Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves. / Taylor-Edwards, C C; Burrin, D G; Holst, Jens Juul; McLeod, K R; Harmon, D L.

In: Journal of Dairy Science, Vol. 94, No. 2, 2011, p. 888-898.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Taylor-Edwards, CC, Burrin, DG, Holst, JJ, McLeod, KR & Harmon, DL 2011, 'Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves', Journal of Dairy Science, vol. 94, no. 2, pp. 888-898. https://doi.org/10.3168/jds.2010-3540

APA

Taylor-Edwards, C. C., Burrin, D. G., Holst, J. J., McLeod, K. R., & Harmon, D. L. (2011). Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves. Journal of Dairy Science, 94(2), 888-898. https://doi.org/10.3168/jds.2010-3540

Vancouver

Taylor-Edwards CC, Burrin DG, Holst JJ, McLeod KR, Harmon DL. Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves. Journal of Dairy Science. 2011;94(2):888-898. https://doi.org/10.3168/jds.2010-3540

Author

Taylor-Edwards, C C ; Burrin, D G ; Holst, Jens Juul ; McLeod, K R ; Harmon, D L. / Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves. In: Journal of Dairy Science. 2011 ; Vol. 94, No. 2. pp. 888-898.

Bibtex

@article{9524d4901fca4cab9cf67ef5d9948c29,

title = "Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves",

abstract = "Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in nonruminants but its effect in ruminants is unknown. Eight Holstein calves with an ultrasonic flow probe around the superior mesenteric artery and catheters in the carotid artery and mesenteric vein were paired by age and randomly assigned to treatment of a control (0.5% of BSA in saline; n=4) or GLP-2 (50 µg/kg of body weight of bovine GLP-2 in BSA; n=4) given subcutaneously every 12h for 10 d. Blood flow was measured on d 0 (acute) and d 10 (chronic) and included 3 periods: baseline (saline infusion), treatment (infusion of BSA or 1,000 pmol of GLP-2/kg of body weight per h), and recovery (saline infusion). On d 11, calves were killed 2h after injection of 5-bromo-2'-deoxyuridine (BrdU). Gastrointestinal tissues were weighed and epithelial samples were obtained to determine villus height, crypt depth, and BrdU staining. Infusion of GLP-2 increased superior mesenteric artery blood flow to 175% of baseline on d 0 but to only 137% of baseline after chronic treatment. Compared with that of the control, GLP-2 increased small intestinal mass by 24% by increasing epithelial mass in the jejunum and ileum. Additionally, GLP-2 increased villus height, crypt depth, and BrdU-labeling in small intestinal segments. These results demonstrate that GLP-2 induces similar increases in small intestinal blood flow and growth in ruminants to those observed in nonruminants. Furthermore, GLP-2 increases small intestinal blood flow in ruminants but this response is attenuated after 10 d of GLP-2 administration. In cattle, GLP-2 may be an important hormone in the regulation of intestinal blood flow and epithelial growth.",

keywords = "Animals, Cattle, Glucagon-Like Peptide 2, Intestinal Mucosa, Intestine, Small, Mesenteric Artery, Superior, Regional Blood Flow, Time Factors",

author = "Taylor-Edwards, {C C} and Burrin, {D G} and Holst, {Jens Juul} and McLeod, {K R} and Harmon, {D L}",

year = "2011",

doi = "10.3168/jds.2010-3540",

language = "English",

volume = "94",

pages = "888--898",

journal = "Journal of Dairy Science",

issn = "0022-0302",

publisher = "Elsevier",

number = "2",

}

RIS

TY - JOUR

T1 - Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves

AU - Taylor-Edwards, C C

AU - Burrin, D G

AU - Holst, Jens Juul

AU - McLeod, K R

AU - Harmon, D L

PY - 2011

Y1 - 2011

N2 - Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in nonruminants but its effect in ruminants is unknown. Eight Holstein calves with an ultrasonic flow probe around the superior mesenteric artery and catheters in the carotid artery and mesenteric vein were paired by age and randomly assigned to treatment of a control (0.5% of BSA in saline; n=4) or GLP-2 (50 µg/kg of body weight of bovine GLP-2 in BSA; n=4) given subcutaneously every 12h for 10 d. Blood flow was measured on d 0 (acute) and d 10 (chronic) and included 3 periods: baseline (saline infusion), treatment (infusion of BSA or 1,000 pmol of GLP-2/kg of body weight per h), and recovery (saline infusion). On d 11, calves were killed 2h after injection of 5-bromo-2'-deoxyuridine (BrdU). Gastrointestinal tissues were weighed and epithelial samples were obtained to determine villus height, crypt depth, and BrdU staining. Infusion of GLP-2 increased superior mesenteric artery blood flow to 175% of baseline on d 0 but to only 137% of baseline after chronic treatment. Compared with that of the control, GLP-2 increased small intestinal mass by 24% by increasing epithelial mass in the jejunum and ileum. Additionally, GLP-2 increased villus height, crypt depth, and BrdU-labeling in small intestinal segments. These results demonstrate that GLP-2 induces similar increases in small intestinal blood flow and growth in ruminants to those observed in nonruminants. Furthermore, GLP-2 increases small intestinal blood flow in ruminants but this response is attenuated after 10 d of GLP-2 administration. In cattle, GLP-2 may be an important hormone in the regulation of intestinal blood flow and epithelial growth.

AB - Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in nonruminants but its effect in ruminants is unknown. Eight Holstein calves with an ultrasonic flow probe around the superior mesenteric artery and catheters in the carotid artery and mesenteric vein were paired by age and randomly assigned to treatment of a control (0.5% of BSA in saline; n=4) or GLP-2 (50 µg/kg of body weight of bovine GLP-2 in BSA; n=4) given subcutaneously every 12h for 10 d. Blood flow was measured on d 0 (acute) and d 10 (chronic) and included 3 periods: baseline (saline infusion), treatment (infusion of BSA or 1,000 pmol of GLP-2/kg of body weight per h), and recovery (saline infusion). On d 11, calves were killed 2h after injection of 5-bromo-2'-deoxyuridine (BrdU). Gastrointestinal tissues were weighed and epithelial samples were obtained to determine villus height, crypt depth, and BrdU staining. Infusion of GLP-2 increased superior mesenteric artery blood flow to 175% of baseline on d 0 but to only 137% of baseline after chronic treatment. Compared with that of the control, GLP-2 increased small intestinal mass by 24% by increasing epithelial mass in the jejunum and ileum. Additionally, GLP-2 increased villus height, crypt depth, and BrdU-labeling in small intestinal segments. These results demonstrate that GLP-2 induces similar increases in small intestinal blood flow and growth in ruminants to those observed in nonruminants. Furthermore, GLP-2 increases small intestinal blood flow in ruminants but this response is attenuated after 10 d of GLP-2 administration. In cattle, GLP-2 may be an important hormone in the regulation of intestinal blood flow and epithelial growth.

KW - Animals

KW - Cattle

KW - Glucagon-Like Peptide 2

KW - Intestinal Mucosa

KW - Intestine, Small

KW - Mesenteric Artery, Superior

KW - Regional Blood Flow

KW - Time Factors

U2 - 10.3168/jds.2010-3540

DO - 10.3168/jds.2010-3540

M3 - Journal article

C2 - 21257057

VL - 94

SP - 888

EP - 898

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 2

ER -

ID: 38531903

Department of Biomedical Sciences