Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets. / Niinikoski, Harri; Stoll, Barbara; Guan, Xinfu; Kansagra, Ketan; Lambert, Barry D; Stephens, John; Hartmann, Bolette; Holst, Jens Juul; Burrin, Douglas G.

In: Journal of Nutrition, Vol. 134, No. 6, 06.2004, p. 1467-74.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Niinikoski, H, Stoll, B, Guan, X, Kansagra, K, Lambert, BD, Stephens, J, Hartmann, B, Holst, JJ & Burrin, DG 2004, 'Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets', Journal of Nutrition, vol. 134, no. 6, pp. 1467-74.

APA

Niinikoski, H., Stoll, B., Guan, X., Kansagra, K., Lambert, B. D., Stephens, J., Hartmann, B., Holst, J. J., & Burrin, D. G. (2004). Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets. Journal of Nutrition, 134(6), 1467-74.

Vancouver

Niinikoski H, Stoll B, Guan X, Kansagra K, Lambert BD, Stephens J et al. Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets. Journal of Nutrition. 2004 Jun;134(6):1467-74.

Author

Niinikoski, Harri ; Stoll, Barbara ; Guan, Xinfu ; Kansagra, Ketan ; Lambert, Barry D ; Stephens, John ; Hartmann, Bolette ; Holst, Jens Juul ; Burrin, Douglas G. / Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets. In: Journal of Nutrition. 2004 ; Vol. 134, No. 6. pp. 1467-74.

Bibtex

@article{f1b1c6f5f92145db994a519fc5f1f47a,
title = "Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets",
abstract = "Our aim was to determine the speed of onset of total parenteral nutrition (TPN)-induced mucosal atrophy, and whether this is associated with changes in intestinal blood flow and tissue metabolism in neonatal piglets. Piglets were implanted with jugular venous and duodenal catheters and either a portal venous or superior mesenteric artery (SMA) blood flow probe. At 3 wk of age, piglets were randomly assigned to receive continuous enteral formula feeding (n = 8) or TPN (n = 17) for 24 or 48 h. Blood flow was recorded continuously and piglets were given an i.v. bolus of bromodeoxyuridine and (13)C-phenylalanine to measure crypt cell proliferation and protein synthesis, respectively. After 8 h of TPN, portal and SMA blood flow decreased 30% compared with enteral feeding (P < 0.01), and remained near levels of food-deprived piglets for the remaining 48 h of TPN. After 24 h, TPN reduced jejunal inducible nitric oxide synthase (iNOS) activity and protein abundance (P < 0.05), small intestinal weight, and villous height (P < 0.01) compared with enterally fed piglets. Cell proliferation and DNA mass were decreased (P < 0.05) and apoptosis increased (P < 0.05) after 48 h of TPN. Protein synthesis was lower (P < 0.05) after 24 h of TPN, and protein mass was lower (P < 0.05) after 48 h of TPN, compared with enteral feeding. These data indicate that the transition from enteral to parenteral nutrition induced a rapid (<8 h) decrease in intestinal blood flow, and this likely precedes villous atrophy and the suppression of protein synthesis at 24 h, and of cell proliferation and survival at 48 h.",
keywords = "Animals, Animals, Newborn, Apoptosis, Atrophy, Cell Division, DNA, Enteral Nutrition, Female, Food Deprivation, Glucagon-Like Peptides, Intestinal Mucosa, Intestine, Small, Nitric Oxide Synthase, Organ Size, Parenteral Nutrition, Total, Peptides, Portal System, Protein Biosynthesis, Regional Blood Flow, Survival Rate, Swine, Time Factors",
author = "Harri Niinikoski and Barbara Stoll and Xinfu Guan and Ketan Kansagra and Lambert, {Barry D} and John Stephens and Bolette Hartmann and Holst, {Jens Juul} and Burrin, {Douglas G}",
year = "2004",
month = jun,
language = "English",
volume = "134",
pages = "1467--74",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "6",

}

RIS

TY - JOUR

T1 - Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets

AU - Niinikoski, Harri

AU - Stoll, Barbara

AU - Guan, Xinfu

AU - Kansagra, Ketan

AU - Lambert, Barry D

AU - Stephens, John

AU - Hartmann, Bolette

AU - Holst, Jens Juul

AU - Burrin, Douglas G

PY - 2004/6

Y1 - 2004/6

N2 - Our aim was to determine the speed of onset of total parenteral nutrition (TPN)-induced mucosal atrophy, and whether this is associated with changes in intestinal blood flow and tissue metabolism in neonatal piglets. Piglets were implanted with jugular venous and duodenal catheters and either a portal venous or superior mesenteric artery (SMA) blood flow probe. At 3 wk of age, piglets were randomly assigned to receive continuous enteral formula feeding (n = 8) or TPN (n = 17) for 24 or 48 h. Blood flow was recorded continuously and piglets were given an i.v. bolus of bromodeoxyuridine and (13)C-phenylalanine to measure crypt cell proliferation and protein synthesis, respectively. After 8 h of TPN, portal and SMA blood flow decreased 30% compared with enteral feeding (P < 0.01), and remained near levels of food-deprived piglets for the remaining 48 h of TPN. After 24 h, TPN reduced jejunal inducible nitric oxide synthase (iNOS) activity and protein abundance (P < 0.05), small intestinal weight, and villous height (P < 0.01) compared with enterally fed piglets. Cell proliferation and DNA mass were decreased (P < 0.05) and apoptosis increased (P < 0.05) after 48 h of TPN. Protein synthesis was lower (P < 0.05) after 24 h of TPN, and protein mass was lower (P < 0.05) after 48 h of TPN, compared with enteral feeding. These data indicate that the transition from enteral to parenteral nutrition induced a rapid (<8 h) decrease in intestinal blood flow, and this likely precedes villous atrophy and the suppression of protein synthesis at 24 h, and of cell proliferation and survival at 48 h.

AB - Our aim was to determine the speed of onset of total parenteral nutrition (TPN)-induced mucosal atrophy, and whether this is associated with changes in intestinal blood flow and tissue metabolism in neonatal piglets. Piglets were implanted with jugular venous and duodenal catheters and either a portal venous or superior mesenteric artery (SMA) blood flow probe. At 3 wk of age, piglets were randomly assigned to receive continuous enteral formula feeding (n = 8) or TPN (n = 17) for 24 or 48 h. Blood flow was recorded continuously and piglets were given an i.v. bolus of bromodeoxyuridine and (13)C-phenylalanine to measure crypt cell proliferation and protein synthesis, respectively. After 8 h of TPN, portal and SMA blood flow decreased 30% compared with enteral feeding (P < 0.01), and remained near levels of food-deprived piglets for the remaining 48 h of TPN. After 24 h, TPN reduced jejunal inducible nitric oxide synthase (iNOS) activity and protein abundance (P < 0.05), small intestinal weight, and villous height (P < 0.01) compared with enterally fed piglets. Cell proliferation and DNA mass were decreased (P < 0.05) and apoptosis increased (P < 0.05) after 48 h of TPN. Protein synthesis was lower (P < 0.05) after 24 h of TPN, and protein mass was lower (P < 0.05) after 48 h of TPN, compared with enteral feeding. These data indicate that the transition from enteral to parenteral nutrition induced a rapid (<8 h) decrease in intestinal blood flow, and this likely precedes villous atrophy and the suppression of protein synthesis at 24 h, and of cell proliferation and survival at 48 h.

KW - Animals

KW - Animals, Newborn

KW - Apoptosis

KW - Atrophy

KW - Cell Division

KW - DNA

KW - Enteral Nutrition

KW - Female

KW - Food Deprivation

KW - Glucagon-Like Peptides

KW - Intestinal Mucosa

KW - Intestine, Small

KW - Nitric Oxide Synthase

KW - Organ Size

KW - Parenteral Nutrition, Total

KW - Peptides

KW - Portal System

KW - Protein Biosynthesis

KW - Regional Blood Flow

KW - Survival Rate

KW - Swine

KW - Time Factors

M3 - Journal article

C2 - 15173413

VL - 134

SP - 1467

EP - 1474

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 6

ER -

ID: 132054370