Glutaraldehyde Cross-Linking of TendonMechanical Effects at the Level of the Tendon Fascicle and Fibril
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Glutaraldehyde Cross-Linking of TendonMechanical Effects at the Level of the Tendon Fascicle and Fibril. / Hansen, P.; Svensson, R.B.; Aagaard, P.; Trappe, T.; Haraldsson, B.T.; Kjaer, M.; Magnusson, P.; Hassenkam, Tue.
In: Connective Tissue Research, Vol. 50, No. 4, 2009, p. 211-222.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Glutaraldehyde Cross-Linking of TendonMechanical Effects at the Level of the Tendon Fascicle and Fibril
AU - Hansen, P.
AU - Svensson, R.B.
AU - Aagaard, P.
AU - Trappe, T.
AU - Haraldsson, B.T.
AU - Kjaer, M.
AU - Magnusson, P.
AU - Hassenkam, Tue
N1 - Times Cited: 0ArticleEnglishMagnusson, PBispebjerg Hosp, Inst Sports Med Copenhagen, Bispebjerg Bakke 23, DK-2400 Copenhagen, DenmarkCited References Count: 44475VKTAYLOR & FRANCIS LTD4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLANDABINGDON
PY - 2009
Y1 - 2009
N2 - Conclusive insight into the microscopic principles that govern the strength of tendon and related connective tissues is lacking and the importance of collagen cross-linking has not been firmly established. The combined application of whole-tissue mechanical testing and atomic force spectroscopy allowed for a detailed characterization of the effect of cross-linking in rat-tail tendon. The cross-link inducing agent glutaraldehyde augmented the tensile strength of tendon fascicles. Stress at failure increased from 8 MPa to 39 MPa. The mechanical effects of glutaraldehyde at the tendon fibril level were examined by atomic force microscopy. Peak forces increased from 1379 to 2622 pN while an extended Hertz fit of force-indentation data showed a 24 fold increase in Young's modulus on indentation. The effect of glutaraldehyde cross-linking on the tensile properties of a single collagen fibril was investigated by a novel methodology based on atomic force spectroscopy. The Young's modulus of a secluded fibril increased from 407 MPa to 1.1 GPa with glutaraldehyde treatment. Collectively, the findings indicate that cross-linking at the level of the collagen fibril is of key importance for the mechanical strength of tendon tissue. However, when comparing the effects at the level of the tendon fascicle and fibril, respectively, further questions are prompted regarding the pathways of force through the tendon microstructure as fibril strength seems to surpass that of the tendon fascicle Udgivelsesdato: 2009
AB - Conclusive insight into the microscopic principles that govern the strength of tendon and related connective tissues is lacking and the importance of collagen cross-linking has not been firmly established. The combined application of whole-tissue mechanical testing and atomic force spectroscopy allowed for a detailed characterization of the effect of cross-linking in rat-tail tendon. The cross-link inducing agent glutaraldehyde augmented the tensile strength of tendon fascicles. Stress at failure increased from 8 MPa to 39 MPa. The mechanical effects of glutaraldehyde at the tendon fibril level were examined by atomic force microscopy. Peak forces increased from 1379 to 2622 pN while an extended Hertz fit of force-indentation data showed a 24 fold increase in Young's modulus on indentation. The effect of glutaraldehyde cross-linking on the tensile properties of a single collagen fibril was investigated by a novel methodology based on atomic force spectroscopy. The Young's modulus of a secluded fibril increased from 407 MPa to 1.1 GPa with glutaraldehyde treatment. Collectively, the findings indicate that cross-linking at the level of the collagen fibril is of key importance for the mechanical strength of tendon tissue. However, when comparing the effects at the level of the tendon fascicle and fibril, respectively, further questions are prompted regarding the pathways of force through the tendon microstructure as fibril strength seems to surpass that of the tendon fascicle Udgivelsesdato: 2009
M3 - Journal article
VL - 50
SP - 211
EP - 222
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 4
ER -
ID: 20656285