Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector

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Neodymium-140 DOTA-LM3 : Evaluation of anGenerator for PET with a Non-Internalizing Vector. / Severin, Gregory W; Kristensen, Lotte K; Nielsen, Carsten H; Fonslet, Jesper; Jensen, Andreas I; Frellsen, Anders F; Jensen, K M; Elema, Dennis R; Maecke, Helmut; Kjær, Andreas; Johnston, Karl; Köster, Ulli.

In: Frontiers in Medicine, Vol. 4, 98, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Severin, GW, Kristensen, LK, Nielsen, CH, Fonslet, J, Jensen, AI, Frellsen, AF, Jensen, KM, Elema, DR, Maecke, H, Kjær, A, Johnston, K & Köster, U 2017, 'Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector', Frontiers in Medicine, vol. 4, 98. https://doi.org/10.3389/fmed.2017.00098

APA

Severin, G. W., Kristensen, L. K., Nielsen, C. H., Fonslet, J., Jensen, A. I., Frellsen, A. F., ... Köster, U. (2017). Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector. Frontiers in Medicine, 4, [98]. https://doi.org/10.3389/fmed.2017.00098

Vancouver

Severin GW, Kristensen LK, Nielsen CH, Fonslet J, Jensen AI, Frellsen AF et al. Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector. Frontiers in Medicine. 2017;4. 98. https://doi.org/10.3389/fmed.2017.00098

Author

Severin, Gregory W ; Kristensen, Lotte K ; Nielsen, Carsten H ; Fonslet, Jesper ; Jensen, Andreas I ; Frellsen, Anders F ; Jensen, K M ; Elema, Dennis R ; Maecke, Helmut ; Kjær, Andreas ; Johnston, Karl ; Köster, Ulli. / Neodymium-140 DOTA-LM3 : Evaluation of anGenerator for PET with a Non-Internalizing Vector. In: Frontiers in Medicine. 2017 ; Vol. 4.

Bibtex

@article{30e4920c723a4c4188613dc43ae1556f,
title = "Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector",
abstract = "140 Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter140Pr (t1/2 = 3.4 min), has promise as anin vivogenerator for positron emission tomography (PET). However, the electron capture decay of140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that anin vivoredistribution of the daughter140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled140Pr affects preclinical imaging with140Nd. To explore the effect,140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify thein vivoredistribution of140Pr following140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of140Nd-DOTA-LM3 (13{\%} ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7{\%} (n = 4, mean ± SD) of thein situproduced140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free140Pr3+. Based upon these results, we conclude that140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior.",
author = "Severin, {Gregory W} and Kristensen, {Lotte K} and Nielsen, {Carsten H} and Jesper Fonslet and Jensen, {Andreas I} and Frellsen, {Anders F} and Jensen, {K M} and Elema, {Dennis R} and Helmut Maecke and Andreas Kj{\ae}r and Karl Johnston and Ulli K{\"o}ster",
year = "2017",
doi = "10.3389/fmed.2017.00098",
language = "English",
volume = "4",
journal = "Frontiers in Medicine",
issn = "2296-858X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Neodymium-140 DOTA-LM3

T2 - Evaluation of anGenerator for PET with a Non-Internalizing Vector

AU - Severin, Gregory W

AU - Kristensen, Lotte K

AU - Nielsen, Carsten H

AU - Fonslet, Jesper

AU - Jensen, Andreas I

AU - Frellsen, Anders F

AU - Jensen, K M

AU - Elema, Dennis R

AU - Maecke, Helmut

AU - Kjær, Andreas

AU - Johnston, Karl

AU - Köster, Ulli

PY - 2017

Y1 - 2017

N2 - 140 Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter140Pr (t1/2 = 3.4 min), has promise as anin vivogenerator for positron emission tomography (PET). However, the electron capture decay of140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that anin vivoredistribution of the daughter140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled140Pr affects preclinical imaging with140Nd. To explore the effect,140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify thein vivoredistribution of140Pr following140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of140Nd-DOTA-LM3 (13% ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7% (n = 4, mean ± SD) of thein situproduced140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free140Pr3+. Based upon these results, we conclude that140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior.

AB - 140 Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter140Pr (t1/2 = 3.4 min), has promise as anin vivogenerator for positron emission tomography (PET). However, the electron capture decay of140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that anin vivoredistribution of the daughter140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled140Pr affects preclinical imaging with140Nd. To explore the effect,140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify thein vivoredistribution of140Pr following140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of140Nd-DOTA-LM3 (13% ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7% (n = 4, mean ± SD) of thein situproduced140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free140Pr3+. Based upon these results, we conclude that140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior.

U2 - 10.3389/fmed.2017.00098

DO - 10.3389/fmed.2017.00098

M3 - Journal article

C2 - 28748183

VL - 4

JO - Frontiers in Medicine

JF - Frontiers in Medicine

SN - 2296-858X

M1 - 98

ER -

ID: 194529489