Spontaneous and Evoked Contractility of Human Intestinal Lymphatic Vessels
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Spontaneous and Evoked Contractility of Human Intestinal Lymphatic Vessels. / Telinius, Niklas; Majgaard, Jens; Mohanakumar, Sheyanth; Pahle, Einar; Nielsen, Jørn; Hjortdal, Vibeke; Aalkjær, Christian; Boedtkjer, Donna Briggs.
In: Lymphatic Research and Biology, Vol. 15, No. 1, 03.2017, p. 17-22.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Spontaneous and Evoked Contractility of Human Intestinal Lymphatic Vessels
AU - Telinius, Niklas
AU - Majgaard, Jens
AU - Mohanakumar, Sheyanth
AU - Pahle, Einar
AU - Nielsen, Jørn
AU - Hjortdal, Vibeke
AU - Aalkjær, Christian
AU - Boedtkjer, Donna Briggs
PY - 2017/3
Y1 - 2017/3
N2 - BACKGROUND: Mesenteric lymphatic vessels (MLVs) from various animal species have been intensively studied. We aimed to establish the viability and basic contractile characteristics of human MLVs maintained in vitro and to determine the reactivity of MLVs with norepinephrine (NE) and substance P (SP) and to compare with the thoracic duct (TD).METHODS AND RESULTS: Isolated human lymphatic vessels were mounted on a wire myograph under isometric conditions and tension was recorded. The diameter-tension characteristics for MLVs were generated by stretching the vessels and stimulating with a 125 mM K+ solution containing 10 μM NE. The diameter-tension data generated for MLVs from two separate surgical patient groups were found to be similar: maximum active tension for MLVs occurred when the passive stretch corresponded to a transmural pressure of 22 mmHg. Subsequent experiments on human MLVs were performed by normalization with 22 mmHg as the equivalent target pressure. The majority of MLVs were responders (spontaneous activity and/or reactivity with 10 μM NE or 125 mM K+ solution). Nonresponders (16% of vessel segments) had significantly smaller inner diameters. MLVs responded consistently to NE (1 nM-10 μM) but the responsiveness of MLVs and TD to SP (0.1 nM-10 μM) was poor: TD reacted only with 10 μM SP, whereas MLVs were sensitive to nanomolar concentrations and the contractile response declined with higher concentrations.CONCLUSIONS: Under in vitro isometric conditions, human MLVs generate maximum tension when stretched to a passive level corresponding to 22 mmHg, and the majority of MLVs are responsive when normalized to this pressure. MLVs respond to NE and SP though NE produces a more consistent response in the concentration range tested.
AB - BACKGROUND: Mesenteric lymphatic vessels (MLVs) from various animal species have been intensively studied. We aimed to establish the viability and basic contractile characteristics of human MLVs maintained in vitro and to determine the reactivity of MLVs with norepinephrine (NE) and substance P (SP) and to compare with the thoracic duct (TD).METHODS AND RESULTS: Isolated human lymphatic vessels were mounted on a wire myograph under isometric conditions and tension was recorded. The diameter-tension characteristics for MLVs were generated by stretching the vessels and stimulating with a 125 mM K+ solution containing 10 μM NE. The diameter-tension data generated for MLVs from two separate surgical patient groups were found to be similar: maximum active tension for MLVs occurred when the passive stretch corresponded to a transmural pressure of 22 mmHg. Subsequent experiments on human MLVs were performed by normalization with 22 mmHg as the equivalent target pressure. The majority of MLVs were responders (spontaneous activity and/or reactivity with 10 μM NE or 125 mM K+ solution). Nonresponders (16% of vessel segments) had significantly smaller inner diameters. MLVs responded consistently to NE (1 nM-10 μM) but the responsiveness of MLVs and TD to SP (0.1 nM-10 μM) was poor: TD reacted only with 10 μM SP, whereas MLVs were sensitive to nanomolar concentrations and the contractile response declined with higher concentrations.CONCLUSIONS: Under in vitro isometric conditions, human MLVs generate maximum tension when stretched to a passive level corresponding to 22 mmHg, and the majority of MLVs are responsive when normalized to this pressure. MLVs respond to NE and SP though NE produces a more consistent response in the concentration range tested.
KW - Adult
KW - Dose-Response Relationship, Drug
KW - Humans
KW - Intestines/drug effects
KW - Isometric Contraction/drug effects
KW - Lymphatic Vessels/drug effects
KW - Mesentery/drug effects
KW - Middle Aged
KW - Muscle Contraction/drug effects
KW - Norepinephrine/pharmacology
KW - Substance P/pharmacology
KW - Vasoconstrictor Agents/pharmacology
KW - Young Adult
U2 - 10.1089/lrb.2016.0039
DO - 10.1089/lrb.2016.0039
M3 - Journal article
C2 - 28277905
VL - 15
SP - 17
EP - 22
JO - Lymphatic Research and Biology
JF - Lymphatic Research and Biology
SN - 1539-6851
IS - 1
ER -
ID: 246354704