(19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells

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(19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells. / Malaisse, Willy J; Zhang, Ying; Louchami, Karim; Sharma, Sonu; Dresselaers, Tom; Himmelreich, Uwe; Novotny, Guy Wayne; Mandrup-Poulsen, Thomas; Waschke, Daniel; Leshch, Yevgeniy; Thimm, Julian; Thiem, Joachim; Sener, Abdullah.

In: Archives of Biochemistry and Biophysics, Vol. 517, No. 2, 2012, p. 138-43.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Malaisse, WJ, Zhang, Y, Louchami, K, Sharma, S, Dresselaers, T, Himmelreich, U, Novotny, GW, Mandrup-Poulsen, T, Waschke, D, Leshch, Y, Thimm, J, Thiem, J & Sener, A 2012, '(19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells', Archives of Biochemistry and Biophysics, vol. 517, no. 2, pp. 138-43. https://doi.org/10.1016/j.abb.2011.11.014

APA

Malaisse, W. J., Zhang, Y., Louchami, K., Sharma, S., Dresselaers, T., Himmelreich, U., Novotny, G. W., Mandrup-Poulsen, T., Waschke, D., Leshch, Y., Thimm, J., Thiem, J., & Sener, A. (2012). (19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells. Archives of Biochemistry and Biophysics, 517(2), 138-43. https://doi.org/10.1016/j.abb.2011.11.014

Vancouver

Malaisse WJ, Zhang Y, Louchami K, Sharma S, Dresselaers T, Himmelreich U et al. (19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells. Archives of Biochemistry and Biophysics. 2012;517(2):138-43. https://doi.org/10.1016/j.abb.2011.11.014

Author

Malaisse, Willy J ; Zhang, Ying ; Louchami, Karim ; Sharma, Sonu ; Dresselaers, Tom ; Himmelreich, Uwe ; Novotny, Guy Wayne ; Mandrup-Poulsen, Thomas ; Waschke, Daniel ; Leshch, Yevgeniy ; Thimm, Julian ; Thiem, Joachim ; Sener, Abdullah. / (19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells. In: Archives of Biochemistry and Biophysics. 2012 ; Vol. 517, No. 2. pp. 138-43.

Bibtex

@article{fb5745c271344e279fdda2e28416466a,

title = "(19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells",

abstract = "Suitable analogs of d-mannoheptulose are currently considered as possible tools for the non-invasive imaging of pancreatic islet insulin-producing cells. Here, we examined whether (19)F-heptuloses could be used for non-invasive imaging of GLUT2-expressing cells. After 20 min incubation, the uptake of (19)F-heptuloses (25 mM) by rat hepatocytes, as assessed by (19)F NMR spectroscopy, ranged from 0.50 (1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose) to 0.25 (1,3-dideoxy-1,3-difluoro-d-mannoheptulose) and 0.13 (1-deoxy-1-fluoro-d-glucoheptulose, 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose) µmol per 3×10(6)cells. (19)F MRI experiments also allowed the detection of 1-deoxy-1-fluoro-d-mannoheptulose in rat hepatocytes. All three (19)F-mannoheptuloses cited above, as well as 7-deoxy-7-fluoro-d-mannoheptulose and 1-deoxy-1-fluoro-d-glucoheptulose inhibited insulin release evoked in rat isolated pancreatic islets by 10mM d-glucose to the same extent as that observed with an equivalent concentration (10mM) of d-mannoheptulose, while 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose (also 10mM) were less potent than d-mannoheptulose in inhibiting insulin release. The 1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose only marginally affected INS-1 cell viability. These findings are compatible with the view that selected (19)F-heptuloses may represent suitable tools for the non-invasive imaging of hepatocytes and insulin-producing cells by (19)F MRI.",

keywords = "Animals, Cell Line, Cell Survival, Female, Fluorine, Glucose Transporter Type 2, Hepatocytes, Insulin, Islets of Langerhans, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Mannoheptulose, Phantoms, Imaging, Rats, Rats, Wistar",

author = "Malaisse, {Willy J} and Ying Zhang and Karim Louchami and Sonu Sharma and Tom Dresselaers and Uwe Himmelreich and Novotny, {Guy Wayne} and Thomas Mandrup-Poulsen and Daniel Waschke and Yevgeniy Leshch and Julian Thimm and Joachim Thiem and Abdullah Sener",

year = "2012",

doi = "10.1016/j.abb.2011.11.014",

language = "English",

volume = "517",

pages = "138--43",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press",

number = "2",

}

RIS

TY - JOUR

T1 - (19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells

AU - Malaisse, Willy J

AU - Zhang, Ying

AU - Louchami, Karim

AU - Sharma, Sonu

AU - Dresselaers, Tom

AU - Himmelreich, Uwe

AU - Novotny, Guy Wayne

AU - Mandrup-Poulsen, Thomas

AU - Waschke, Daniel

AU - Leshch, Yevgeniy

AU - Thimm, Julian

AU - Thiem, Joachim

AU - Sener, Abdullah

PY - 2012

Y1 - 2012

N2 - Suitable analogs of d-mannoheptulose are currently considered as possible tools for the non-invasive imaging of pancreatic islet insulin-producing cells. Here, we examined whether (19)F-heptuloses could be used for non-invasive imaging of GLUT2-expressing cells. After 20 min incubation, the uptake of (19)F-heptuloses (25 mM) by rat hepatocytes, as assessed by (19)F NMR spectroscopy, ranged from 0.50 (1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose) to 0.25 (1,3-dideoxy-1,3-difluoro-d-mannoheptulose) and 0.13 (1-deoxy-1-fluoro-d-glucoheptulose, 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose) µmol per 3×10(6)cells. (19)F MRI experiments also allowed the detection of 1-deoxy-1-fluoro-d-mannoheptulose in rat hepatocytes. All three (19)F-mannoheptuloses cited above, as well as 7-deoxy-7-fluoro-d-mannoheptulose and 1-deoxy-1-fluoro-d-glucoheptulose inhibited insulin release evoked in rat isolated pancreatic islets by 10mM d-glucose to the same extent as that observed with an equivalent concentration (10mM) of d-mannoheptulose, while 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose (also 10mM) were less potent than d-mannoheptulose in inhibiting insulin release. The 1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose only marginally affected INS-1 cell viability. These findings are compatible with the view that selected (19)F-heptuloses may represent suitable tools for the non-invasive imaging of hepatocytes and insulin-producing cells by (19)F MRI.

AB - Suitable analogs of d-mannoheptulose are currently considered as possible tools for the non-invasive imaging of pancreatic islet insulin-producing cells. Here, we examined whether (19)F-heptuloses could be used for non-invasive imaging of GLUT2-expressing cells. After 20 min incubation, the uptake of (19)F-heptuloses (25 mM) by rat hepatocytes, as assessed by (19)F NMR spectroscopy, ranged from 0.50 (1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose) to 0.25 (1,3-dideoxy-1,3-difluoro-d-mannoheptulose) and 0.13 (1-deoxy-1-fluoro-d-glucoheptulose, 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose) µmol per 3×10(6)cells. (19)F MRI experiments also allowed the detection of 1-deoxy-1-fluoro-d-mannoheptulose in rat hepatocytes. All three (19)F-mannoheptuloses cited above, as well as 7-deoxy-7-fluoro-d-mannoheptulose and 1-deoxy-1-fluoro-d-glucoheptulose inhibited insulin release evoked in rat isolated pancreatic islets by 10mM d-glucose to the same extent as that observed with an equivalent concentration (10mM) of d-mannoheptulose, while 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose (also 10mM) were less potent than d-mannoheptulose in inhibiting insulin release. The 1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose only marginally affected INS-1 cell viability. These findings are compatible with the view that selected (19)F-heptuloses may represent suitable tools for the non-invasive imaging of hepatocytes and insulin-producing cells by (19)F MRI.

KW - Animals

KW - Cell Line

KW - Cell Survival

KW - Female

KW - Fluorine

KW - Glucose Transporter Type 2

KW - Hepatocytes

KW - Insulin

KW - Islets of Langerhans

KW - Magnetic Resonance Imaging

KW - Magnetic Resonance Spectroscopy

KW - Mannoheptulose

KW - Phantoms, Imaging

KW - Rats

KW - Rats, Wistar

U2 - 10.1016/j.abb.2011.11.014

DO - 10.1016/j.abb.2011.11.014

M3 - Journal article

C2 - 22138222

VL - 517

SP - 138

EP - 143

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -

ID: 38412307

Department of Biomedical Sciences