Extended studies on the effect of glutamate antagonists on ischemic CA-1 damage

Research output: Contribution to journalJournal articleResearchpeer-review

  • Nils Henrik Diemer
  • T Balchen
  • T Bruhn
  • Thomas Christensen
  • I Vanicky
  • M Nielsen
  • F F Johansen
Glutamate receptors are numerous on the ischemia vulnerable CA-1 pyramidal cells. Postischemic use of the AMPA antagonist NBQX has shown up to 80% protection against cell death. Three aspects of this were studied: In the first study, male Wistar rats were given NBQX (30 mg/kg x 3) either 20 hours or immediately (0 h) before 12 min of 4-vessel occlusion with hypotension. After six days of reperfusion comparison with an untreated group showed almost full protection in the 0 h group (4% cell loss, p <0.001) but only slight protection in the 20 h group (62% cell loss, p <0.05). After 12 min of ischemia in the present model, eosinophilic CA-1 cells are seen from day 2 on. Since there could be a late, deleterious calcium influx via NMDA receptors, one group of ischemic rats was given MK-801 (5 mg/kg i.p.) 24 hours after ischemia. However, quantitation 6 days later of remaining CA-1 cells showed no protection. In the third study referred here, two groups of ischemic rats were given NBQX (30 mg/kg x 3) immediately after ischemia. The groups survive for six and 21 days, respectively. Counting of CA-1 pyramidal cells showed an equal, significant protection in both groups (approx 20% cell loss).
Original languageEnglish
Book seriesActa Neurochirurgica Supplement
Volume66
Pages (from-to)73-5
Number of pages3
ISSN0065-1419
Publication statusPublished - 1996

    Research areas

  • Animals, Brain Damage, Chronic, Brain Ischemia, Brain Mapping, Dizocilpine Maleate, Dose-Response Relationship, Drug, Drug Administration Schedule, Excitatory Amino Acid Antagonists, Glutamic Acid, Hippocampus, Male, Neurons, Premedication, Quinoxalines, Rats, Rats, Wistar, Receptors, AMPA, Receptors, N-Methyl-D-Aspartate

ID: 45392520