Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands.

Research output: Contribution to journal › Journal article › Research › peer-review

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Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands. / Grönberg, Malin; Tsolakis, Apostolos V; Magnusson, Linda; Janson, Eva T; Saras, Jan.

In: Journal of Histochemistry and Cytochemistry, Vol. 56, No. 9, 2008, p. 793-801.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Grönberg, M, Tsolakis, AV, Magnusson, L, Janson, ET & Saras, J 2008, 'Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands.', Journal of Histochemistry and Cytochemistry, vol. 56, no. 9, pp. 793-801. https://doi.org/10.1369/jhc.2008.951145

APA

Grönberg, M., Tsolakis, A. V., Magnusson, L., Janson, E. T., & Saras, J. (2008). Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands. Journal of Histochemistry and Cytochemistry, 56(9), 793-801. https://doi.org/10.1369/jhc.2008.951145

Vancouver

Grönberg M, Tsolakis AV, Magnusson L, Janson ET, Saras J. Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands. Journal of Histochemistry and Cytochemistry. 2008;56(9):793-801. https://doi.org/10.1369/jhc.2008.951145

Author

Grönberg, Malin ; Tsolakis, Apostolos V ; Magnusson, Linda ; Janson, Eva T ; Saras, Jan. / Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands. In: Journal of Histochemistry and Cytochemistry. 2008 ; Vol. 56, No. 9. pp. 793-801.

Bibtex

@article{7d6a3e40acd111ddb538000ea68e967b,
title = "Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands.",
abstract = "Obestatin and ghrelin are two peptides derived from the same prohormone. It is well established that ghrelin is produced by endocrine cells in the gastric mucosa. However, the distribution of human obestatin immunoreactive cells is not thoroughly characterized. A polyclonal antibody that specifically recognizes human obestatin was produced. Using this antibody and a commercial antibody vs ghrelin, the distribution of obestatin and ghrelin immunoreactive cells was determined in a panel of human tissues using immunohistochemistry. The two peptides were detected in the mucosa of the gastrointestinal tract, from cardia to ileum, and in the pancreatic islets. Interestingly, epithelial cells in the ducts of mammary glands showed distinct immunoreactivity for both ghrelin and obestatin. By double immunofluorescence microscopy, it was shown that all detected cells were immunoreactive for both peptides. Furthermore, the subcellular localization of obestatin and ghrelin was essentially identical, indicating that obestatin and ghrelin are stored in the same secretory vesicles.",
author = "Malin Gr{\"o}nberg and Tsolakis, {Apostolos V} and Linda Magnusson and Janson, {Eva T} and Jan Saras",
note = "Keywords: Gastrointestinal Tract; Ghrelin; Humans; Immunohistochemistry; Mammary Glands, Human; Organ Specificity; Pancreas",
year = "2008",
doi = "10.1369/jhc.2008.951145",
language = "English",
volume = "56",
pages = "793--801",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "SAGE Publications",
number = "9",

}

RIS

TY - JOUR

T1 - Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands.

AU - Grönberg, Malin

AU - Tsolakis, Apostolos V

AU - Magnusson, Linda

AU - Janson, Eva T

AU - Saras, Jan

N1 - Keywords: Gastrointestinal Tract; Ghrelin; Humans; Immunohistochemistry; Mammary Glands, Human; Organ Specificity; Pancreas

PY - 2008

Y1 - 2008

N2 - Obestatin and ghrelin are two peptides derived from the same prohormone. It is well established that ghrelin is produced by endocrine cells in the gastric mucosa. However, the distribution of human obestatin immunoreactive cells is not thoroughly characterized. A polyclonal antibody that specifically recognizes human obestatin was produced. Using this antibody and a commercial antibody vs ghrelin, the distribution of obestatin and ghrelin immunoreactive cells was determined in a panel of human tissues using immunohistochemistry. The two peptides were detected in the mucosa of the gastrointestinal tract, from cardia to ileum, and in the pancreatic islets. Interestingly, epithelial cells in the ducts of mammary glands showed distinct immunoreactivity for both ghrelin and obestatin. By double immunofluorescence microscopy, it was shown that all detected cells were immunoreactive for both peptides. Furthermore, the subcellular localization of obestatin and ghrelin was essentially identical, indicating that obestatin and ghrelin are stored in the same secretory vesicles.

AB - Obestatin and ghrelin are two peptides derived from the same prohormone. It is well established that ghrelin is produced by endocrine cells in the gastric mucosa. However, the distribution of human obestatin immunoreactive cells is not thoroughly characterized. A polyclonal antibody that specifically recognizes human obestatin was produced. Using this antibody and a commercial antibody vs ghrelin, the distribution of obestatin and ghrelin immunoreactive cells was determined in a panel of human tissues using immunohistochemistry. The two peptides were detected in the mucosa of the gastrointestinal tract, from cardia to ileum, and in the pancreatic islets. Interestingly, epithelial cells in the ducts of mammary glands showed distinct immunoreactivity for both ghrelin and obestatin. By double immunofluorescence microscopy, it was shown that all detected cells were immunoreactive for both peptides. Furthermore, the subcellular localization of obestatin and ghrelin was essentially identical, indicating that obestatin and ghrelin are stored in the same secretory vesicles.

U2 - 10.1369/jhc.2008.951145

DO - 10.1369/jhc.2008.951145

M3 - Journal article

C2 - 18474938

VL - 56

SP - 793

EP - 801

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 9

ER -

ID: 8465525