Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy
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Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy. / Brazhe, Nadezda A; Treiman, Marek; Brazhe, Alexey R; Find, Ninett L; Maksimov, Georgy V; Sosnovtseva, Olga.
In: P L o S One, Vol. 7, No. 9, 2012, p. e41990.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy
AU - Brazhe, Nadezda A
AU - Treiman, Marek
AU - Brazhe, Alexey R
AU - Find, Ninett L
AU - Maksimov, Georgy V
AU - Sosnovtseva, Olga
PY - 2012
Y1 - 2012
N2 - This paper presents a nonivasive approach to study redox state of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Formula: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.
AB - This paper presents a nonivasive approach to study redox state of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Formula: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.
U2 - 10.1371/journal.pone.0041990
DO - 10.1371/journal.pone.0041990
M3 - Journal article
C2 - 22957018
VL - 7
SP - e41990
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 9
ER -
ID: 40869572