Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
Originalsprog | Engelsk |
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Titel | P-Type ATPases : Methods and Protocols |
Redaktører | Maike Bublitz |
Antal sider | 8 |
Vol/bind | 1377 |
Forlag | Springer |
Publikationsdato | 2016 |
Sider | 413-420 |
Kapitel | 37 |
ISBN (Trykt) | 978-1-4939-3178-1 |
ISBN (Elektronisk) | 978-1-4939-3179-8 |
DOI | |
Status | Udgivet - 2016 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1377 |
ISSN | 1064-3745 |
ID: 174864931