Multimodal Positron Emission Tomography Imaging to Quantify Uptake of 89Zr-Labeled Liposomes in the Atherosclerotic Vessel Wall
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Multimodal Positron Emission Tomography Imaging to Quantify Uptake of 89Zr-Labeled Liposomes in the Atherosclerotic Vessel Wall. / Lobatto, Mark E; Binderup, Tina; Robson, Philip M; Giesen, Luuk F P; Calcagno, Claudia; Witjes, Julia; Fay, Francois; Baxter, Samantha; Wessel, Chang Ho; Eldib, Mootaz; Bini, Jason; Carlin, Sean D; Stroes, Erik S G; Storm, Gert; Kjaer, Andreas; Lewis, Jason S; Reiner, Thomas; Fayad, Zahi A; Mulder, Willem J M; Pérez-Medina, Carlos.
In: Bioconjugate Chemistry, Vol. 31, No. 2, 19.02.2020, p. 360-368.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Multimodal Positron Emission Tomography Imaging to Quantify Uptake of 89Zr-Labeled Liposomes in the Atherosclerotic Vessel Wall
AU - Lobatto, Mark E
AU - Binderup, Tina
AU - Robson, Philip M
AU - Giesen, Luuk F P
AU - Calcagno, Claudia
AU - Witjes, Julia
AU - Fay, Francois
AU - Baxter, Samantha
AU - Wessel, Chang Ho
AU - Eldib, Mootaz
AU - Bini, Jason
AU - Carlin, Sean D
AU - Stroes, Erik S G
AU - Storm, Gert
AU - Kjaer, Andreas
AU - Lewis, Jason S
AU - Reiner, Thomas
AU - Fayad, Zahi A
AU - Mulder, Willem J M
AU - Pérez-Medina, Carlos
PY - 2020/2/19
Y1 - 2020/2/19
N2 - Nanotherapy has recently emerged as an experimental treatment option for atherosclerosis. To fulfill its promise, robust noninvasive imaging approaches for subject selection and treatment evaluation are warranted. To that end, we present here a positron emission tomography (PET)-based method for quantification of liposomal nanoparticle uptake in the atherosclerotic vessel wall. We evaluated a modular procedure to label liposomal nanoparticles with the radioisotope zirconium-89 (89Zr). Their biodistribution and vessel wall targeting in a rabbit atherosclerosis model was evaluated up to 15 days after intravenous injection by PET/computed tomography (CT) and PET/magnetic resonance imaging (PET/MRI). Vascular permeability was assessed in vivo using three-dimensional dynamic contrast-enhanced MRI (3D DCE-MRI) and ex vivo using near-infrared fluorescence (NIRF) imaging. The 89Zr-radiolabeled liposomes displayed a biodistribution pattern typical of long-circulating nanoparticles. Importantly, they markedly accumulated in atherosclerotic lesions in the abdominal aorta, as evident on PET/MRI and confirmed by autoradiography, and this uptake moderately correlated with vascular permeability. The method presented herein facilitates the development of nanotherapy for atherosclerotic disease as it provides a tool to screen for nanoparticle targeting in individual subjects' plaques.
AB - Nanotherapy has recently emerged as an experimental treatment option for atherosclerosis. To fulfill its promise, robust noninvasive imaging approaches for subject selection and treatment evaluation are warranted. To that end, we present here a positron emission tomography (PET)-based method for quantification of liposomal nanoparticle uptake in the atherosclerotic vessel wall. We evaluated a modular procedure to label liposomal nanoparticles with the radioisotope zirconium-89 (89Zr). Their biodistribution and vessel wall targeting in a rabbit atherosclerosis model was evaluated up to 15 days after intravenous injection by PET/computed tomography (CT) and PET/magnetic resonance imaging (PET/MRI). Vascular permeability was assessed in vivo using three-dimensional dynamic contrast-enhanced MRI (3D DCE-MRI) and ex vivo using near-infrared fluorescence (NIRF) imaging. The 89Zr-radiolabeled liposomes displayed a biodistribution pattern typical of long-circulating nanoparticles. Importantly, they markedly accumulated in atherosclerotic lesions in the abdominal aorta, as evident on PET/MRI and confirmed by autoradiography, and this uptake moderately correlated with vascular permeability. The method presented herein facilitates the development of nanotherapy for atherosclerotic disease as it provides a tool to screen for nanoparticle targeting in individual subjects' plaques.
U2 - 10.1021/acs.bioconjchem.9b00256
DO - 10.1021/acs.bioconjchem.9b00256
M3 - Journal article
C2 - 31095372
VL - 31
SP - 360
EP - 368
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 2
ER -
ID: 243151953