The focus of Associate Professor Bo Hjorth Bentzen’s research group is to understand the mechanism of cardiac arrhythmia and to find new opportunities for treatment of these.
To this end we work with a broad range of model systems ranging from electrophysiological recordings on single cells to isolated perfused hearts, zebrafish and recordings on large animals.
Our work includes investigating the mechanism of inherited cardiac arrhythmia and discovering and validating new ion channel drug target for the treatment of cardiac disorders such Long-QT syndrome, ischemia-reperfusion injuries and atrial fibrillation. To accomplish this we employ different strategies to generate cardiac disease models. We successfully work with models of cardiac ischemia, sophisticated large animal models of atrial fibrillation.
In general our projects focus on understanding the role of ion channels in health and disease. The projects are often performed in collaboration with the other members of the Ion Channel Group who are experts in biochemistry, molecular biology, imaging, and genetics. This ensures that we have all necessary expertise.
Examples of ongoing projects:
- Novel treatments of AF (Target-AF) - A collaborative project between Acesion Pharma and us, funded by the Innovation Fund Denmark
- Transcriptome analysis of atrial fibrillation using bulk and single-nucleus RNA-sequencing
- Epigenetics of AF
- Inherited arrhythmias:
1) Examining the effect of genetic mutations in inherited arrhythmic diseases by patch clamp experiments
- PUFAs and Long-QT syndrome
- Two-electrode voltage-clamp
- Single cell patch clamping
- Automated patch clamping (QPatch) for drug screen on cell cultures and primary cells
- Sharp electrode recordings of action potentials
- Cell proliferation assays
- Primary cell culturing
- Isolated perfused heart preparations
- Optical mapping
- Sophisticated tachypaced models of atrial fibrillation
- Electrophysiology (ECG and effective refractory period measurements)
- Zebrafish recordings