Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity
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Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity. / Sogaard, Ditte; Ostergard, Torben; Blachnio-Zabielska, Agnieszka U.; Baranowski, Marcin; Vigelsø Hansen, Andreas; Andersen, Jesper Løvind; Dela, Flemming; Helge, Jorn Wulff.
In: Journal of Diabetes Research, Vol. 2016, 2372741, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity
AU - Sogaard, Ditte
AU - Ostergard, Torben
AU - Blachnio-Zabielska, Agnieszka U.
AU - Baranowski, Marcin
AU - Vigelsø Hansen, Andreas
AU - Andersen, Jesper Løvind
AU - Dela, Flemming
AU - Helge, Jorn Wulff
PY - 2016
Y1 - 2016
N2 - Ceramide and diacylglycerol (DAG) may be involved in the early phase of insulin resistance but data are inconsistent in man. We evaluated if an increase in insulin sensitivity after endurance training was accompanied by changes in these lipids in skeletal muscle. Nineteen first-degree type 2 diabetes Offsprings (Offsprings) (age: yrs; BMI: kg/m2) and sixteen matched Controls (age: yrs; BMI: kg/m2) performed 10 weeks of endurance training three times a week at 70% of VO2max on a bicycle ergometer. Before and after the intervention a hyperinsulinemic-euglycemic clamp and VO2max test were performed and muscle biopsies obtained. Insulin sensitivity was significantly lower in Offsprings compared to control subjects () but improved in both groups after 10 weeks of endurance training (Off: %; Con: %, ). The content of muscle ceramide, DAG, and their subspecies were similar between groups and did not change in response to the endurance training except for an overall reduction in C22:0-Cer (). Finally, the intervention induced an increase in AKT protein expression (Off: %; Con: %, ). This study showed no relation between insulin sensitivity and ceramide or DAG content suggesting that ceramide and DAG are not major players in the early phase of insulin resistance in human muscle.
AB - Ceramide and diacylglycerol (DAG) may be involved in the early phase of insulin resistance but data are inconsistent in man. We evaluated if an increase in insulin sensitivity after endurance training was accompanied by changes in these lipids in skeletal muscle. Nineteen first-degree type 2 diabetes Offsprings (Offsprings) (age: yrs; BMI: kg/m2) and sixteen matched Controls (age: yrs; BMI: kg/m2) performed 10 weeks of endurance training three times a week at 70% of VO2max on a bicycle ergometer. Before and after the intervention a hyperinsulinemic-euglycemic clamp and VO2max test were performed and muscle biopsies obtained. Insulin sensitivity was significantly lower in Offsprings compared to control subjects () but improved in both groups after 10 weeks of endurance training (Off: %; Con: %, ). The content of muscle ceramide, DAG, and their subspecies were similar between groups and did not change in response to the endurance training except for an overall reduction in C22:0-Cer (). Finally, the intervention induced an increase in AKT protein expression (Off: %; Con: %, ). This study showed no relation between insulin sensitivity and ceramide or DAG content suggesting that ceramide and DAG are not major players in the early phase of insulin resistance in human muscle.
U2 - 10.1155/2016/2372741
DO - 10.1155/2016/2372741
M3 - Journal article
C2 - 27777958
VL - 2016
JO - Journal of Diabetes Research
JF - Journal of Diabetes Research
SN - 2314-6745
M1 - 2372741
ER -
ID: 169416792