Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions

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A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.
Original languageEnglish
JournalEuropean Journal of Pharmaceutical Sciences
Issue number4
Pages (from-to)222-32
Number of pages11
Publication statusPublished - 2012

ID: 38229193