous cycle.
M3 - Journal article
C2 - 8354876
VL - 41
SP - 1351
EP - 1357
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 9
ER -
ID: 5240345