Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells
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Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells. / Petersen, Elisabeth D.; Billestrup, N; Nielsen, Jens Høiriis.
In: Biomedica Biochimica Acta, Vol. 49, No. 12, 1990, p. 1171-5.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells
AU - Petersen, Elisabeth D.
AU - Billestrup, N
AU - Nielsen, Jens Høiriis
PY - 1990
Y1 - 1990
N2 - Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.
AB - Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.
KW - Adenylate Cyclase
KW - Animals
KW - Blood
KW - Cycloheximide
KW - DNA Probes
KW - DNA-Binding Proteins
KW - Enzyme Activation
KW - Forskolin
KW - Gene Expression Regulation
KW - Insulin
KW - Insulinoma
KW - Phorbol 12,13-Dibutyrate
KW - Protein Kinase C
KW - Protein-Tyrosine Kinases
KW - Proto-Oncogene Proteins
KW - Proto-Oncogene Proteins c-fos
KW - Proto-Oncogene Proteins c-jun
KW - RNA, Messenger
KW - Rats
KW - Somatomedins
KW - Transcription Factors
KW - Tumor Cells, Cultured
M3 - Journal article
C2 - 2129102
VL - 49
SP - 1171
EP - 1175
JO - Biomedica Biochimica Acta
JF - Biomedica Biochimica Acta
SN - 0232-766X
IS - 12
ER -
ID: 47974133