Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution

Research output: Working paperPreprintResearch

Standard

Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution. / Martinez Del Val, Ana; Bekker-Jensen, Dorte B; Steigerwald, Sophia; Mehta, Adi; Tran, Trung; Sikorski, Krzysztof; Torres Vega, Estefania; Kwasniewicz, Ewa; Brynjólfsdóttir, Sólveig Hlín; Frankel, Lisa; Kjøbsted, Rasmus; Krogh, Nicolai; Lundby, Alicia; Bekker-Jensen, Simon; Lund-Johansen, Fridtjof; Olsen, Jesper Velgaard.

bioRxiv, 2021. p. 1-34.

Research output: Working paperPreprintResearch

Harvard

Martinez Del Val, A, Bekker-Jensen, DB, Steigerwald, S, Mehta, A, Tran, T, Sikorski, K, Torres Vega, E, Kwasniewicz, E, Brynjólfsdóttir, SH, Frankel, L, Kjøbsted, R, Krogh, N, Lundby, A, Bekker-Jensen, S, Lund-Johansen, F & Olsen, JV 2021 'Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution' bioRxiv, pp. 1-34. https://doi.org/10.1101/2021.02.02.425898

APA

Martinez Del Val, A., Bekker-Jensen, D. B., Steigerwald, S., Mehta, A., Tran, T., Sikorski, K., Torres Vega, E., Kwasniewicz, E., Brynjólfsdóttir, S. H., Frankel, L., Kjøbsted, R., Krogh, N., Lundby, A., Bekker-Jensen, S., Lund-Johansen, F., & Olsen, J. V. (2021). Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution. (pp. 1-34). bioRxiv. https://doi.org/10.1101/2021.02.02.425898

Vancouver

Martinez Del Val A, Bekker-Jensen DB, Steigerwald S, Mehta A, Tran T, Sikorski K et al. Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution. bioRxiv. 2021 Feb 2, p. 1-34. https://doi.org/10.1101/2021.02.02.425898

Author

Martinez Del Val, Ana ; Bekker-Jensen, Dorte B ; Steigerwald, Sophia ; Mehta, Adi ; Tran, Trung ; Sikorski, Krzysztof ; Torres Vega, Estefania ; Kwasniewicz, Ewa ; Brynjólfsdóttir, Sólveig Hlín ; Frankel, Lisa ; Kjøbsted, Rasmus ; Krogh, Nicolai ; Lundby, Alicia ; Bekker-Jensen, Simon ; Lund-Johansen, Fridtjof ; Olsen, Jesper Velgaard. / Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution. bioRxiv, 2021. pp. 1-34

Bibtex

@techreport{76a8a067cf1d4676bda58672dec231ca,
title = "Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution",
abstract = "Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmarked the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro in HeLa cells and in-vivo in mouse tissues. Finally, we investigated the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io.",
author = "{Martinez Del Val}, Ana and Bekker-Jensen, {Dorte B} and Sophia Steigerwald and Adi Mehta and Trung Tran and Krzysztof Sikorski and {Torres Vega}, Estefania and Ewa Kwasniewicz and Brynj{\'o}lfsd{\'o}ttir, {S{\'o}lveig Hl{\'i}n} and Lisa Frankel and Rasmus Kj{\o}bsted and Nicolai Krogh and Alicia Lundby and Simon Bekker-Jensen and Fridtjof Lund-Johansen and Olsen, {Jesper Velgaard}",
note = "(Preprint)",
year = "2021",
month = feb,
day = "2",
doi = "10.1101/2021.02.02.425898",
language = "English",
pages = "1--34",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution

AU - Martinez Del Val, Ana

AU - Bekker-Jensen, Dorte B

AU - Steigerwald, Sophia

AU - Mehta, Adi

AU - Tran, Trung

AU - Sikorski, Krzysztof

AU - Torres Vega, Estefania

AU - Kwasniewicz, Ewa

AU - Brynjólfsdóttir, Sólveig Hlín

AU - Frankel, Lisa

AU - Kjøbsted, Rasmus

AU - Krogh, Nicolai

AU - Lundby, Alicia

AU - Bekker-Jensen, Simon

AU - Lund-Johansen, Fridtjof

AU - Olsen, Jesper Velgaard

N1 - (Preprint)

PY - 2021/2/2

Y1 - 2021/2/2

N2 - Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmarked the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro in HeLa cells and in-vivo in mouse tissues. Finally, we investigated the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io.

AB - Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmarked the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro in HeLa cells and in-vivo in mouse tissues. Finally, we investigated the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io.

U2 - 10.1101/2021.02.02.425898

DO - 10.1101/2021.02.02.425898

M3 - Preprint

SP - 1

EP - 34

BT - Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution

PB - bioRxiv

ER -

ID: 328735289