Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells
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Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells. / Adinolfi, Barbara; Carpi, Sara; Romanini, Antonella; Da Pozzo, Eleonora; Castagna, Maura; Costa, Barbara; Martini, Claudia; Olesen, Søren-Peter; Schmitt, Nicole; Breschi, Maria Cristina; Nieri, Paola; Fogli, Stefano.
I: Anticancer Research, Bind 35, Nr. 7, 07.2015, s. 3781-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Analysis of the Antitumor Activity of Clotrimazole on A375 Human Melanoma Cells
AU - Adinolfi, Barbara
AU - Carpi, Sara
AU - Romanini, Antonella
AU - Da Pozzo, Eleonora
AU - Castagna, Maura
AU - Costa, Barbara
AU - Martini, Claudia
AU - Olesen, Søren-Peter
AU - Schmitt, Nicole
AU - Breschi, Maria Cristina
AU - Nieri, Paola
AU - Fogli, Stefano
N1 - Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
PY - 2015/7
Y1 - 2015/7
N2 - AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.
AB - AIM: The current study was designed to characterize the anticancer effects of clotrimazole on human cutaneous melanoma cells.MATERIALS AND METHODS: The v-raf murine sarcoma viral oncogene homolog B1 V600E mutant melanoma cell line A375 was used as an in vitro model. Characterization tools included analyses of cell viability, gene expression, cell-cycle progression, annexin V reactivity and internucleosomal DNA fragmentation.RESULTS: Clotrimazole induced cytotoxicity in A375 human melanoma cells without significant changes of human keratinocyte cell viability. Clotrimazole, at a concentration that approximates the inhibitory concentration 50% (IC50) value (i.e. 10 μM), reduced the expression of hexokinase type-II, induced cell-cycle arrest at G1-S phase transition, altered annexin V reactivity and induced DNA fragmentation without evidence of necrosis.CONCLUSION: The current study provides evidence of a remarkable pro-apoptotic effect by clotrimazole against human melanoma cells, with a different mechanism of action and timeline of the apoptosis-related events when compared to cisplatin.
KW - Annexin A5
KW - Antineoplastic Agents
KW - Apoptosis
KW - Cell Line, Tumor
KW - Cell Survival
KW - Cisplatin
KW - Clotrimazole
KW - DNA Fragmentation
KW - G1 Phase Cell Cycle Checkpoints
KW - Humans
KW - Keratinocytes
KW - Melanoma
M3 - Journal article
C2 - 26124322
VL - 35
SP - 3781
EP - 3786
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 7
ER -
ID: 149044239