Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques
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Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques. / Faurschou, Annesofie; Pedersen, Jens; Gyldenløve, Mette; Poulsen, Steen Seier; Holst, Jens J; Thyssen, Jacob P; Zachariae, Claus; Vilsbøll, Tina; Skov, Lone; Knop, Filip K.
In: Experimental Dermatology, Vol. 22, No. 2, 02.2013, p. 150-2.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Increased expression of glucagon-like peptide-1 receptors in psoriasis plaques
AU - Faurschou, Annesofie
AU - Pedersen, Jens
AU - Gyldenløve, Mette
AU - Poulsen, Steen Seier
AU - Holst, Jens J
AU - Thyssen, Jacob P
AU - Zachariae, Claus
AU - Vilsbøll, Tina
AU - Skov, Lone
AU - Knop, Filip K
N1 - © 2013 John Wiley & Sons A/S.
PY - 2013/2
Y1 - 2013/2
N2 - Recent case reports suggest that treatment with glucagon-like peptide-1 (GLP-1) agonists results in clinical improvement of psoriasis. The purpose of this study was to determine whether GLP-1 receptors (GLP-1Rs) are found in the skin of healthy volunteers and psoriasis patients and if so, whether GLP-1Rs are located on keratinocytes or immune cells. Three mm-punch skin biopsies were taken for gene expression analysis from six healthy volunteers and from affected and unaffected skin of six psoriasis patients. In addition, a blood sample was obtained from all participants. Cultured human keratinocytes were either untreated or incubated with tumor necrosis factor- a (TNF-a), interferon-¿ (IFN-¿) or a combination of TNF-a and IFN-¿ for 48 h. Total RNA was extracted from all the samples, reversely transcribed and analysed for the expression of GLP-1R using real-time PCR. Gene expression analysis showed expression of GLP-1Rs in five of six skin biopsies from psoriasis plaques, in one of six biopsies from unaffected psoriatic skin and in one of six biopsies from healthy skin. GLP-1R expression was found in the blood of both healthy volunteers and psoriasis patients. No GLP-1R expression was found in either stimulated or unstimulated cultured human keratinocytes. Our results show increased presence of GLP-1Rs in psoriasis plaques and that this most likely is due to infiltration with immune cells. This offers a possible explanation for the positive effect of treatment with GLP-1R agonists in patients with psoriasis.
AB - Recent case reports suggest that treatment with glucagon-like peptide-1 (GLP-1) agonists results in clinical improvement of psoriasis. The purpose of this study was to determine whether GLP-1 receptors (GLP-1Rs) are found in the skin of healthy volunteers and psoriasis patients and if so, whether GLP-1Rs are located on keratinocytes or immune cells. Three mm-punch skin biopsies were taken for gene expression analysis from six healthy volunteers and from affected and unaffected skin of six psoriasis patients. In addition, a blood sample was obtained from all participants. Cultured human keratinocytes were either untreated or incubated with tumor necrosis factor- a (TNF-a), interferon-¿ (IFN-¿) or a combination of TNF-a and IFN-¿ for 48 h. Total RNA was extracted from all the samples, reversely transcribed and analysed for the expression of GLP-1R using real-time PCR. Gene expression analysis showed expression of GLP-1Rs in five of six skin biopsies from psoriasis plaques, in one of six biopsies from unaffected psoriatic skin and in one of six biopsies from healthy skin. GLP-1R expression was found in the blood of both healthy volunteers and psoriasis patients. No GLP-1R expression was found in either stimulated or unstimulated cultured human keratinocytes. Our results show increased presence of GLP-1Rs in psoriasis plaques and that this most likely is due to infiltration with immune cells. This offers a possible explanation for the positive effect of treatment with GLP-1R agonists in patients with psoriasis.
U2 - 10.1111/exd.12081
DO - 10.1111/exd.12081
M3 - Journal article
C2 - 23362875
VL - 22
SP - 150
EP - 152
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
IS - 2
ER -
ID: 45047726