Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo
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Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo. / Saxton, Sophie N.; Withers, Sarah B.; Nyvad, Jakob; Mazur, Aleksandra; Matchkov, Vladimir; Heagerty, Anthony M.; Aalkjaer, Christian.
In: Journal of Vascular Research, Vol. 56, No. 6, 2019, p. 320-332.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo
AU - Saxton, Sophie N.
AU - Withers, Sarah B.
AU - Nyvad, Jakob
AU - Mazur, Aleksandra
AU - Matchkov, Vladimir
AU - Heagerty, Anthony M.
AU - Aalkjaer, Christian
PY - 2019
Y1 - 2019
N2 - Background: Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte beta(3)-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin. Objective: We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of beta(3)-adrenoceptors and adiponectin. Method: In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach). Results: We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using beta(3)-adrenoceptor inhibition, and activation of beta(3)-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The beta(3)-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon beta(3)-adrenoceptor activation. Conclusions: These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte beta(3)-adrenoceptors, which may trigger release of the vasodilator adiponectin
AB - Background: Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte beta(3)-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin. Objective: We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of beta(3)-adrenoceptors and adiponectin. Method: In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach). Results: We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using beta(3)-adrenoceptor inhibition, and activation of beta(3)-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The beta(3)-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon beta(3)-adrenoceptor activation. Conclusions: These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte beta(3)-adrenoceptors, which may trigger release of the vasodilator adiponectin
KW - Adipocytes
KW - Adrenoceptors
KW - Vascular tone
U2 - 10.1159/000502689
DO - 10.1159/000502689
M3 - Journal article
C2 - 31550717
VL - 56
SP - 320
EP - 332
JO - Journal of Vascular Research
JF - Journal of Vascular Research
SN - 1018-1172
IS - 6
ER -
ID: 236717999