Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions
Research output: Contribution to journal › Journal article › Research › peer-review
A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.
|Journal||European Journal of Pharmaceutical Sciences|
|Number of pages||11|
|Publication status||Published - 2012|