Clonal evolution demonstrated by flow cytometric DNA analysis of a human colonic carcinoma grown in nude mice
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Clonal evolution demonstrated by flow cytometric DNA analysis of a human colonic carcinoma grown in nude mice. / Vindeløv, L L; Spang-Thomsen, M; Visfeldt, J; Povlsen, C O; Jensen, G; Nissen, N I.
In: Experimental Cell Biology, Vol. 50, No. 4, 1982, p. 216-21.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Clonal evolution demonstrated by flow cytometric DNA analysis of a human colonic carcinoma grown in nude mice
AU - Vindeløv, L L
AU - Spang-Thomsen, M
AU - Visfeldt, J
AU - Povlsen, C O
AU - Jensen, G
AU - Nissen, N I
N1 - Keywords: Animals; Cell Division; Clone Cells; Colonic Neoplasms; DNA, Neoplasm; Flow Cytometry; Humans; Mice; Mice, Nude; Neoplasm Transplantation; Pedigree
PY - 1982
Y1 - 1982
N2 - A spontaneous change in DNA content of a human colonic carcinoma grown in nude mice was observed fortuitously. The tumor initially had a G1 cell DNA content of 1.3 times that of normal cells. Flow cytometric DNA analysis showed in transplant generation 56 the appearance of a new subpopulation which in three passages completely overgrew the original population. The DNA content of the new subpopulation was twice that of the original population. The observation supports the hypothesis of clonal evolution of tumor cell populations. The growth rates of the tumor before and after the change showed no significant difference (p greater than 0.05). Cell kinetic factors, therefore, offer no obvious explanation of how the overgrowth took place. It is not known whether the original population disappeared completely or survived as a small population below the detection limit. The heterogeneity created by clonal evolution of a tumor would be less pronounced if old subpopulations often become extinct as new ones emerge. Heterogeneity of human tumors is of clinical importance because the individual subpopulations may have different sensitivity patterns to antineoplastic drugs.
AB - A spontaneous change in DNA content of a human colonic carcinoma grown in nude mice was observed fortuitously. The tumor initially had a G1 cell DNA content of 1.3 times that of normal cells. Flow cytometric DNA analysis showed in transplant generation 56 the appearance of a new subpopulation which in three passages completely overgrew the original population. The DNA content of the new subpopulation was twice that of the original population. The observation supports the hypothesis of clonal evolution of tumor cell populations. The growth rates of the tumor before and after the change showed no significant difference (p greater than 0.05). Cell kinetic factors, therefore, offer no obvious explanation of how the overgrowth took place. It is not known whether the original population disappeared completely or survived as a small population below the detection limit. The heterogeneity created by clonal evolution of a tumor would be less pronounced if old subpopulations often become extinct as new ones emerge. Heterogeneity of human tumors is of clinical importance because the individual subpopulations may have different sensitivity patterns to antineoplastic drugs.
M3 - Journal article
C2 - 7117665
VL - 50
SP - 216
EP - 221
JO - Experimental Cell Biology
JF - Experimental Cell Biology
SN - 0304-3568
IS - 4
ER -
ID: 12872589