Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum. / Rasmussen, T N; Schmidt, P; Poulsen, S S; Holst, J J; Poulsen, Steen Seier.

In: Regulatory Peptides, Vol. 98, No. 3, 20.04.2001, p. 137-143.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rasmussen, TN, Schmidt, P, Poulsen, SS, Holst, JJ & Poulsen, SS 2001, 'Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum', Regulatory Peptides, vol. 98, no. 3, pp. 137-143.

APA

Rasmussen, T. N., Schmidt, P., Poulsen, S. S., Holst, J. J., & Poulsen, S. S. (2001). Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum. Regulatory Peptides, 98(3), 137-143.

Vancouver

Rasmussen TN, Schmidt P, Poulsen SS, Holst JJ, Poulsen SS. Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum. Regulatory Peptides. 2001 Apr 20;98(3):137-143.

Author

Rasmussen, T N ; Schmidt, P ; Poulsen, S S ; Holst, J J ; Poulsen, Steen Seier. / Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum. In: Regulatory Peptides. 2001 ; Vol. 98, No. 3. pp. 137-143.

Bibtex

@article{775a448074c711dbbee902004c4f4f50,
title = "Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum",
abstract = "By immunohistochemistry, CGRP-like immunoreactive (CGRP-LI) nerve fibres were found in the lamina propria along small vessels and in the lamina muscularis mucosae in the porcine ileum. Immunoreactive nerve cell bodies were found in the submucous and myenteric plexus. Upon HPLC-analysis of ileal extracts, CGRP-LI corresponded entirely to porcine CGRP plus smaller amounts of oxidised CGRP. Using isolated vascularly perfused segments of the ileum, we studied the release of CGRP-LI in response to electrical stimulation of the mixed extrinsic periarterial nerves and to infusion of different neuroblockers. In addition, the effect of infusion of capsaicin was studied. The basal output of CGRP-LI was 2.9+/-0.7 pmol/5 min (mean+/-S.D.). Electrical nerve stimulation (8 Hz) significantly increased the release of CGRP-LI to 167+/-16% (mean+/-S.E.M.) of the basal output (n=13). This response was unaffected by the addition of atropine (10(-6) M). Nerve stimulation during infusion of phentolamine (10(-5) M) with and without additional infusion of atropine resulted in a significant further increase in the release of CGRP-LI to 261+/-134% (n=5) and 240+/-80% (n=9), respectively. This response was abolished by infusion of hexamethonium (3x10(-5) M). Infusion of capsaicin (10(-5) M) caused a significant increase in the release of CGRP-LI to 485+/-82% of basal output (n=5). Our results suggest a dual origin of CGRP innervation of the porcine ileum (intrinsic and extrinsic). The intrinsic CGRP neurons receive excitatory input by parasympathetic, possibly vagal, preganglionic fibres, via release of acetylcholine acting on nicotinic receptors. The stimulatory effect of capsaicin suggests that CGRP is also released from extrinsic sensory neurons.",
keywords = "Animals, Atropine, Calcitonin Gene-Related Peptide, Capsaicin, Chromatography, High Pressure Liquid, Electric Stimulation, Ganglionic Blockers, Hexamethonium, Ileum, Immunohistochemistry, Microscopy, Fluorescence, Myenteric Plexus, Nerve Fibers, Perfusion, Phentolamine, Radioimmunoassay, Spinal Cord, Submucous Plexus, Swine",
author = "Rasmussen, {T N} and P Schmidt and Poulsen, {S S} and Holst, {J J} and Poulsen, {Steen Seier}",
year = "2001",
month = apr,
day = "20",
language = "English",
volume = "98",
pages = "137--143",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Localisation and neural control of the release of calcitonin gene-related peptide (CGRP) from the isolated perfused porcine ileum

AU - Rasmussen, T N

AU - Schmidt, P

AU - Poulsen, S S

AU - Holst, J J

AU - Poulsen, Steen Seier

PY - 2001/4/20

Y1 - 2001/4/20

N2 - By immunohistochemistry, CGRP-like immunoreactive (CGRP-LI) nerve fibres were found in the lamina propria along small vessels and in the lamina muscularis mucosae in the porcine ileum. Immunoreactive nerve cell bodies were found in the submucous and myenteric plexus. Upon HPLC-analysis of ileal extracts, CGRP-LI corresponded entirely to porcine CGRP plus smaller amounts of oxidised CGRP. Using isolated vascularly perfused segments of the ileum, we studied the release of CGRP-LI in response to electrical stimulation of the mixed extrinsic periarterial nerves and to infusion of different neuroblockers. In addition, the effect of infusion of capsaicin was studied. The basal output of CGRP-LI was 2.9+/-0.7 pmol/5 min (mean+/-S.D.). Electrical nerve stimulation (8 Hz) significantly increased the release of CGRP-LI to 167+/-16% (mean+/-S.E.M.) of the basal output (n=13). This response was unaffected by the addition of atropine (10(-6) M). Nerve stimulation during infusion of phentolamine (10(-5) M) with and without additional infusion of atropine resulted in a significant further increase in the release of CGRP-LI to 261+/-134% (n=5) and 240+/-80% (n=9), respectively. This response was abolished by infusion of hexamethonium (3x10(-5) M). Infusion of capsaicin (10(-5) M) caused a significant increase in the release of CGRP-LI to 485+/-82% of basal output (n=5). Our results suggest a dual origin of CGRP innervation of the porcine ileum (intrinsic and extrinsic). The intrinsic CGRP neurons receive excitatory input by parasympathetic, possibly vagal, preganglionic fibres, via release of acetylcholine acting on nicotinic receptors. The stimulatory effect of capsaicin suggests that CGRP is also released from extrinsic sensory neurons.

AB - By immunohistochemistry, CGRP-like immunoreactive (CGRP-LI) nerve fibres were found in the lamina propria along small vessels and in the lamina muscularis mucosae in the porcine ileum. Immunoreactive nerve cell bodies were found in the submucous and myenteric plexus. Upon HPLC-analysis of ileal extracts, CGRP-LI corresponded entirely to porcine CGRP plus smaller amounts of oxidised CGRP. Using isolated vascularly perfused segments of the ileum, we studied the release of CGRP-LI in response to electrical stimulation of the mixed extrinsic periarterial nerves and to infusion of different neuroblockers. In addition, the effect of infusion of capsaicin was studied. The basal output of CGRP-LI was 2.9+/-0.7 pmol/5 min (mean+/-S.D.). Electrical nerve stimulation (8 Hz) significantly increased the release of CGRP-LI to 167+/-16% (mean+/-S.E.M.) of the basal output (n=13). This response was unaffected by the addition of atropine (10(-6) M). Nerve stimulation during infusion of phentolamine (10(-5) M) with and without additional infusion of atropine resulted in a significant further increase in the release of CGRP-LI to 261+/-134% (n=5) and 240+/-80% (n=9), respectively. This response was abolished by infusion of hexamethonium (3x10(-5) M). Infusion of capsaicin (10(-5) M) caused a significant increase in the release of CGRP-LI to 485+/-82% of basal output (n=5). Our results suggest a dual origin of CGRP innervation of the porcine ileum (intrinsic and extrinsic). The intrinsic CGRP neurons receive excitatory input by parasympathetic, possibly vagal, preganglionic fibres, via release of acetylcholine acting on nicotinic receptors. The stimulatory effect of capsaicin suggests that CGRP is also released from extrinsic sensory neurons.

KW - Animals

KW - Atropine

KW - Calcitonin Gene-Related Peptide

KW - Capsaicin

KW - Chromatography, High Pressure Liquid

KW - Electric Stimulation

KW - Ganglionic Blockers

KW - Hexamethonium

KW - Ileum

KW - Immunohistochemistry

KW - Microscopy, Fluorescence

KW - Myenteric Plexus

KW - Nerve Fibers

KW - Perfusion

KW - Phentolamine

KW - Radioimmunoassay

KW - Spinal Cord

KW - Submucous Plexus

KW - Swine

M3 - Journal article

C2 - 11231043

VL - 98

SP - 137

EP - 143

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 3

ER -

ID: 168976