The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels?

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Standard

The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels? / Jespersen, Thomas; Grunnet, Morten; Rasmussen, Hanne B; Jørgensen, Nils B; Jensen, Henrik; Angelo, Kamilla; Olesen, Søren-Peter; Klaerke, Dan A.

In: Biochemical and Biophysical Research Communications, Vol. 341, No. 4, 2006, p. 979-88.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jespersen, T, Grunnet, M, Rasmussen, HB, Jørgensen, NB, Jensen, H, Angelo, K, Olesen, S-P & Klaerke, DA 2006, 'The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels?', Biochemical and Biophysical Research Communications, vol. 341, no. 4, pp. 979-88. https://doi.org/10.1016/j.bbrc.2006.01.046

APA

Jespersen, T., Grunnet, M., Rasmussen, H. B., Jørgensen, N. B., Jensen, H., Angelo, K., Olesen, S-P., & Klaerke, D. A. (2006). The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels? Biochemical and Biophysical Research Communications, 341(4), 979-88. https://doi.org/10.1016/j.bbrc.2006.01.046

Vancouver

Jespersen T, Grunnet M, Rasmussen HB, Jørgensen NB, Jensen H, Angelo K et al. The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels? Biochemical and Biophysical Research Communications. 2006;341(4):979-88. https://doi.org/10.1016/j.bbrc.2006.01.046

Author

Jespersen, Thomas ; Grunnet, Morten ; Rasmussen, Hanne B ; Jørgensen, Nils B ; Jensen, Henrik ; Angelo, Kamilla ; Olesen, Søren-Peter ; Klaerke, Dan A. / The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels?. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 341, No. 4. pp. 979-88.

Bibtex

@article{c884eca0ab5411ddb5e9000ea68e967b,
title = "The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels?",
abstract = "The corticosteroid hormone induced factor (CHIF) is a member of the one-transmembrane segment protein family named FXYD, which also counts phospholemman and the Na,K-pump gamma-subunit. Originally it was suggested that CHIF could induce the expression of the I(Ks) current when expressed in Xenopus laevis oocytes, but recently CHIF has attracted attention as a modulatory subunit of the Na,K-pump. In renal and intestinal epithelia, the expression of CHIF is dramatically up-regulated in response to aldosterone stimulation, and regulation of epithelial ion channels by CHIF is an attractive hypothesis. To study a potential regulatory effect of the CHIF subunit on KCNQ1 channels, co-expression experiments were performed in Xenopus laevis oocytes and mammalian CHO-K1 cells. Electrophysiological characterization was obtained by two-electrode voltage-clamp and patch-clamp, respectively. In both expression systems, we find that CHIF drastically modulates the KCNQ1 current; in the presence of CHIF, the KCNQ1 channels open at all membrane potentials. Thereby, CHIF is the first accessory subunit shown to be capable of modulating both the Na,K-pump and an ion channel. To find a possible physiological function of the constitutively open KCNQ1/CHIF complex, the precise localization of KCNQ1 and CHIF in distal colon and kidney from control and salt-depleted rats was determined by confocal microscopy. However, in these tissues, we did not detect an obvious overlap in expression between KCNQ1 and CHIF. In conclusion, the hormone-regulated subunit CHIF modulates the voltage sensitivity of the KCNQ channels, but so far evidence for an actual co-localization of CHIF and KCNQ1 channels in native tissue is lacking.",
author = "Thomas Jespersen and Morten Grunnet and Rasmussen, {Hanne B} and J{\o}rgensen, {Nils B} and Henrik Jensen and Kamilla Angelo and S{\o}ren-Peter Olesen and Klaerke, {Dan A}",
note = "Keywords: Aldosterone; Animals; Anthracenes; CHO Cells; Colon; Cricetinae; Cricetulus; KCNQ1 Potassium Channel; Kidney; Male; Membrane Potentials; Membrane Proteins; Microscopy, Confocal; Oocytes; Potassium Channels, Voltage-Gated; Rats; Xenopus laevis",
year = "2006",
doi = "10.1016/j.bbrc.2006.01.046",
language = "English",
volume = "341",
pages = "979--88",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - The corticosteroid hormone induced factor: a new modulator of KCNQ1 channels?

AU - Jespersen, Thomas

AU - Grunnet, Morten

AU - Rasmussen, Hanne B

AU - Jørgensen, Nils B

AU - Jensen, Henrik

AU - Angelo, Kamilla

AU - Olesen, Søren-Peter

AU - Klaerke, Dan A

N1 - Keywords: Aldosterone; Animals; Anthracenes; CHO Cells; Colon; Cricetinae; Cricetulus; KCNQ1 Potassium Channel; Kidney; Male; Membrane Potentials; Membrane Proteins; Microscopy, Confocal; Oocytes; Potassium Channels, Voltage-Gated; Rats; Xenopus laevis

PY - 2006

Y1 - 2006

N2 - The corticosteroid hormone induced factor (CHIF) is a member of the one-transmembrane segment protein family named FXYD, which also counts phospholemman and the Na,K-pump gamma-subunit. Originally it was suggested that CHIF could induce the expression of the I(Ks) current when expressed in Xenopus laevis oocytes, but recently CHIF has attracted attention as a modulatory subunit of the Na,K-pump. In renal and intestinal epithelia, the expression of CHIF is dramatically up-regulated in response to aldosterone stimulation, and regulation of epithelial ion channels by CHIF is an attractive hypothesis. To study a potential regulatory effect of the CHIF subunit on KCNQ1 channels, co-expression experiments were performed in Xenopus laevis oocytes and mammalian CHO-K1 cells. Electrophysiological characterization was obtained by two-electrode voltage-clamp and patch-clamp, respectively. In both expression systems, we find that CHIF drastically modulates the KCNQ1 current; in the presence of CHIF, the KCNQ1 channels open at all membrane potentials. Thereby, CHIF is the first accessory subunit shown to be capable of modulating both the Na,K-pump and an ion channel. To find a possible physiological function of the constitutively open KCNQ1/CHIF complex, the precise localization of KCNQ1 and CHIF in distal colon and kidney from control and salt-depleted rats was determined by confocal microscopy. However, in these tissues, we did not detect an obvious overlap in expression between KCNQ1 and CHIF. In conclusion, the hormone-regulated subunit CHIF modulates the voltage sensitivity of the KCNQ channels, but so far evidence for an actual co-localization of CHIF and KCNQ1 channels in native tissue is lacking.

AB - The corticosteroid hormone induced factor (CHIF) is a member of the one-transmembrane segment protein family named FXYD, which also counts phospholemman and the Na,K-pump gamma-subunit. Originally it was suggested that CHIF could induce the expression of the I(Ks) current when expressed in Xenopus laevis oocytes, but recently CHIF has attracted attention as a modulatory subunit of the Na,K-pump. In renal and intestinal epithelia, the expression of CHIF is dramatically up-regulated in response to aldosterone stimulation, and regulation of epithelial ion channels by CHIF is an attractive hypothesis. To study a potential regulatory effect of the CHIF subunit on KCNQ1 channels, co-expression experiments were performed in Xenopus laevis oocytes and mammalian CHO-K1 cells. Electrophysiological characterization was obtained by two-electrode voltage-clamp and patch-clamp, respectively. In both expression systems, we find that CHIF drastically modulates the KCNQ1 current; in the presence of CHIF, the KCNQ1 channels open at all membrane potentials. Thereby, CHIF is the first accessory subunit shown to be capable of modulating both the Na,K-pump and an ion channel. To find a possible physiological function of the constitutively open KCNQ1/CHIF complex, the precise localization of KCNQ1 and CHIF in distal colon and kidney from control and salt-depleted rats was determined by confocal microscopy. However, in these tissues, we did not detect an obvious overlap in expression between KCNQ1 and CHIF. In conclusion, the hormone-regulated subunit CHIF modulates the voltage sensitivity of the KCNQ channels, but so far evidence for an actual co-localization of CHIF and KCNQ1 channels in native tissue is lacking.

U2 - 10.1016/j.bbrc.2006.01.046

DO - 10.1016/j.bbrc.2006.01.046

M3 - Journal article

C2 - 16476578

VL - 341

SP - 979

EP - 988

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -

ID: 8418722