Superoxide radicals have a protective role during H2O2 stress
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Superoxide radicals have a protective role during H2O2 stress. / Thorpe, Geoffrey W; Reodica, Mayfebelle; Davies, Michael Jonathan; Heeren, Gino; Jarolim, Stefanie; Pillay, Bethany; Breitenbach, Michael; Higgins, Vincent J; Dawes, Ian W.
In: Molecular Biology of the Cell, Vol. 24, No. 18, 09.2013, p. 2876-84.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Superoxide radicals have a protective role during H2O2 stress
AU - Thorpe, Geoffrey W
AU - Reodica, Mayfebelle
AU - Davies, Michael Jonathan
AU - Heeren, Gino
AU - Jarolim, Stefanie
AU - Pillay, Bethany
AU - Breitenbach, Michael
AU - Higgins, Vincent J
AU - Dawes, Ian W
PY - 2013/9
Y1 - 2013/9
N2 - Reactive oxygen species (ROS) consist of potentially toxic, partly reduced oxygen species and free radicals. After H(2)O(2) treatment, yeast cells significantly increase superoxide radical production. Respiratory chain complex III and possibly cytochrome b function are essential for this increase. Disruption of complex III renders cells sensitive to H(2)O(2) but not to the superoxide radical generator menadione. Of interest, the same H(2)O(2)-sensitive mutant strains have the lowest superoxide radical levels, and strains with the highest resistance to H(2)O(2) have the highest levels of superoxide radicals. Consistent with this correlation, overexpression of superoxide dismutase increases sensitivity to H(2)O(2), and this phenotype is partially rescued by addition of small concentrations of menadione. Small increases in levels of mitochondrially produced superoxide radicals have a protective effect during H(2)O(2)-induced stress, and in response to H(2)O(2), the wild-type strain increases superoxide radical production to activate this defense mechanism. This provides a direct link between complex III as the main source of ROS and its role in defense against ROS. High levels of the superoxide radical are still toxic. These opposing, concentration-dependent roles of the superoxide radical comprise a form of hormesis and show one ROS having a hormetic effect on the toxicity of another.
AB - Reactive oxygen species (ROS) consist of potentially toxic, partly reduced oxygen species and free radicals. After H(2)O(2) treatment, yeast cells significantly increase superoxide radical production. Respiratory chain complex III and possibly cytochrome b function are essential for this increase. Disruption of complex III renders cells sensitive to H(2)O(2) but not to the superoxide radical generator menadione. Of interest, the same H(2)O(2)-sensitive mutant strains have the lowest superoxide radical levels, and strains with the highest resistance to H(2)O(2) have the highest levels of superoxide radicals. Consistent with this correlation, overexpression of superoxide dismutase increases sensitivity to H(2)O(2), and this phenotype is partially rescued by addition of small concentrations of menadione. Small increases in levels of mitochondrially produced superoxide radicals have a protective effect during H(2)O(2)-induced stress, and in response to H(2)O(2), the wild-type strain increases superoxide radical production to activate this defense mechanism. This provides a direct link between complex III as the main source of ROS and its role in defense against ROS. High levels of the superoxide radical are still toxic. These opposing, concentration-dependent roles of the superoxide radical comprise a form of hormesis and show one ROS having a hormetic effect on the toxicity of another.
KW - Adaptation, Physiological
KW - Cytochromes b
KW - Cytoprotection
KW - Electron Transport
KW - Gene Expression Regulation, Enzymologic
KW - Hydrogen Peroxide
KW - Microbial Viability
KW - Mitochondria
KW - Mutation
KW - Oxidative Stress
KW - RNA, Messenger
KW - Saccharomyces cerevisiae
KW - Stress, Physiological
KW - Superoxide Dismutase
KW - Superoxides
U2 - 10.1091/mbc.E13-01-0052
DO - 10.1091/mbc.E13-01-0052
M3 - Journal article
C2 - 23864711
VL - 24
SP - 2876
EP - 2884
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 18
ER -
ID: 128974215