The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo.

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The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo. / Toräng, Signe; Veedfald, Simon; Rosenkilde, Mette Marie; Hartmann, Bolette; Holst, Jens Juul.

In: Physiological Reports, Vol. 3, No. 7, e12455, 20.07.2015, p. 1-8.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Toräng, S, Veedfald, S, Rosenkilde, MM, Hartmann, B & Holst, JJ 2015, 'The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo.', Physiological Reports, vol. 3, no. 7, e12455, pp. 1-8. https://doi.org/10.14814/phy2.12455

APA

Toräng, S., Veedfald, S., Rosenkilde, M. M., Hartmann, B., & Holst, J. J. (2015). The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo. Physiological Reports, 3(7), 1-8. [e12455]. https://doi.org/10.14814/phy2.12455

Vancouver

Toräng S, Veedfald S, Rosenkilde MM, Hartmann B, Holst JJ. The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo. Physiological Reports. 2015 Jul 20;3(7):1-8. e12455. https://doi.org/10.14814/phy2.12455

Author

Toräng, Signe ; Veedfald, Simon ; Rosenkilde, Mette Marie ; Hartmann, Bolette ; Holst, Jens Juul. / The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo. In: Physiological Reports. 2015 ; Vol. 3, No. 7. pp. 1-8.

Bibtex

@article{85cf3182a8144d6390c64ad3b34e484c,
title = "The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo.",
abstract = "Peptide YY (PYY) is a 36 amino acid peptide hormone released from enteroendocrine cells. An N-terminally degraded metabolite, PYY3-36, has anorexigenic effects, which makes the PYY system a target for obesity treatment. However, little is known about the kinetics and degrdn. products of PYY. A related peptide, Neuropeptide Y (NPY), may be degraded from the C-terminus. We therefore investigated PYY degrdn. after in vitro incubations in porcine plasma and blood and in vivo by infusing PYY3-36 into multicatheterized pigs (n = 7) (2 pmol/kg/min). Plasma samples were analyzed by region-specific RIAs (RIA) and HPLC anal. A metabolite, corresponding to PYY3-34 was formed after incubation in plasma and blood and during the infusion study. When taking the C-terminal degrdn. into account, the half-life (T1/2) of PYY in blood and plasma amounted to 3.4 ± 0.2 and 6.2 ± 0.2 h, resp. After PYY3-36 infusion in pigs, the peptide was degraded with a T1/2 of 3.6 ± 0.5 min. Significant extn. (20.5 ± 8.0{\%}) compatible with glomerular filtration was obsd. across the kidneys and significant C-terminal degrdn. (26.5 ± 4.8{\%}) was obsd. across the liver. Net balances across the hind limb, splanchnic bed, and lungs were not significantly different from zero. PYY3-34 was unable to activate the Y2 receptor in a transfected cell line. In conclusion, PYY3-36 is extensively degraded to PYY3-34 in the pig, a degrdn. that renders the peptide inactive on the Y2 receptor. Currently used assays are unlikely to be able to detect this degrdn. and therefore measure falsely elevated levels of PYY3-36, leading to underestimation of its physiol. effects.",
author = "Signe Tor{\"a}ng and Simon Veedfald and Rosenkilde, {Mette Marie} and Bolette Hartmann and Holst, {Jens Juul}",
note = "M1 - Copyright (C) 2015 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2015:1289224(Journal; Online Computer File)",
year = "2015",
month = "7",
day = "20",
doi = "10.14814/phy2.12455",
language = "English",
volume = "3",
pages = "1--8",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "Wiley Periodicals, Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo.

AU - Toräng, Signe

AU - Veedfald, Simon

AU - Rosenkilde, Mette Marie

AU - Hartmann, Bolette

AU - Holst, Jens Juul

N1 - M1 - Copyright (C) 2015 American Chemical Society (ACS). All Rights Reserved. CAPLUS AN 2015:1289224(Journal; Online Computer File)

PY - 2015/7/20

Y1 - 2015/7/20

N2 - Peptide YY (PYY) is a 36 amino acid peptide hormone released from enteroendocrine cells. An N-terminally degraded metabolite, PYY3-36, has anorexigenic effects, which makes the PYY system a target for obesity treatment. However, little is known about the kinetics and degrdn. products of PYY. A related peptide, Neuropeptide Y (NPY), may be degraded from the C-terminus. We therefore investigated PYY degrdn. after in vitro incubations in porcine plasma and blood and in vivo by infusing PYY3-36 into multicatheterized pigs (n = 7) (2 pmol/kg/min). Plasma samples were analyzed by region-specific RIAs (RIA) and HPLC anal. A metabolite, corresponding to PYY3-34 was formed after incubation in plasma and blood and during the infusion study. When taking the C-terminal degrdn. into account, the half-life (T1/2) of PYY in blood and plasma amounted to 3.4 ± 0.2 and 6.2 ± 0.2 h, resp. After PYY3-36 infusion in pigs, the peptide was degraded with a T1/2 of 3.6 ± 0.5 min. Significant extn. (20.5 ± 8.0%) compatible with glomerular filtration was obsd. across the kidneys and significant C-terminal degrdn. (26.5 ± 4.8%) was obsd. across the liver. Net balances across the hind limb, splanchnic bed, and lungs were not significantly different from zero. PYY3-34 was unable to activate the Y2 receptor in a transfected cell line. In conclusion, PYY3-36 is extensively degraded to PYY3-34 in the pig, a degrdn. that renders the peptide inactive on the Y2 receptor. Currently used assays are unlikely to be able to detect this degrdn. and therefore measure falsely elevated levels of PYY3-36, leading to underestimation of its physiol. effects.

AB - Peptide YY (PYY) is a 36 amino acid peptide hormone released from enteroendocrine cells. An N-terminally degraded metabolite, PYY3-36, has anorexigenic effects, which makes the PYY system a target for obesity treatment. However, little is known about the kinetics and degrdn. products of PYY. A related peptide, Neuropeptide Y (NPY), may be degraded from the C-terminus. We therefore investigated PYY degrdn. after in vitro incubations in porcine plasma and blood and in vivo by infusing PYY3-36 into multicatheterized pigs (n = 7) (2 pmol/kg/min). Plasma samples were analyzed by region-specific RIAs (RIA) and HPLC anal. A metabolite, corresponding to PYY3-34 was formed after incubation in plasma and blood and during the infusion study. When taking the C-terminal degrdn. into account, the half-life (T1/2) of PYY in blood and plasma amounted to 3.4 ± 0.2 and 6.2 ± 0.2 h, resp. After PYY3-36 infusion in pigs, the peptide was degraded with a T1/2 of 3.6 ± 0.5 min. Significant extn. (20.5 ± 8.0%) compatible with glomerular filtration was obsd. across the kidneys and significant C-terminal degrdn. (26.5 ± 4.8%) was obsd. across the liver. Net balances across the hind limb, splanchnic bed, and lungs were not significantly different from zero. PYY3-34 was unable to activate the Y2 receptor in a transfected cell line. In conclusion, PYY3-36 is extensively degraded to PYY3-34 in the pig, a degrdn. that renders the peptide inactive on the Y2 receptor. Currently used assays are unlikely to be able to detect this degrdn. and therefore measure falsely elevated levels of PYY3-36, leading to underestimation of its physiol. effects.

U2 - 10.14814/phy2.12455

DO - 10.14814/phy2.12455

M3 - Journal article

VL - 3

SP - 1

EP - 8

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 7

M1 - e12455

ER -

ID: 150701053