Potential targets for glucagon-like peptide 2 (GLP-2) in the rat: distribution and binding of i.v. injected (125)I-GLP-2

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Potential targets for glucagon-like peptide 2 (GLP-2) in the rat : distribution and binding of i.v. injected (125)I-GLP-2. / Thulesen, J; Hartmann, B; Orskov, C; Jeppesen, P B; Holst, J J; Poulsen, Steen Seier.

In: Peptides, Vol. 21, No. 10, 10.2000, p. 1511-7.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Thulesen, J, Hartmann, B, Orskov, C, Jeppesen, PB, Holst, JJ & Poulsen, SS 2000, 'Potential targets for glucagon-like peptide 2 (GLP-2) in the rat: distribution and binding of i.v. injected (125)I-GLP-2', Peptides, vol. 21, no. 10, pp. 1511-7.

APA

Thulesen, J., Hartmann, B., Orskov, C., Jeppesen, P. B., Holst, J. J., & Poulsen, S. S. (2000). Potential targets for glucagon-like peptide 2 (GLP-2) in the rat: distribution and binding of i.v. injected (125)I-GLP-2. Peptides, 21(10), 1511-7.

Vancouver

Thulesen J, Hartmann B, Orskov C, Jeppesen PB, Holst JJ, Poulsen SS. Potential targets for glucagon-like peptide 2 (GLP-2) in the rat: distribution and binding of i.v. injected (125)I-GLP-2. Peptides. 2000 Oct;21(10):1511-7.

Author

Thulesen, J ; Hartmann, B ; Orskov, C ; Jeppesen, P B ; Holst, J J ; Poulsen, Steen Seier. / Potential targets for glucagon-like peptide 2 (GLP-2) in the rat : distribution and binding of i.v. injected (125)I-GLP-2. In: Peptides. 2000 ; Vol. 21, No. 10. pp. 1511-7.

Bibtex

@article{ee5b195e06ed4f649c9732f81c000373,
title = "Potential targets for glucagon-like peptide 2 (GLP-2) in the rat: distribution and binding of i.v. injected (125)I-GLP-2",
abstract = "Glucagon-like peptide 2 (GLP-2) is a 33-amino acid (1-33) intestinotrophic peptide. In this study, the distribution and binding of i.v. injected radiolabeled GLP-2 (1-33) were investigated in rats using autoradiography in order to target possible binding sites. The major part of (125)I-GLP-2 (1-33) was distributed to kidneys, liver, and the gastrointestinal tract. In the small intestine, a high density of grains was localized in the epithelium with a predominance in the luminal part of the villus. The saturability of (125)I-GLP-2 (1-33) was investigated by administration of excess amounts of non-radioactive GLP-2 (1-33) or the primary metabolite of GLP-2 degradation, GLP-2 (3-33). In the small intestine, (125)I-GLP-2 was displaced both by non-radioactive GLP-2 (1-33) and (3-33), suggesting that the uptake of GLP-2 (1-33) in the small intestine is receptor-specific and that the metabolite GLP-2 (3-33) may interact with the GLP-2 receptor.",
keywords = "Animals, Autoradiography, Binding, Competitive, Epithelium, Female, Glucagon-Like Peptide 2, Glucagon-Like Peptides, Injections, Intravenous, Intestine, Small, Iodine Radioisotopes, Kidney, Liver, Peptides, Protein Binding, Radioimmunoassay, Rats, Rats, Wistar, Receptors, Glucagon, Substrate Specificity",
author = "J Thulesen and B Hartmann and C Orskov and Jeppesen, {P B} and Holst, {J J} and Poulsen, {Steen Seier}",
year = "2000",
month = "10",
language = "English",
volume = "21",
pages = "1511--7",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Potential targets for glucagon-like peptide 2 (GLP-2) in the rat

T2 - distribution and binding of i.v. injected (125)I-GLP-2

AU - Thulesen, J

AU - Hartmann, B

AU - Orskov, C

AU - Jeppesen, P B

AU - Holst, J J

AU - Poulsen, Steen Seier

PY - 2000/10

Y1 - 2000/10

N2 - Glucagon-like peptide 2 (GLP-2) is a 33-amino acid (1-33) intestinotrophic peptide. In this study, the distribution and binding of i.v. injected radiolabeled GLP-2 (1-33) were investigated in rats using autoradiography in order to target possible binding sites. The major part of (125)I-GLP-2 (1-33) was distributed to kidneys, liver, and the gastrointestinal tract. In the small intestine, a high density of grains was localized in the epithelium with a predominance in the luminal part of the villus. The saturability of (125)I-GLP-2 (1-33) was investigated by administration of excess amounts of non-radioactive GLP-2 (1-33) or the primary metabolite of GLP-2 degradation, GLP-2 (3-33). In the small intestine, (125)I-GLP-2 was displaced both by non-radioactive GLP-2 (1-33) and (3-33), suggesting that the uptake of GLP-2 (1-33) in the small intestine is receptor-specific and that the metabolite GLP-2 (3-33) may interact with the GLP-2 receptor.

AB - Glucagon-like peptide 2 (GLP-2) is a 33-amino acid (1-33) intestinotrophic peptide. In this study, the distribution and binding of i.v. injected radiolabeled GLP-2 (1-33) were investigated in rats using autoradiography in order to target possible binding sites. The major part of (125)I-GLP-2 (1-33) was distributed to kidneys, liver, and the gastrointestinal tract. In the small intestine, a high density of grains was localized in the epithelium with a predominance in the luminal part of the villus. The saturability of (125)I-GLP-2 (1-33) was investigated by administration of excess amounts of non-radioactive GLP-2 (1-33) or the primary metabolite of GLP-2 degradation, GLP-2 (3-33). In the small intestine, (125)I-GLP-2 was displaced both by non-radioactive GLP-2 (1-33) and (3-33), suggesting that the uptake of GLP-2 (1-33) in the small intestine is receptor-specific and that the metabolite GLP-2 (3-33) may interact with the GLP-2 receptor.

KW - Animals

KW - Autoradiography

KW - Binding, Competitive

KW - Epithelium

KW - Female

KW - Glucagon-Like Peptide 2

KW - Glucagon-Like Peptides

KW - Injections, Intravenous

KW - Intestine, Small

KW - Iodine Radioisotopes

KW - Kidney

KW - Liver

KW - Peptides

KW - Protein Binding

KW - Radioimmunoassay

KW - Rats

KW - Rats, Wistar

KW - Receptors, Glucagon

KW - Substrate Specificity

M3 - Journal article

C2 - 11068098

VL - 21

SP - 1511

EP - 1517

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 10

ER -

ID: 47486360