Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins. / Adav, Sunil S.; Subbaiaih, Roopa S.; Kerk, Swat Kim; Lee, Amelia Yilin; Lai, HuiYing; Ng, Kee Woei; Sze, Siu Kwan; Schmidtchen, Artur.
In: Scientific Reports, Vol. 8, 1599 , 2018.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins
AU - Adav, Sunil S.
AU - Subbaiaih, Roopa S.
AU - Kerk, Swat Kim
AU - Lee, Amelia Yilin
AU - Lai, HuiYing
AU - Ng, Kee Woei
AU - Sze, Siu Kwan
AU - Schmidtchen, Artur
PY - 2018
Y1 - 2018
N2 - Human hair is laminar-fibrous tissue and an evolutionarily old keratinization product of follicle trichocytes. Studies on the hair proteome can give new insights into hair function and lead to the development of novel biomarkers for hair in health and disease. Human hair proteins were extracted by detergent and detergent-free techniques. We adopted a shotgun proteomics approach, which demonstrated a large extractability and variety of hair proteins after detergent extraction. We found an enrichment of keratin, keratin-associated proteins (KAPs), and intermediate filament proteins, which were part of protein networks associated with response to stress, innate immunity, epidermis development, and the hair cycle. Our analysis also revealed a significant deamidation of keratin type I and II, and KAPs. The hair shafts were found to contain several types of histones, which are well known to exert antimicrobial activity. Analysis of the hair proteome, particularly its composition, protein abundances, deamidated hair proteins, and modification sites, may offer a novel approach to explore potential biomarkers of hair health quality, hair diseases, and aging.
AB - Human hair is laminar-fibrous tissue and an evolutionarily old keratinization product of follicle trichocytes. Studies on the hair proteome can give new insights into hair function and lead to the development of novel biomarkers for hair in health and disease. Human hair proteins were extracted by detergent and detergent-free techniques. We adopted a shotgun proteomics approach, which demonstrated a large extractability and variety of hair proteins after detergent extraction. We found an enrichment of keratin, keratin-associated proteins (KAPs), and intermediate filament proteins, which were part of protein networks associated with response to stress, innate immunity, epidermis development, and the hair cycle. Our analysis also revealed a significant deamidation of keratin type I and II, and KAPs. The hair shafts were found to contain several types of histones, which are well known to exert antimicrobial activity. Analysis of the hair proteome, particularly its composition, protein abundances, deamidated hair proteins, and modification sites, may offer a novel approach to explore potential biomarkers of hair health quality, hair diseases, and aging.
U2 - 10.1038/s41598-018-20041-9
DO - 10.1038/s41598-018-20041-9
M3 - Journal article
C2 - 29371649
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 1599
ER -
ID: 216163199