TY - JOUR

T1 - Skeletal Muscle Mitochondrial Function in Polycystic Ovarian Syndrome

AU - Rabøl, Rasmus

AU - Svendsen, Pernille Maj

AU - Skovbro, Mette

AU - Boushel, Robert Christopher

AU - Schjerling, Peter

AU - Nilas, Lisbeth

AU - Madsbad, Sten

AU - Dela, Flemming

PY - 2011

Y1 - 2011

N2 - Objective Polycystic ovarian syndrome (PCOS) is associated with skeletal muscle insulin resistance, which has been linked to decreased mitochondrial function. We measured mitochondrial respiration in lean and obese women with and without PCOS using high-resolution respirometry. Methods Hyperinsulinemic euglycemic clamps (40 mU/min/m2) and muscle biopsies were performed on 23 women with PCOS (9 lean (body mass index (BMI) 25 kg/m2)) and 17 age- and weight-matched controls (6 lean and 11 obese). Western blotting and high-resolution respirometry was used to determine mitochondrial function. Results Insulin sensitivity decreased with PCOS and increasing body weight. Mitochondrial respiration with substrates for complex I and complex I+II were similar in all groups, and PCOS was not associated with a decrease in mitochondrial content as measured by mtDNA/genomicDNA. We found no correlation between mitochondrial function and indices of insulin sensitivity. Conclusions In contrast to previous reports we found no evidence that skeletal muscle mitochondrial respiration is reduced in skeletal muscle of women with PCOS compared to control subjects. Furthermore, mitochondrial content did not differ between our control and PCOS groups. These results question the causal relationship between reduced mitochondrial function and skeletal muscle insulin resistance in PCOS.

AB - Objective Polycystic ovarian syndrome (PCOS) is associated with skeletal muscle insulin resistance, which has been linked to decreased mitochondrial function. We measured mitochondrial respiration in lean and obese women with and without PCOS using high-resolution respirometry. Methods Hyperinsulinemic euglycemic clamps (40 mU/min/m2) and muscle biopsies were performed on 23 women with PCOS (9 lean (body mass index (BMI) 25 kg/m2)) and 17 age- and weight-matched controls (6 lean and 11 obese). Western blotting and high-resolution respirometry was used to determine mitochondrial function. Results Insulin sensitivity decreased with PCOS and increasing body weight. Mitochondrial respiration with substrates for complex I and complex I+II were similar in all groups, and PCOS was not associated with a decrease in mitochondrial content as measured by mtDNA/genomicDNA. We found no correlation between mitochondrial function and indices of insulin sensitivity. Conclusions In contrast to previous reports we found no evidence that skeletal muscle mitochondrial respiration is reduced in skeletal muscle of women with PCOS compared to control subjects. Furthermore, mitochondrial content did not differ between our control and PCOS groups. These results question the causal relationship between reduced mitochondrial function and skeletal muscle insulin resistance in PCOS.

U2 - 10.1530/EJE-11-0419

DO - 10.1530/EJE-11-0419

M3 - Journal article

C2 - 21798960

VL - 165

SP - 631

EP - 637

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

SN - 0804-4643

IS - 4

ER -