Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses. / Rohde, Ulrich; Sonne, David Peick; Christensen, Mikkel; Hansen, Morten; Brønden, Andreas; Toräng, Signe; Rehfeld, Jens Frederik; Holst, Jens Juul; Lauritsen, Tina Vilsbøll; Knop, Filip Krag.

In: The Journal of clinical endocrinology and metabolism, Vol. 101, No. 5, 01.05.2016, p. 2076-2083.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rohde, U, Sonne, DP, Christensen, M, Hansen, M, Brønden, A, Toräng, S, Rehfeld, JF, Holst, JJ, Lauritsen, TV & Knop, FK 2016, 'Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses', The Journal of clinical endocrinology and metabolism, vol. 101, no. 5, pp. 2076-2083. https://doi.org/10.1210/jc.2016-1133

APA

Rohde, U., Sonne, D. P., Christensen, M., Hansen, M., Brønden, A., Toräng, S., Rehfeld, J. F., Holst, J. J., Lauritsen, T. V., & Knop, F. K. (2016). Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses. The Journal of clinical endocrinology and metabolism, 101(5), 2076-2083. https://doi.org/10.1210/jc.2016-1133

Vancouver

Rohde U, Sonne DP, Christensen M, Hansen M, Brønden A, Toräng S et al. Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses. The Journal of clinical endocrinology and metabolism. 2016 May 1;101(5):2076-2083. https://doi.org/10.1210/jc.2016-1133

Author

Rohde, Ulrich ; Sonne, David Peick ; Christensen, Mikkel ; Hansen, Morten ; Brønden, Andreas ; Toräng, Signe ; Rehfeld, Jens Frederik ; Holst, Jens Juul ; Lauritsen, Tina Vilsbøll ; Knop, Filip Krag. / Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses. In: The Journal of clinical endocrinology and metabolism. 2016 ; Vol. 101, No. 5. pp. 2076-2083.

Bibtex

@article{ffef3deff2de40b18b94a4e725aa377b,
title = "Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses",
abstract = "CONTEXT: Bile acids have been suggested to mediate glucagon-like peptide-1 (GLP-1) secretion via activation of the bile acid receptor TGR5 on enteroendocrine L cells. Metformin too has been shown to increase GLP-1 levels. The effect of gallbladder emptying, metformin or a combination has however never been studied.OBJECTIVE: We hypothesized that cholecystokinin-8 (CCK)-induced gallbladder emptying stimulates human GLP-1 secretion and that metformin would potentiate this effect.DESIGN: A double-blinded, randomized study.SETTING: The study was conducted at a specialized research unit.PARTICIPANTS: Ten healthy male subjects with no family history of diabetes (aged 22 (range 20-32) years; body mass index 21.7 (19.3-24.2) kg/m(2); fasting plasma glucose 4.9 (4.7-5.3) mM; and HbA1c 5.1 (4.4-5.8) %).INTERVENTION: On 4 separate days, the subjects received metformin or placebo and a concomitant 60-minute intravenous infusion of saline or CCK. Blood was sampled for 4 hours and gallbladder volume measured by ultrasound.MAIN OUTCOME MEASURES: Plasma levels of GLP-1.RESULTS: CCK-induced gallbladder emptying and metformin alone (no observed effect on gallbladder emptying), both elicited significant and additive GLP-1 responses. Metformin alone or combined with gallbladder emptying elicited a significant peptide YY response. CCK-induced gallbladder emptying resulted in a short-lasting glucose-dependent insulinotropic polypeptide (GIP) response independent of metformin. No effects were seen on plasma glucose, insulin, C-peptide or gastrin.CONCLUSIONS: CCK-induced gallbladder emptying in healthy subjects elicits significant GLP-1 secretion, which can be potentiated by metformin.",
author = "Ulrich Rohde and Sonne, {David Peick} and Mikkel Christensen and Morten Hansen and Andreas Br{\o}nden and Signe Tor{\"a}ng and Rehfeld, {Jens Frederik} and Holst, {Jens Juul} and Lauritsen, {Tina Vilsb{\o}ll} and Knop, {Filip Krag}",
year = "2016",
month = may,
day = "1",
doi = "10.1210/jc.2016-1133",
language = "English",
volume = "101",
pages = "2076--2083",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Cholecystokinin-Induced Gallbladder Emptying and Single-Dose Metformin Elicit Additive Glucagon-Like Peptide-1 Responses

AU - Rohde, Ulrich

AU - Sonne, David Peick

AU - Christensen, Mikkel

AU - Hansen, Morten

AU - Brønden, Andreas

AU - Toräng, Signe

AU - Rehfeld, Jens Frederik

AU - Holst, Jens Juul

AU - Lauritsen, Tina Vilsbøll

AU - Knop, Filip Krag

PY - 2016/5/1

Y1 - 2016/5/1

N2 - CONTEXT: Bile acids have been suggested to mediate glucagon-like peptide-1 (GLP-1) secretion via activation of the bile acid receptor TGR5 on enteroendocrine L cells. Metformin too has been shown to increase GLP-1 levels. The effect of gallbladder emptying, metformin or a combination has however never been studied.OBJECTIVE: We hypothesized that cholecystokinin-8 (CCK)-induced gallbladder emptying stimulates human GLP-1 secretion and that metformin would potentiate this effect.DESIGN: A double-blinded, randomized study.SETTING: The study was conducted at a specialized research unit.PARTICIPANTS: Ten healthy male subjects with no family history of diabetes (aged 22 (range 20-32) years; body mass index 21.7 (19.3-24.2) kg/m(2); fasting plasma glucose 4.9 (4.7-5.3) mM; and HbA1c 5.1 (4.4-5.8) %).INTERVENTION: On 4 separate days, the subjects received metformin or placebo and a concomitant 60-minute intravenous infusion of saline or CCK. Blood was sampled for 4 hours and gallbladder volume measured by ultrasound.MAIN OUTCOME MEASURES: Plasma levels of GLP-1.RESULTS: CCK-induced gallbladder emptying and metformin alone (no observed effect on gallbladder emptying), both elicited significant and additive GLP-1 responses. Metformin alone or combined with gallbladder emptying elicited a significant peptide YY response. CCK-induced gallbladder emptying resulted in a short-lasting glucose-dependent insulinotropic polypeptide (GIP) response independent of metformin. No effects were seen on plasma glucose, insulin, C-peptide or gastrin.CONCLUSIONS: CCK-induced gallbladder emptying in healthy subjects elicits significant GLP-1 secretion, which can be potentiated by metformin.

AB - CONTEXT: Bile acids have been suggested to mediate glucagon-like peptide-1 (GLP-1) secretion via activation of the bile acid receptor TGR5 on enteroendocrine L cells. Metformin too has been shown to increase GLP-1 levels. The effect of gallbladder emptying, metformin or a combination has however never been studied.OBJECTIVE: We hypothesized that cholecystokinin-8 (CCK)-induced gallbladder emptying stimulates human GLP-1 secretion and that metformin would potentiate this effect.DESIGN: A double-blinded, randomized study.SETTING: The study was conducted at a specialized research unit.PARTICIPANTS: Ten healthy male subjects with no family history of diabetes (aged 22 (range 20-32) years; body mass index 21.7 (19.3-24.2) kg/m(2); fasting plasma glucose 4.9 (4.7-5.3) mM; and HbA1c 5.1 (4.4-5.8) %).INTERVENTION: On 4 separate days, the subjects received metformin or placebo and a concomitant 60-minute intravenous infusion of saline or CCK. Blood was sampled for 4 hours and gallbladder volume measured by ultrasound.MAIN OUTCOME MEASURES: Plasma levels of GLP-1.RESULTS: CCK-induced gallbladder emptying and metformin alone (no observed effect on gallbladder emptying), both elicited significant and additive GLP-1 responses. Metformin alone or combined with gallbladder emptying elicited a significant peptide YY response. CCK-induced gallbladder emptying resulted in a short-lasting glucose-dependent insulinotropic polypeptide (GIP) response independent of metformin. No effects were seen on plasma glucose, insulin, C-peptide or gastrin.CONCLUSIONS: CCK-induced gallbladder emptying in healthy subjects elicits significant GLP-1 secretion, which can be potentiated by metformin.

U2 - 10.1210/jc.2016-1133

DO - 10.1210/jc.2016-1133

M3 - Journal article

C2 - 27003305

VL - 101

SP - 2076

EP - 2083

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 5

ER -

ID: 160444251