Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats
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Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats. / Orskov, C; Poulsen, Steen Seier.
In: Diabetes, Vol. 40, No. 10, 10.1991, p. 1292-6.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats
AU - Orskov, C
AU - Poulsen, Steen Seier
PY - 1991/10
Y1 - 1991/10
N2 - We demonstrated specific binding of the insulin-releasing hormone glucagonlike peptide (GLP)-I-(7-36)-amide, an intestinal product of proglucagon, to pancreatic islet cells by autoradiography using 125I-labeled GLP-I-(7-36)-amide incubated with tissue specimens of extracerebral rat organs. We also found binding of 125I-GLP-I to insulin-, glucagon-, and somatostatin-immunoreactive cells by combined autoradiographic and immunohistochemical analysis of pancreatic specimens using antisera against insulin, glucagon, and somatostatin. An accumulation of radioactivity was also observed in the stomach surface epithelium but could not be prevented by excess unlabeled peptide. No binding was found in other tissues investigated, including the lungs and the small intestinal mucosa. Localization of the binding sites identifies the pancreatic islets as the prime target for GLP-I-(7-36)-amide and suggests that it exerts direct effects on islet cells.
AB - We demonstrated specific binding of the insulin-releasing hormone glucagonlike peptide (GLP)-I-(7-36)-amide, an intestinal product of proglucagon, to pancreatic islet cells by autoradiography using 125I-labeled GLP-I-(7-36)-amide incubated with tissue specimens of extracerebral rat organs. We also found binding of 125I-GLP-I to insulin-, glucagon-, and somatostatin-immunoreactive cells by combined autoradiographic and immunohistochemical analysis of pancreatic specimens using antisera against insulin, glucagon, and somatostatin. An accumulation of radioactivity was also observed in the stomach surface epithelium but could not be prevented by excess unlabeled peptide. No binding was found in other tissues investigated, including the lungs and the small intestinal mucosa. Localization of the binding sites identifies the pancreatic islets as the prime target for GLP-I-(7-36)-amide and suggests that it exerts direct effects on islet cells.
KW - Animals
KW - Autoradiography
KW - Female
KW - Glucagon
KW - Glucagon-Like Peptide 1
KW - Glucagon-Like Peptides
KW - Iodine Radioisotopes
KW - Islets of Langerhans
KW - Male
KW - Peptide Fragments
KW - Peptides
KW - Radioligand Assay
KW - Rats
KW - Rats, Inbred Strains
KW - Receptors, Cell Surface
KW - Receptors, Glucagon
KW - Stomach
M3 - Journal article
C2 - 1657666
VL - 40
SP - 1292
EP - 1296
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 10
ER -
ID: 47487806