Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons

Research output: Contribution to journalJournal articleResearchpeer-review

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Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons. / Jensen, Camilla Stampe; Watanabe, Shoji; Rasmussen, Hanne Borger; Schmitt, Nicole; Olesen, Søren-Peter; Frost, Nicholas A; Blanpied, Thomas A; Misonou, Hiroaki.

In: The Journal of Biological Chemistry, Vol. 289, 25.02.2014, p. 10566-10581.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jensen, CS, Watanabe, S, Rasmussen, HB, Schmitt, N, Olesen, S-P, Frost, NA, Blanpied, TA & Misonou, H 2014, 'Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons', The Journal of Biological Chemistry, vol. 289, pp. 10566-10581. https://doi.org/10.1074/jbc.M113.534495

APA

Jensen, C. S., Watanabe, S., Rasmussen, H. B., Schmitt, N., Olesen, S-P., Frost, N. A., ... Misonou, H. (2014). Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons. The Journal of Biological Chemistry, 289, 10566-10581. https://doi.org/10.1074/jbc.M113.534495

Vancouver

Jensen CS, Watanabe S, Rasmussen HB, Schmitt N, Olesen S-P, Frost NA et al. Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons. The Journal of Biological Chemistry. 2014 Feb 25;289:10566-10581. https://doi.org/10.1074/jbc.M113.534495

Author

Jensen, Camilla Stampe ; Watanabe, Shoji ; Rasmussen, Hanne Borger ; Schmitt, Nicole ; Olesen, Søren-Peter ; Frost, Nicholas A ; Blanpied, Thomas A ; Misonou, Hiroaki. / Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons. In: The Journal of Biological Chemistry. 2014 ; Vol. 289. pp. 10566-10581.

Bibtex

@article{357393706acd4c99b9e0790531050ea6,
title = "Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons",
abstract = "Proper membrane localization of ion channels is essential for the function of neuronal cells. Particularly, the computational ability of dendrites depends on the localization of different ion channels in specific sub-compartments. However, the molecular mechanisms which control ion channel localization in distinct dendritic sub-compartments are largely unknown. Here, we developed a quantitative live-cell imaging method to analyze protein sorting and post-Golgi vesicular trafficking. We focused on two dendritic voltage-gated potassium channels which exhibit distinct localizations; Kv2.1 in proximal dendrites and Kv4.2 in distal dendrites. Our results show that Kv2.1 and Kv4.2 channels are sorted into two distinct populations of vesicles at the Golgi apparatus. The targeting of Kv2.1 and Kv4.2 vesicles occurred by distinct mechanisms evidenced by their requirement for specific peptide motifs, cytoskeletal elements, and motor proteins. By live-cell and super-resolution imaging, we identified a novel trafficking machinery important for the localization of Kv2.1 channels. Particularly, we identified non-muscle myosin II as an important factor in Kv2.1 trafficking. These findings reveal that the sorting of ion channels at the Golgi apparatus and their subsequent trafficking by unique molecular mechanisms, are crucial for their specific localizations within dendrites.",
author = "Jensen, {Camilla Stampe} and Shoji Watanabe and Rasmussen, {Hanne Borger} and Nicole Schmitt and S{\o}ren-Peter Olesen and Frost, {Nicholas A} and Blanpied, {Thomas A} and Hiroaki Misonou",
year = "2014",
month = "2",
day = "25",
doi = "10.1074/jbc.M113.534495",
language = "English",
volume = "289",
pages = "10566--10581",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

}

RIS

TY - JOUR

T1 - Specific Sorting and Post-Golgi trafficking of Dendritic Potassium Channels in Living Neurons

AU - Jensen, Camilla Stampe

AU - Watanabe, Shoji

AU - Rasmussen, Hanne Borger

AU - Schmitt, Nicole

AU - Olesen, Søren-Peter

AU - Frost, Nicholas A

AU - Blanpied, Thomas A

AU - Misonou, Hiroaki

PY - 2014/2/25

Y1 - 2014/2/25

N2 - Proper membrane localization of ion channels is essential for the function of neuronal cells. Particularly, the computational ability of dendrites depends on the localization of different ion channels in specific sub-compartments. However, the molecular mechanisms which control ion channel localization in distinct dendritic sub-compartments are largely unknown. Here, we developed a quantitative live-cell imaging method to analyze protein sorting and post-Golgi vesicular trafficking. We focused on two dendritic voltage-gated potassium channels which exhibit distinct localizations; Kv2.1 in proximal dendrites and Kv4.2 in distal dendrites. Our results show that Kv2.1 and Kv4.2 channels are sorted into two distinct populations of vesicles at the Golgi apparatus. The targeting of Kv2.1 and Kv4.2 vesicles occurred by distinct mechanisms evidenced by their requirement for specific peptide motifs, cytoskeletal elements, and motor proteins. By live-cell and super-resolution imaging, we identified a novel trafficking machinery important for the localization of Kv2.1 channels. Particularly, we identified non-muscle myosin II as an important factor in Kv2.1 trafficking. These findings reveal that the sorting of ion channels at the Golgi apparatus and their subsequent trafficking by unique molecular mechanisms, are crucial for their specific localizations within dendrites.

AB - Proper membrane localization of ion channels is essential for the function of neuronal cells. Particularly, the computational ability of dendrites depends on the localization of different ion channels in specific sub-compartments. However, the molecular mechanisms which control ion channel localization in distinct dendritic sub-compartments are largely unknown. Here, we developed a quantitative live-cell imaging method to analyze protein sorting and post-Golgi vesicular trafficking. We focused on two dendritic voltage-gated potassium channels which exhibit distinct localizations; Kv2.1 in proximal dendrites and Kv4.2 in distal dendrites. Our results show that Kv2.1 and Kv4.2 channels are sorted into two distinct populations of vesicles at the Golgi apparatus. The targeting of Kv2.1 and Kv4.2 vesicles occurred by distinct mechanisms evidenced by their requirement for specific peptide motifs, cytoskeletal elements, and motor proteins. By live-cell and super-resolution imaging, we identified a novel trafficking machinery important for the localization of Kv2.1 channels. Particularly, we identified non-muscle myosin II as an important factor in Kv2.1 trafficking. These findings reveal that the sorting of ion channels at the Golgi apparatus and their subsequent trafficking by unique molecular mechanisms, are crucial for their specific localizations within dendrites.

U2 - 10.1074/jbc.M113.534495

DO - 10.1074/jbc.M113.534495

M3 - Journal article

VL - 289

SP - 10566

EP - 10581

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

ER -

ID: 102671918