Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy

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Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy. / Brazhe, Nadezda A; Treiman, Marek; Brazhe, Alexey R; Find, Ninett L; Maksimov, Georgy V; Sosnovtseva, Olga.

In: P L o S One, Vol. 7, No. 9, 2012, p. e41990.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Brazhe, NA, Treiman, M, Brazhe, AR, Find, NL, Maksimov, GV & Sosnovtseva, O 2012, 'Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy', P L o S One, vol. 7, no. 9, pp. e41990. https://doi.org/10.1371/journal.pone.0041990

APA

Brazhe, N. A., Treiman, M., Brazhe, A. R., Find, N. L., Maksimov, G. V., & Sosnovtseva, O. (2012). Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy. P L o S One, 7(9), e41990. https://doi.org/10.1371/journal.pone.0041990

Vancouver

Brazhe NA, Treiman M, Brazhe AR, Find NL, Maksimov GV, Sosnovtseva O. Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy. P L o S One. 2012;7(9):e41990. https://doi.org/10.1371/journal.pone.0041990

Author

Brazhe, Nadezda A ; Treiman, Marek ; Brazhe, Alexey R ; Find, Ninett L ; Maksimov, Georgy V ; Sosnovtseva, Olga. / Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy. In: P L o S One. 2012 ; Vol. 7, No. 9. pp. e41990.

Bibtex

@article{b3b350b0b97246b58e689360d34c13d0,
title = "Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy",
abstract = "This paper presents a nonivasive approach to study redox state of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Formula: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.",
author = "Brazhe, {Nadezda A} and Marek Treiman and Brazhe, {Alexey R} and Find, {Ninett L} and Maksimov, {Georgy V} and Olga Sosnovtseva",
year = "2012",
doi = "10.1371/journal.pone.0041990",
language = "English",
volume = "7",
pages = "e41990",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy

AU - Brazhe, Nadezda A

AU - Treiman, Marek

AU - Brazhe, Alexey R

AU - Find, Ninett L

AU - Maksimov, Georgy V

AU - Sosnovtseva, Olga

PY - 2012

Y1 - 2012

N2 - This paper presents a nonivasive approach to study redox state of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Formula: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.

AB - This paper presents a nonivasive approach to study redox state of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Formula: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.

U2 - 10.1371/journal.pone.0041990

DO - 10.1371/journal.pone.0041990

M3 - Journal article

C2 - 22957018

VL - 7

SP - e41990

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

ER -

ID: 40869572