Proinsulin folding and degradation in the Marzec Group

Our focus in the Marzec Group is to understand the mechanisms necessary for efficient proinsulin folding, the role of endoplasmic reticulum chaperones in the process and the relationship to the diabetes pathology.

Our mission is to uncover basic biological processes related to protein folding and harness them to formulate new therapeutic approaches.

We value working environment that combines an ambitious and a social atmosphere with high academic standards, open collaborations and innovation.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Muhammad Saad is employed as PhD student at the Department of Biomedical Sciences, University of Copenhagen. Earlier he earned his bachelors (Doctor of Veterinary Medicine) and Masters (Physiology) from Pakistan.

The focus of Saad’s research is on auto-immune mechanisms related to type-1 diabetes. He will explore the role of GRP94 in processing of pro-insulin. In addition, he will investigate the potential involvement of proteasomes in proinsulin degradation.

Currently Saad is on an exchange visit to Anthony Purcell laboratory at Monash University, Australia.

Carolin Rita Höfner is a research assistant at the Department of Biomedical Sciences, University of Copenhagen funded by the B. Braun foundation and the Bavarian pharmacist association. She graduated from Ludwig-Maximilians University of Munich in 2020 as pharmacist. Recently she did a research internship at the laboratory of Dr. M. Jeske where she worked on transposon silencing.

Carolin is focusing on establishing in vitro enzymatic assays in order to evaluate contribution of different ER proteins (chaperones) to proinsulin folding and processing.

Past lab members

Tenna Holgersen Bryde (M.Sc. in Cell Biology and Physiology, UCPH) was employed as a research assistant at the Department of Biomedical Sciences, University of Copenhagen.

Tenna has been working with Associate Professor Marzec since she joined his group in the fall of 2018, where she finished her Master’s studies exploring proteins co-interacting with GRP94 and proinsulin.

The main objective of Tenna’s work as a research assistant is a continuation of her studies from M.Sc. She will further the research to understand the mechanisms necessary for efficient proinsulin folding and their relationship to diabetes pathology.

Sylvia Naiga Tiedemann (MSc in Human Biology) defended her MSc in Human Biology with a mark 12. She is a co-author of four publications investigating how health care professionals can optimize psoriasis patients’ adherence to topical corticosteroids.

In our lab, Sylvia is now focusing her research on uncovering proinsulin early folding steps. The goal is to give a comprehensive picture of the relationship between ER chaperones and modulating proteins, and to investigate their collaborative effect on insulin biosynthesis and processing.

Celina Phil (Bsc in Molecular Biomedicine) defended her MSc in Molecular Biomedicine with a mark 12. Celina wrote her bachelor’s thesis with Associate Professor Marzec, writing about GRP94, ER stress and degradative pathways.

As a continuation of work previously done in the lab, Celina is now focusing her research on proteins co-interacting with GRP94 and proinsulin. The goal is to give a comprehensive picture of the relationship between ER chaperones and modulating proteins, and to investigate their collaborative effect on insulin biosynthesis and processing.

Sophie Emilie Bresson (Bsc. In Molecular Biomedicine, UCPH) defended MSc in Molecular Biomedicine with a mark 12.

Sophie has been working with the group since January 2018, where she as a bachelor student investigated the interaction between endogenous GRP94 and proinsulin, with focus on identifying novel interaction partners.

Today, Sophie’s work centers around GRP94 and proinsulin variants, investigating their effect on complex formation, protein processing and maturation, ER-stress, and its relation to diabetes.

Mariola Majewska was master student on the ERASMUS exchange in 2021 from Adam Mickiewicz University. She worked on folding similarities within insulin protein family.

Michala Olsen and Elisabeth Frandsen were two bachelor students from Roskilde University that in 2020 evaluated newly generated knockout clonal cell lines and resulting changes in proinsulin processing. Their joint project received a mark of 12.

Piktel Ewelina was an assistant professor on a short ERASMUS exchange from Medical University of Bialystok in 2020. Her main focus was to expand her technical experience and transfer certain techniques and approaches to her laboratory.

Martyna Kosciuszko was an ERASMUS exchange student from Jagiellonian University in 2020 working on proinsulin isomerization project. She is currently a PhD student at the Manchester University.

Christian Kronborg was a medical student on a research internship in 2019 working on proteasome role in proinsulin degradation. His project received a mark 12.

Erika Pinheiro was an ERASMUS exchange student in 2018-2019 from the University of Groningen. She described beta cell secretome in parental and GRP94 deficient INS1-E cells and is a co-author of three publications. She is currently a PhD student at the University of Groningen.

Danielle Verstappen was a master student on a final ERASMUS exchange from Radboud University in 2017-2018. She described a novel proteasome constitutively expressed in pancreatic beta cell and is a co-author on two lab publications.

Caroline Andersen was a master student in 2017-2018 and investigated the relationship between GRP94 activity and proinsulin folding. She graduated with a mark 12.

Solrun Pedersen was a master student in 2015-2017 when she described for the first time the interaction between proinsulin and its chaperone, GRP94. She graduated with a mark 12.

Oana Cheta was a research associate in 2015-2016 performing an initial work on proinsulin folding and playing a critical role in setting our laboratory at the UCPH.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Group leader Michal Marzec

Group Leader
Michal Marzec
Associate Professor

Phone +45 3533 1284
michal@sund.ku.dk