Cell culture plastics with immobilized interleukin-4 for monocyte differentiation
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Cell culture plastics with immobilized interleukin-4 for monocyte differentiation. / Hansen, Morten; Hjortø, Gertrud Malene; Met, Özcan; Jakobsen, Mogens Havsteen; Svane, Inge M; Larsen, Niels.
In: Journal of Biomedical Materials Research. Part A, Vol. 96, No. 2, 2011, p. 372-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cell culture plastics with immobilized interleukin-4 for monocyte differentiation
AU - Hansen, Morten
AU - Hjortø, Gertrud Malene
AU - Met, Özcan
AU - Jakobsen, Mogens Havsteen
AU - Svane, Inge M
AU - Larsen, Niels
N1 - 2010 Wiley Periodicals, Inc.
PY - 2011
Y1 - 2011
N2 - Standard cell culture plastic was surface modified by passive adsorption or covalent attachment of interleukin (IL)-4 and investigated for its ability to induce differentiation of human monocytes into mature dendritic cells, a process dose-dependently regulated by IL-4. Covalent attachment of IL-4 proceeded via anthraquinone photochemistry to introduce amine functionalities at the surface followed by coupling of IL-4 through a bifunctional amine-reactive linker. X-ray photoelectron spectroscopy showed that undesirable multilayer formation of the photoactive compound could be avoided by reaction in water instead of phosphate-buffered saline. Passively adsorbed IL-4 was observed to induce differentiation to dendritic cells, but analysis of cell culture supernatants revealed that leakage of IL-4 into solution could account for the differentiation observed. Covalent attachment resulted in bound IL-4 at similar concentrations to the passive adsorption process, as measured by enzyme-linked immunosorbent assays, and the bound IL-4 did not leak into solution to any measurable extent during cell culture. However, covalently bound IL-4 was incapable of inducing monocyte differentiation. This may be caused by IL-4 denaturation or improper epitope presentation induced by the immobilization process, or by biological irresponsiveness of monocytes to IL-4 in immobilized formats.
AB - Standard cell culture plastic was surface modified by passive adsorption or covalent attachment of interleukin (IL)-4 and investigated for its ability to induce differentiation of human monocytes into mature dendritic cells, a process dose-dependently regulated by IL-4. Covalent attachment of IL-4 proceeded via anthraquinone photochemistry to introduce amine functionalities at the surface followed by coupling of IL-4 through a bifunctional amine-reactive linker. X-ray photoelectron spectroscopy showed that undesirable multilayer formation of the photoactive compound could be avoided by reaction in water instead of phosphate-buffered saline. Passively adsorbed IL-4 was observed to induce differentiation to dendritic cells, but analysis of cell culture supernatants revealed that leakage of IL-4 into solution could account for the differentiation observed. Covalent attachment resulted in bound IL-4 at similar concentrations to the passive adsorption process, as measured by enzyme-linked immunosorbent assays, and the bound IL-4 did not leak into solution to any measurable extent during cell culture. However, covalently bound IL-4 was incapable of inducing monocyte differentiation. This may be caused by IL-4 denaturation or improper epitope presentation induced by the immobilization process, or by biological irresponsiveness of monocytes to IL-4 in immobilized formats.
U2 - http://dx.doi.org/10.1002/jbm.a.32986
DO - http://dx.doi.org/10.1002/jbm.a.32986
M3 - Journal article
VL - 96
SP - 372
EP - 383
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -
ID: 40197053