Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells.
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Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells. / Czuchra, Aleksandra; Wu, Xunwei; Meyer, Hannelore; van Hengel, Jolanda; Schroeder, Timm; Geffers, Robert; Rottner, Klemens; Brakebusch, Cord.
In: Molecular Biology of the Cell, Vol. 16, No. 10, 2005, p. 4473-84.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells.
AU - Czuchra, Aleksandra
AU - Wu, Xunwei
AU - Meyer, Hannelore
AU - van Hengel, Jolanda
AU - Schroeder, Timm
AU - Geffers, Robert
AU - Rottner, Klemens
AU - Brakebusch, Cord
N1 - Keywords: Cell Adhesion; Cell Line; Cell Movement; Cell Polarity; Cell Shape; Fibroblasts; Golgi Apparatus; Humans; Mitosis; Pseudopodia; cdc42 GTP-Binding Protein; rac1 GTP-Binding Protein
PY - 2005
Y1 - 2005
N2 - Cdc42 is a small GTPase involved in the regulation of the cytoskeleton and cell polarity. To test whether Cdc42 has an essential role in the formation of filopodia or directed cell migration, we generated Cdc42-deficient fibroblastoid cells by conditional gene inactivation. We report here that loss of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the Golgi apparatus into the direction of migration was decreased. However, expression of dominant negative Cdc42 in Cdc42-null cells resulted in strongly reduced directed migration, severely reduced single cell directionality, and complete loss of Golgi polarization and of directionality of protrusion formation toward the wound, as well as membrane blebbing. Thus, our data show that besides Cdc42 additional GTPases of the Rho-family, which share GEFs with Cdc42, are involved in the establishment and maintenance of cell polarity during directed migration.
AB - Cdc42 is a small GTPase involved in the regulation of the cytoskeleton and cell polarity. To test whether Cdc42 has an essential role in the formation of filopodia or directed cell migration, we generated Cdc42-deficient fibroblastoid cells by conditional gene inactivation. We report here that loss of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the Golgi apparatus into the direction of migration was decreased. However, expression of dominant negative Cdc42 in Cdc42-null cells resulted in strongly reduced directed migration, severely reduced single cell directionality, and complete loss of Golgi polarization and of directionality of protrusion formation toward the wound, as well as membrane blebbing. Thus, our data show that besides Cdc42 additional GTPases of the Rho-family, which share GEFs with Cdc42, are involved in the establishment and maintenance of cell polarity during directed migration.
U2 - 10.1091/mbc.E05-01-0061
DO - 10.1091/mbc.E05-01-0061
M3 - Journal article
C2 - 16014609
VL - 16
SP - 4473
EP - 4484
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 10
ER -
ID: 5141146