Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies

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Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies. / Larsen, R; Mertz-Nielsen, A; Hansen, M B; Poulsen, Steen Seier; Bindslev, N.

In: Acta physiologica Scandinavica, Vol. 173, No. 2, 10.2001, p. 213-222.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, R, Mertz-Nielsen, A, Hansen, MB, Poulsen, SS & Bindslev, N 2001, 'Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies', Acta physiologica Scandinavica, vol. 173, no. 2, pp. 213-222.

APA

Larsen, R., Mertz-Nielsen, A., Hansen, M. B., Poulsen, S. S., & Bindslev, N. (2001). Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies. Acta physiologica Scandinavica, 173(2), 213-222.

Vancouver

Larsen R, Mertz-Nielsen A, Hansen MB, Poulsen SS, Bindslev N. Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies. Acta physiologica Scandinavica. 2001 Oct;173(2):213-222.

Author

Larsen, R ; Mertz-Nielsen, A ; Hansen, M B ; Poulsen, Steen Seier ; Bindslev, N. / Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies. In: Acta physiologica Scandinavica. 2001 ; Vol. 173, No. 2. pp. 213-222.

Bibtex

@article{676619a074c711dbbee902004c4f4f50,
title = "Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies",
abstract = "The aim of this study was to design and evaluate a modified Ussing chamber, that makes use of constant air suction (modified Ussing air suction chamber, MUAS) for fixation of biopsy specimens. Standard size forceps biopsies were taken from the descending part of duodenum from patients undergoing endoscopy. Short circuit current (SCC) and conductance (G) were measured during basal conditions and after addition of different sugars and secretagogues. Histologic examination was performed to determine the degree of tissue damage after study in the chamber. Basal SCC was 54.7 +/- 4.3 microA x cm(-2) and G was 58.7 +/- 4.7 mS x cm(-2) (mean +/- SEM, n=48) and steady values of these parameters were observed for at least 2 h. Reproducible and steady responses in SCC were obtained with D-glucose (SCCmax=172 +/- 22.1 microA x cm(-2); EC50=6.9 +/- 0.7 mM, n=5) and D-galactose (SCCmax=233 +/- 55.7 microA x cm(-2); EC50=9.2 +/- 0.7 mM, n=3), and secretory responses with 5-hydroxytryptamine, 100 microM (DeltaSCC= 16.1 +/- 3.8 microA x cm(-2), n=10), histamine, 100 microM (DeltaSCC=24.0 +/- 4.1 microA x cm(-2), n=10) and prostaglandin E2, 1 microM (DeltaSCC=30.3 +/- 5.4 microA x cm(-2), n=6). Experimental biopsy specimens showed intact surface epithelium by histologic examination and did not differ from controls apart from minor indications of edge damage. No difference in basal electrical parameters and D-glucose fluxes were found between Helicobacter pylori positive and negative patients. Our data suggests that the MUAS chamber represents a promising alternative approach to measure transport processes in intestinal endoscopic biopsies.",
keywords = "Adult, Aged, Biopsy, Diffusion Chambers, Culture, Dinoprostone, Duodenum, Endoscopy, Digestive System, Fructose, Galactose, Glucose, Helicobacter Infections, Helicobacter pylori, Histamine, Humans, Intestinal Absorption, Intestinal Mucosa, Middle Aged, Serotonin, Water",
author = "R Larsen and A Mertz-Nielsen and Hansen, {M B} and Poulsen, {Steen Seier} and N Bindslev",
year = "2001",
month = oct,
language = "English",
volume = "173",
pages = "213--222",
journal = "Acta Physiologica Scandinavica",
issn = "0001-6772",
publisher = "Blackwell Science Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - Novel modified Ussing chamber for the study of absorption and secretion in human endoscopic biopsies

AU - Larsen, R

AU - Mertz-Nielsen, A

AU - Hansen, M B

AU - Poulsen, Steen Seier

AU - Bindslev, N

PY - 2001/10

Y1 - 2001/10

N2 - The aim of this study was to design and evaluate a modified Ussing chamber, that makes use of constant air suction (modified Ussing air suction chamber, MUAS) for fixation of biopsy specimens. Standard size forceps biopsies were taken from the descending part of duodenum from patients undergoing endoscopy. Short circuit current (SCC) and conductance (G) were measured during basal conditions and after addition of different sugars and secretagogues. Histologic examination was performed to determine the degree of tissue damage after study in the chamber. Basal SCC was 54.7 +/- 4.3 microA x cm(-2) and G was 58.7 +/- 4.7 mS x cm(-2) (mean +/- SEM, n=48) and steady values of these parameters were observed for at least 2 h. Reproducible and steady responses in SCC were obtained with D-glucose (SCCmax=172 +/- 22.1 microA x cm(-2); EC50=6.9 +/- 0.7 mM, n=5) and D-galactose (SCCmax=233 +/- 55.7 microA x cm(-2); EC50=9.2 +/- 0.7 mM, n=3), and secretory responses with 5-hydroxytryptamine, 100 microM (DeltaSCC= 16.1 +/- 3.8 microA x cm(-2), n=10), histamine, 100 microM (DeltaSCC=24.0 +/- 4.1 microA x cm(-2), n=10) and prostaglandin E2, 1 microM (DeltaSCC=30.3 +/- 5.4 microA x cm(-2), n=6). Experimental biopsy specimens showed intact surface epithelium by histologic examination and did not differ from controls apart from minor indications of edge damage. No difference in basal electrical parameters and D-glucose fluxes were found between Helicobacter pylori positive and negative patients. Our data suggests that the MUAS chamber represents a promising alternative approach to measure transport processes in intestinal endoscopic biopsies.

AB - The aim of this study was to design and evaluate a modified Ussing chamber, that makes use of constant air suction (modified Ussing air suction chamber, MUAS) for fixation of biopsy specimens. Standard size forceps biopsies were taken from the descending part of duodenum from patients undergoing endoscopy. Short circuit current (SCC) and conductance (G) were measured during basal conditions and after addition of different sugars and secretagogues. Histologic examination was performed to determine the degree of tissue damage after study in the chamber. Basal SCC was 54.7 +/- 4.3 microA x cm(-2) and G was 58.7 +/- 4.7 mS x cm(-2) (mean +/- SEM, n=48) and steady values of these parameters were observed for at least 2 h. Reproducible and steady responses in SCC were obtained with D-glucose (SCCmax=172 +/- 22.1 microA x cm(-2); EC50=6.9 +/- 0.7 mM, n=5) and D-galactose (SCCmax=233 +/- 55.7 microA x cm(-2); EC50=9.2 +/- 0.7 mM, n=3), and secretory responses with 5-hydroxytryptamine, 100 microM (DeltaSCC= 16.1 +/- 3.8 microA x cm(-2), n=10), histamine, 100 microM (DeltaSCC=24.0 +/- 4.1 microA x cm(-2), n=10) and prostaglandin E2, 1 microM (DeltaSCC=30.3 +/- 5.4 microA x cm(-2), n=6). Experimental biopsy specimens showed intact surface epithelium by histologic examination and did not differ from controls apart from minor indications of edge damage. No difference in basal electrical parameters and D-glucose fluxes were found between Helicobacter pylori positive and negative patients. Our data suggests that the MUAS chamber represents a promising alternative approach to measure transport processes in intestinal endoscopic biopsies.

KW - Adult

KW - Aged

KW - Biopsy

KW - Diffusion Chambers, Culture

KW - Dinoprostone

KW - Duodenum

KW - Endoscopy, Digestive System

KW - Fructose

KW - Galactose

KW - Glucose

KW - Helicobacter Infections

KW - Helicobacter pylori

KW - Histamine

KW - Humans

KW - Intestinal Absorption

KW - Intestinal Mucosa

KW - Middle Aged

KW - Serotonin

KW - Water

M3 - Journal article

C2 - 11683679

VL - 173

SP - 213

EP - 222

JO - Acta Physiologica Scandinavica

JF - Acta Physiologica Scandinavica

SN - 0001-6772

IS - 2

ER -

ID: 168074