Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart.

Research output: Contribution to journalJournal articlepeer-review

Standard

Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart. / Rasmussen, Hanne Borger; Møller, Morten; Knaus, Hans-Günther; Jensen, Bo Skaaning; Olesen, Søren-Peter; Jørgensen, Nanna Koschmieder.

In: American Journal of Physiology: Heart and Circulatory Physiology, Vol. 286, No. 4, 2003, p. H1300-9.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Rasmussen, HB, Møller, M, Knaus, H-G, Jensen, BS, Olesen, S-P & Jørgensen, NK 2003, 'Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart.', American Journal of Physiology: Heart and Circulatory Physiology, vol. 286, no. 4, pp. H1300-9. https://doi.org/10.1152/ajpheart.00344.2003

APA

Rasmussen, H. B., Møller, M., Knaus, H-G., Jensen, B. S., Olesen, S-P., & Jørgensen, N. K. (2003). Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart. American Journal of Physiology: Heart and Circulatory Physiology, 286(4), H1300-9. https://doi.org/10.1152/ajpheart.00344.2003

Vancouver

Rasmussen HB, Møller M, Knaus H-G, Jensen BS, Olesen S-P, Jørgensen NK. Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart. American Journal of Physiology: Heart and Circulatory Physiology. 2003;286(4):H1300-9. https://doi.org/10.1152/ajpheart.00344.2003

Author

Rasmussen, Hanne Borger ; Møller, Morten ; Knaus, Hans-Günther ; Jensen, Bo Skaaning ; Olesen, Søren-Peter ; Jørgensen, Nanna Koschmieder. / Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart. In: American Journal of Physiology: Heart and Circulatory Physiology. 2003 ; Vol. 286, No. 4. pp. H1300-9.

Bibtex

@article{16539c70ac1211ddb5e9000ea68e967b,
title = "Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart.",
abstract = "In the heart, several K(+) channels are responsible for the repolarization of the cardiac action potential, including transient outward and delayed rectifier K(+) currents. In the present study, the cellular and subcellular localization of the two delayed rectifier K(+) channels, KCNQ1 and ether-a-go-go-related gene-1 (ERG1), was investigated in the adult rat heart. Confocal immunofluorescence microscopy of atrial and ventricular cells revealed that whereas KCNQ1 labeling was detected in both the peripheral sarcolemma and a structure transversing the myocytes, ERG1 immunoreactivity was confined to the latter. Immunoelectron microscopy of atrial and ventricular myocytes showed that the ERG1 channel was primarily expressed in the transverse tubular system and its entrance, whereas KCNQ1 was detected in both the peripheral sarcolemma and in the T tubules. Thus, whereas ERG1 displays a very restricted subcellular localization pattern, KCNQ1 is more widely distributed within the cardiac cells. The localization of these K(+) channels to the transverse tubular system close to the Ca(2+) channels renders them with maximal repolarizing effect.",
author = "Rasmussen, {Hanne Borger} and Morten M{\o}ller and Hans-G{\"u}nther Knaus and Jensen, {Bo Skaaning} and S{\o}ren-Peter Olesen and J{\o}rgensen, {Nanna Koschmieder}",
note = "Keywords: Action Potentials; Animals; Antibodies, Blocking; Blotting, Western; Ether-A-Go-Go Potassium Channels; Immunohistochemistry; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Microscopy, Confocal; Microscopy, Immunoelectron; Myocardium; Myocytes, Cardiac; Potassium Channel Blockers; Potassium Channels; Potassium Channels, Voltage-Gated; Rats; Sarcolemma; Subcellular Fractions",
year = "2003",
doi = "10.1152/ajpheart.00344.2003",
language = "English",
volume = "286",
pages = "H1300--9",
journal = "American Journal of Physiology: Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart.

AU - Rasmussen, Hanne Borger

AU - Møller, Morten

AU - Knaus, Hans-Günther

AU - Jensen, Bo Skaaning

AU - Olesen, Søren-Peter

AU - Jørgensen, Nanna Koschmieder

N1 - Keywords: Action Potentials; Animals; Antibodies, Blocking; Blotting, Western; Ether-A-Go-Go Potassium Channels; Immunohistochemistry; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Microscopy, Confocal; Microscopy, Immunoelectron; Myocardium; Myocytes, Cardiac; Potassium Channel Blockers; Potassium Channels; Potassium Channels, Voltage-Gated; Rats; Sarcolemma; Subcellular Fractions

PY - 2003

Y1 - 2003

N2 - In the heart, several K(+) channels are responsible for the repolarization of the cardiac action potential, including transient outward and delayed rectifier K(+) currents. In the present study, the cellular and subcellular localization of the two delayed rectifier K(+) channels, KCNQ1 and ether-a-go-go-related gene-1 (ERG1), was investigated in the adult rat heart. Confocal immunofluorescence microscopy of atrial and ventricular cells revealed that whereas KCNQ1 labeling was detected in both the peripheral sarcolemma and a structure transversing the myocytes, ERG1 immunoreactivity was confined to the latter. Immunoelectron microscopy of atrial and ventricular myocytes showed that the ERG1 channel was primarily expressed in the transverse tubular system and its entrance, whereas KCNQ1 was detected in both the peripheral sarcolemma and in the T tubules. Thus, whereas ERG1 displays a very restricted subcellular localization pattern, KCNQ1 is more widely distributed within the cardiac cells. The localization of these K(+) channels to the transverse tubular system close to the Ca(2+) channels renders them with maximal repolarizing effect.

AB - In the heart, several K(+) channels are responsible for the repolarization of the cardiac action potential, including transient outward and delayed rectifier K(+) currents. In the present study, the cellular and subcellular localization of the two delayed rectifier K(+) channels, KCNQ1 and ether-a-go-go-related gene-1 (ERG1), was investigated in the adult rat heart. Confocal immunofluorescence microscopy of atrial and ventricular cells revealed that whereas KCNQ1 labeling was detected in both the peripheral sarcolemma and a structure transversing the myocytes, ERG1 immunoreactivity was confined to the latter. Immunoelectron microscopy of atrial and ventricular myocytes showed that the ERG1 channel was primarily expressed in the transverse tubular system and its entrance, whereas KCNQ1 was detected in both the peripheral sarcolemma and in the T tubules. Thus, whereas ERG1 displays a very restricted subcellular localization pattern, KCNQ1 is more widely distributed within the cardiac cells. The localization of these K(+) channels to the transverse tubular system close to the Ca(2+) channels renders them with maximal repolarizing effect.

U2 - 10.1152/ajpheart.00344.2003

DO - 10.1152/ajpheart.00344.2003

M3 - Journal article

C2 - 14670813

VL - 286

SP - H1300-9

JO - American Journal of Physiology: Heart and Circulatory Physiology

JF - American Journal of Physiology: Heart and Circulatory Physiology

SN - 0363-6135

IS - 4

ER -

ID: 8443812