An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline. / Frøkjær-Jensen, Christian; Jain, Nimit; Hansen, Loren; Davis, M Wayne; Li, Yongbin; Zhao, Di; Rebora, Karine; Millet, Jonathan R R M; Liu, Xiao; Kim, Stuart K; Dupuy, Denis; Jorgensen, Erik M; Fire, Andrew Z.

In: Cell, Vol. 166, No. 2, 14.07.2016, p. 343-357.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Frøkjær-Jensen, C, Jain, N, Hansen, L, Davis, MW, Li, Y, Zhao, D, Rebora, K, Millet, JRRM, Liu, X, Kim, SK, Dupuy, D, Jorgensen, EM & Fire, AZ 2016, 'An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline', Cell, vol. 166, no. 2, pp. 343-357. https://doi.org/10.1016/j.cell.2016.05.072

APA

Frøkjær-Jensen, C., Jain, N., Hansen, L., Davis, M. W., Li, Y., Zhao, D., Rebora, K., Millet, JR. R. M., Liu, X., Kim, S. K., Dupuy, D., Jorgensen, E. M., & Fire, A. Z. (2016). An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline. Cell, 166(2), 343-357. https://doi.org/10.1016/j.cell.2016.05.072

Vancouver

Frøkjær-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D et al. An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline. Cell. 2016 Jul 14;166(2):343-357. https://doi.org/10.1016/j.cell.2016.05.072

Author

Frøkjær-Jensen, Christian ; Jain, Nimit ; Hansen, Loren ; Davis, M Wayne ; Li, Yongbin ; Zhao, Di ; Rebora, Karine ; Millet, Jonathan R R M ; Liu, Xiao ; Kim, Stuart K ; Dupuy, Denis ; Jorgensen, Erik M ; Fire, Andrew Z. / An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline. In: Cell. 2016 ; Vol. 166, No. 2. pp. 343-357.

Bibtex

@article{41468d36e6c2485a97ba672061a59bc7,
title = "An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline",
abstract = "Cells benefit from silencing foreign genetic elements but must simultaneously avoid inactivating endogenous genes. Although chromatin modifications and RNAs contribute to maintenance of silenced states, the establishment of silenced regions will inevitably reflect underlying DNA sequence and/or structure. Here, we demonstrate that a pervasive non-coding DNA feature in Caenorhabditis elegans, characterized by 10-base pair periodic An/Tn-clusters (PATCs), can license transgenes for germline expression within repressive chromatin domains. Transgenes containing natural or synthetic PATCs are resistant to position effect variegation and stochastic silencing in the germline. Among endogenous genes, intron length and PATC-character undergo dramatic changes as orthologs move from active to repressive chromatin over evolutionary time, indicating a dynamic character to the An/Tn periodicity. We propose that PATCs form the basis of a cellular immune system, identifying certain endogenous genes in heterochromatic contexts as privileged while foreign DNA can be suppressed with no requirement for a cellular memory of prior exposure.",
author = "Christian Fr{\o}kj{\ae}r-Jensen and Nimit Jain and Loren Hansen and Davis, {M Wayne} and Yongbin Li and Di Zhao and Karine Rebora and Millet, {Jonathan R R M} and Xiao Liu and Kim, {Stuart K} and Denis Dupuy and Jorgensen, {Erik M} and Fire, {Andrew Z.}",
year = "2016",
month = jul,
day = "14",
doi = "10.1016/j.cell.2016.05.072",
language = "English",
volume = "166",
pages = "343--357",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene Silencing in the C. elegans Germline

AU - Frøkjær-Jensen, Christian

AU - Jain, Nimit

AU - Hansen, Loren

AU - Davis, M Wayne

AU - Li, Yongbin

AU - Zhao, Di

AU - Rebora, Karine

AU - Millet, Jonathan R R M

AU - Liu, Xiao

AU - Kim, Stuart K

AU - Dupuy, Denis

AU - Jorgensen, Erik M

AU - Fire, Andrew Z.

PY - 2016/7/14

Y1 - 2016/7/14

N2 - Cells benefit from silencing foreign genetic elements but must simultaneously avoid inactivating endogenous genes. Although chromatin modifications and RNAs contribute to maintenance of silenced states, the establishment of silenced regions will inevitably reflect underlying DNA sequence and/or structure. Here, we demonstrate that a pervasive non-coding DNA feature in Caenorhabditis elegans, characterized by 10-base pair periodic An/Tn-clusters (PATCs), can license transgenes for germline expression within repressive chromatin domains. Transgenes containing natural or synthetic PATCs are resistant to position effect variegation and stochastic silencing in the germline. Among endogenous genes, intron length and PATC-character undergo dramatic changes as orthologs move from active to repressive chromatin over evolutionary time, indicating a dynamic character to the An/Tn periodicity. We propose that PATCs form the basis of a cellular immune system, identifying certain endogenous genes in heterochromatic contexts as privileged while foreign DNA can be suppressed with no requirement for a cellular memory of prior exposure.

AB - Cells benefit from silencing foreign genetic elements but must simultaneously avoid inactivating endogenous genes. Although chromatin modifications and RNAs contribute to maintenance of silenced states, the establishment of silenced regions will inevitably reflect underlying DNA sequence and/or structure. Here, we demonstrate that a pervasive non-coding DNA feature in Caenorhabditis elegans, characterized by 10-base pair periodic An/Tn-clusters (PATCs), can license transgenes for germline expression within repressive chromatin domains. Transgenes containing natural or synthetic PATCs are resistant to position effect variegation and stochastic silencing in the germline. Among endogenous genes, intron length and PATC-character undergo dramatic changes as orthologs move from active to repressive chromatin over evolutionary time, indicating a dynamic character to the An/Tn periodicity. We propose that PATCs form the basis of a cellular immune system, identifying certain endogenous genes in heterochromatic contexts as privileged while foreign DNA can be suppressed with no requirement for a cellular memory of prior exposure.

U2 - 10.1016/j.cell.2016.05.072

DO - 10.1016/j.cell.2016.05.072

M3 - Journal article

C2 - 27374334

AN - SCOPUS:84978413978

VL - 166

SP - 343

EP - 357

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -

ID: 178845139