Elk3 from hamster--a ternary complex factor with strong transcriptional repressor activity
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Elk3 from hamster--a ternary complex factor with strong transcriptional repressor activity. / Hjortoe, Gertrud Malene; Weilguny, Dietmar; Willumsen, Berthe Marie.
In: D N A and Cell Biology, Vol. 24, No. 1, 01.2005, p. 35-42.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Elk3 from hamster--a ternary complex factor with strong transcriptional repressor activity
AU - Hjortoe, Gertrud Malene
AU - Weilguny, Dietmar
AU - Willumsen, Berthe Marie
PY - 2005/1
Y1 - 2005/1
N2 - Elk3 belongs to the Ets family of transcription factors, which are regulated by the Ras/mitogen-activated protein kinase-signaling pathway. In the absence of Ras, this protein is a strong inhibitor of transcription and may be directly involved in regulation of growth by downregulating the transcription of genes that are activated during entry into G1. We have isolated the Cricetulus griseus Elk3 gene from the Chinese hamster ovary (CHO) cell line and investigated the transcriptional potential of this factor. Transient transfections revealed that, in addition to its regulation of the c-fos promoter, Elk3 from CHO cells seems to inhibit other promoters controlling expression of proteins involved in G1/S phase progression; Cyclin D1 and DHFR. As has been described for the Elk3 homologs Net (Mouse) and Sap-2 (Human), the results of the present study further indicate that hamster Elk3 is a target of the Ras-Raf-MAPK pathway, and cotransfections with constitutively active H-ras relieves its negative transcriptional activity. No cells stably expressing exogenous Elk3 could be obtained, possibly due to an unspecified toxic or growth retarding effect. These findings support a possible role for Elk3 in growth regulation and reveal a high degree of homology for this protein across species.
AB - Elk3 belongs to the Ets family of transcription factors, which are regulated by the Ras/mitogen-activated protein kinase-signaling pathway. In the absence of Ras, this protein is a strong inhibitor of transcription and may be directly involved in regulation of growth by downregulating the transcription of genes that are activated during entry into G1. We have isolated the Cricetulus griseus Elk3 gene from the Chinese hamster ovary (CHO) cell line and investigated the transcriptional potential of this factor. Transient transfections revealed that, in addition to its regulation of the c-fos promoter, Elk3 from CHO cells seems to inhibit other promoters controlling expression of proteins involved in G1/S phase progression; Cyclin D1 and DHFR. As has been described for the Elk3 homologs Net (Mouse) and Sap-2 (Human), the results of the present study further indicate that hamster Elk3 is a target of the Ras-Raf-MAPK pathway, and cotransfections with constitutively active H-ras relieves its negative transcriptional activity. No cells stably expressing exogenous Elk3 could be obtained, possibly due to an unspecified toxic or growth retarding effect. These findings support a possible role for Elk3 in growth regulation and reveal a high degree of homology for this protein across species.
KW - Amino Acid Sequence
KW - Animals
KW - CHO Cells
KW - Cell Cycle
KW - Cricetinae
KW - Cricetulus
KW - Cyclin D1
KW - DNA-Binding Proteins
KW - Down-Regulation
KW - Genes, Reporter
KW - Genes, fos
KW - Genes, ras
KW - Molecular Sequence Data
KW - Promoter Regions, Genetic
KW - Protein Structure, Tertiary
KW - Repressor Proteins
KW - Sequence Alignment
KW - Tetrahydrofolate Dehydrogenase
KW - Transcription Factors
KW - Transcription, Genetic
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1089/dna.2005.24.35
DO - 10.1089/dna.2005.24.35
M3 - Journal article
C2 - 15684718
VL - 24
SP - 35
EP - 42
JO - DNA and Cell Biology
JF - DNA and Cell Biology
SN - 1044-5498
IS - 1
ER -
ID: 182199456