A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis. / Engelholm, S A; Spang-Thomsen, M; Vindeløv, L L.

In: British Journal of Cancer, Vol. 47, No. 4, 1983, p. 497-502.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Engelholm, SA, Spang-Thomsen, M & Vindeløv, LL 1983, 'A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis', British Journal of Cancer, vol. 47, no. 4, pp. 497-502.

APA

Engelholm, S. A., Spang-Thomsen, M., & Vindeløv, L. L. (1983). A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis. British Journal of Cancer, 47(4), 497-502.

Vancouver

Engelholm SA, Spang-Thomsen M, Vindeløv LL. A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis. British Journal of Cancer. 1983;47(4):497-502.

Author

Engelholm, S A ; Spang-Thomsen, M ; Vindeløv, L L. / A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis. In: British Journal of Cancer. 1983 ; Vol. 47, No. 4. pp. 497-502.

Bibtex

@article{af7985a0656611de8bc9000ea68e967b,
title = "A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis",
abstract = "A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.",
author = "Engelholm, {S A} and M Spang-Thomsen and Vindel{\o}v, {L L}",
note = "Keywords: Animals; Carcinoma, Ehrlich Tumor; Cell Cycle; Cells, Cultured; DNA, Neoplasm; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance; Flow Cytometry; Mice",
year = "1983",
language = "English",
volume = "47",
pages = "497--502",
journal = "The British journal of cancer. Supplement",
issn = "0007-0920",
publisher = "nature publishing group",
number = "4",

}

RIS

TY - JOUR

T1 - A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis

AU - Engelholm, S A

AU - Spang-Thomsen, M

AU - Vindeløv, L L

N1 - Keywords: Animals; Carcinoma, Ehrlich Tumor; Cell Cycle; Cells, Cultured; DNA, Neoplasm; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance; Flow Cytometry; Mice

PY - 1983

Y1 - 1983

N2 - A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.

AB - A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.

M3 - Journal article

C2 - 6849794

VL - 47

SP - 497

EP - 502

JO - The British journal of cancer. Supplement

JF - The British journal of cancer. Supplement

SN - 0007-0920

IS - 4

ER -

ID: 12872558