Brain response to traumatic brain injury in wild-type and interleukin-6 knockout mice: a microarray analysis
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Traumatic injury to the brain is one of the leading causes of injury-related death or disability. Brain response to injury is orchestrated by cytokines, such as interleukin (IL)-6, but the full repertoire of responses involved is not well known. We here report the results obtained with microarrays in wild-type and IL-6 knockout mice subjected to a cryolesion of the somatosensorial cortex and killed at 0, 1, 4, 8 and 16 days post-lesion. Overall gene expression was analyzed by using Affymetrix genechips/oligonucleotide arrays with approximately 12,400 probe sets corresponding to approximately 10,000 different murine genes (MG_U74Av2). A robust, conventional statistical method (two-way anova) was employed to select the genes significantly affected. An orderly pattern of gene responses was clearly detected, with genes being up- or down-regulated at specific timings consistent with the processes involved in the initial tissue injury and later regeneration of the parenchyma. IL-6 deficiency showed a dramatic effect in the expression of many genes, especially in the 1 day post-lesion timing, which presumably underlies the poor capacity of IL-6 knockout mice to cope with brain damage. The results highlight the importance of IL-6 controlling the response of the brain to injury as well as the suitability of microarrays for identifying specific targets worthy of further study.
Original language | English |
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Journal | Journal of Neurochemistry |
Volume | 92 |
Issue number | 2 |
Pages (from-to) | 417-32 |
Number of pages | 15 |
ISSN | 0022-3042 |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Analysis of Variance; Animals; Brain; Brain Injuries; Cluster Analysis; Disease Progression; Down-Regulation; Gene Expression Profiling; Interleukin-6; Mice; Mice, Inbred C57BL; Mice, Knockout; Oligonucleotide Array Sequence Analysis; Up-Regulation
ID: 13620453