T cell subsets in human airways prior to and following endobronchial administration of endotoxin
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T cell subsets in human airways prior to and following endobronchial administration of endotoxin. / Ronit, Andreas; Plovsing, Ronni R; Gaardbo, Julie C; Berg, Ronan M G; Hartling, Hans J; Konge, Lars; Iversen, Martin; Ullum, Henrik; Møller, Kirsten; Nielsen, Susanne Dam.
In: Respirology, Vol. 20, No. 4, 2015, p. 579-86.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - T cell subsets in human airways prior to and following endobronchial administration of endotoxin
AU - Ronit, Andreas
AU - Plovsing, Ronni R
AU - Gaardbo, Julie C
AU - Berg, Ronan M G
AU - Hartling, Hans J
AU - Konge, Lars
AU - Iversen, Martin
AU - Ullum, Henrik
AU - Møller, Kirsten
AU - Nielsen, Susanne Dam
N1 - © 2015 Asian Pacific Society of Respirology.
PY - 2015
Y1 - 2015
N2 - BACKGROUND AND OBJECTIVES: Bronchial instillation of lipopolysaccharide (LPS) provides a reversible model of lung inflammation that may resemble early stages of acute respiratory distress syndrome (ARDS). We investigated the distributions of T-cell subsets in the human airways and sought to determine whether pro- and anti-inflammatory T cells are involved in the local immune response to lung inflammation.METHODS: Bronchoalveolar lavage (BAL) was performed in 15 healthy volunteers, after which Escherichia coli LPS (4 ng/kg) was administered. BAL was repeated at 2, 4, 6, 8 or 24 h after instillation of LPS.RESULTS: BALF CD4+ and CD8+ T cells were characterized by expression of activation markers (HLA-DR+CD38+), the proportion of cells expressing naïve markers (CD45RA+CD27+CCR7+) was lower, and that of cells expressing effector memory markers (CD45RA-CD27+CCR7-) was higher, compared with peripheral blood. Bronchial LPS induced a local inflammatory response with recruitment of CD4+ (P=0.014), CD8+ T cells (P=0.034), an increase in the proportion of CD4+CD25+CD127lowFoxp3+ regulatory T cells (Tregs) (P=0.045) and a tendency towards an increase in CD4+CD161+ cells (P=0.071) were observed.CONCLUSIONS: A unique distribution of T cells with little day-to-day variation was found in human airways. An increase in Tregs after endobronchial LPS suggests a role for Tregs during early stages of pulmonary inflammation.
AB - BACKGROUND AND OBJECTIVES: Bronchial instillation of lipopolysaccharide (LPS) provides a reversible model of lung inflammation that may resemble early stages of acute respiratory distress syndrome (ARDS). We investigated the distributions of T-cell subsets in the human airways and sought to determine whether pro- and anti-inflammatory T cells are involved in the local immune response to lung inflammation.METHODS: Bronchoalveolar lavage (BAL) was performed in 15 healthy volunteers, after which Escherichia coli LPS (4 ng/kg) was administered. BAL was repeated at 2, 4, 6, 8 or 24 h after instillation of LPS.RESULTS: BALF CD4+ and CD8+ T cells were characterized by expression of activation markers (HLA-DR+CD38+), the proportion of cells expressing naïve markers (CD45RA+CD27+CCR7+) was lower, and that of cells expressing effector memory markers (CD45RA-CD27+CCR7-) was higher, compared with peripheral blood. Bronchial LPS induced a local inflammatory response with recruitment of CD4+ (P=0.014), CD8+ T cells (P=0.034), an increase in the proportion of CD4+CD25+CD127lowFoxp3+ regulatory T cells (Tregs) (P=0.045) and a tendency towards an increase in CD4+CD161+ cells (P=0.071) were observed.CONCLUSIONS: A unique distribution of T cells with little day-to-day variation was found in human airways. An increase in Tregs after endobronchial LPS suggests a role for Tregs during early stages of pulmonary inflammation.
U2 - 10.1111/resp.12497
DO - 10.1111/resp.12497
M3 - Journal article
C2 - 25711164
VL - 20
SP - 579
EP - 586
JO - Respirology
JF - Respirology
SN - 1323-7799
IS - 4
ER -
ID: 143089376