Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
Original language | English |
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Title of host publication | P-Type ATPases : Methods and Protocols |
Editors | Maike Bublitz |
Number of pages | 8 |
Volume | 1377 |
Publisher | Springer |
Publication date | 2016 |
Pages | 413-420 |
Chapter | 37 |
ISBN (Print) | 978-1-4939-3178-1 |
ISBN (Electronic) | 978-1-4939-3179-8 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1377 |
ISSN | 1064-3745 |
ID: 174864931