Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles

Research output: Contribution to journalJournal articleResearch

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Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles. / Jepps, Thomas Andrew; Greenwood, Iain A; Moffatt, James D; Sanders, Kenton M; Ohya, Susumu.

In: A J P: Gastrointestinal and Liver Physiology (Online), Vol. 297, No. 1, 07.2009, p. G107-15.

Research output: Contribution to journalJournal articleResearch

Harvard

Jepps, TA, Greenwood, IA, Moffatt, JD, Sanders, KM & Ohya, S 2009, 'Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles', A J P: Gastrointestinal and Liver Physiology (Online), vol. 297, no. 1, pp. G107-15. https://doi.org/10.1152/ajpgi.00057.2009

APA

Jepps, T. A., Greenwood, I. A., Moffatt, J. D., Sanders, K. M., & Ohya, S. (2009). Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles. A J P: Gastrointestinal and Liver Physiology (Online), 297(1), G107-15. https://doi.org/10.1152/ajpgi.00057.2009

Vancouver

Jepps TA, Greenwood IA, Moffatt JD, Sanders KM, Ohya S. Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles. A J P: Gastrointestinal and Liver Physiology (Online). 2009 Jul;297(1):G107-15. https://doi.org/10.1152/ajpgi.00057.2009

Author

Jepps, Thomas Andrew ; Greenwood, Iain A ; Moffatt, James D ; Sanders, Kenton M ; Ohya, Susumu. / Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles. In: A J P: Gastrointestinal and Liver Physiology (Online). 2009 ; Vol. 297, No. 1. pp. G107-15.

Bibtex

@article{010bcd1671a046e39ab6855879588c21,
title = "Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles",
abstract = "Members of the K(v)7 voltage-gated K(+) channel family are important determinants of cardiac and neuronal membrane excitability. Recently, we and others have shown that K(v)7 channels are also crucial regulators of smooth muscle activity. The aim of the present study was to assess the K(v)7 expression in different parts of the murine gastrointestinal (GI) tract and to assess their functional roles by use of pharmacological agents. Of KCNQ/K(v)7 members, both KCNQ4/K(v)7.4 and KCNQ5/K(v)7.5 genes and proteins were the most abundantly expressed K(v)7 channels in smooth muscles throughout the GI tract. Immunohistochemical staining also revealed that K(v)7.4 and K(v)7.5 but not K(v)7.1 were expressed in the circular muscle layer of the colon. In segments of distal colon circular muscle exhibiting spontaneous phasic contractions, the nonselective K(v)7 blockers XE991 and linopirdine increased the integral of tension. Increases in the integral of tension were also observed under conditions of neuronal blockade. Similar effects, although less marked, were observed in the proximal colon. As expected, the K(v)7.1-selective blocker chromanol 293B had no effect in either type of segment. These data show that K(v)7.x especially K(v)7.4 and K(v)7.5 are expressed in different regions of the murine gastrointestinal tract and blockers of K(v)7 channels augment inherent contractile activity. Drugs that selectively block K(v)7.4/7.5 might be promising therapeutics for the treatment of motility disorders such as constipation associated with irritable bowel syndrome.",
keywords = "Animals, Anthracenes, Blotting, Western, Carbamates, Chromans, Colon, Gastrointestinal Tract, Immunohistochemistry, Indoles, KCNQ Potassium Channels, KCNQ1 Potassium Channel, Mice, Mice, Inbred BALB C, Muscle Contraction, Muscle, Smooth, Myography, Phenylenediamines, Potassium Channel Blockers, Pyridines, Reverse Transcriptase Polymerase Chain Reaction, Sulfonamides",
author = "Jepps, {Thomas Andrew} and Greenwood, {Iain A} and Moffatt, {James D} and Sanders, {Kenton M} and Susumu Ohya",
year = "2009",
month = "7",
doi = "10.1152/ajpgi.00057.2009",
language = "English",
volume = "297",
pages = "G107--15",
journal = "A J P: Gastrointestinal and Liver Physiology (Online)",
issn = "1522-1547",
publisher = "American Physiological Society",
number = "1",

}

RIS

TY - JOUR

T1 - Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles

AU - Jepps, Thomas Andrew

AU - Greenwood, Iain A

AU - Moffatt, James D

AU - Sanders, Kenton M

AU - Ohya, Susumu

PY - 2009/7

Y1 - 2009/7

N2 - Members of the K(v)7 voltage-gated K(+) channel family are important determinants of cardiac and neuronal membrane excitability. Recently, we and others have shown that K(v)7 channels are also crucial regulators of smooth muscle activity. The aim of the present study was to assess the K(v)7 expression in different parts of the murine gastrointestinal (GI) tract and to assess their functional roles by use of pharmacological agents. Of KCNQ/K(v)7 members, both KCNQ4/K(v)7.4 and KCNQ5/K(v)7.5 genes and proteins were the most abundantly expressed K(v)7 channels in smooth muscles throughout the GI tract. Immunohistochemical staining also revealed that K(v)7.4 and K(v)7.5 but not K(v)7.1 were expressed in the circular muscle layer of the colon. In segments of distal colon circular muscle exhibiting spontaneous phasic contractions, the nonselective K(v)7 blockers XE991 and linopirdine increased the integral of tension. Increases in the integral of tension were also observed under conditions of neuronal blockade. Similar effects, although less marked, were observed in the proximal colon. As expected, the K(v)7.1-selective blocker chromanol 293B had no effect in either type of segment. These data show that K(v)7.x especially K(v)7.4 and K(v)7.5 are expressed in different regions of the murine gastrointestinal tract and blockers of K(v)7 channels augment inherent contractile activity. Drugs that selectively block K(v)7.4/7.5 might be promising therapeutics for the treatment of motility disorders such as constipation associated with irritable bowel syndrome.

AB - Members of the K(v)7 voltage-gated K(+) channel family are important determinants of cardiac and neuronal membrane excitability. Recently, we and others have shown that K(v)7 channels are also crucial regulators of smooth muscle activity. The aim of the present study was to assess the K(v)7 expression in different parts of the murine gastrointestinal (GI) tract and to assess their functional roles by use of pharmacological agents. Of KCNQ/K(v)7 members, both KCNQ4/K(v)7.4 and KCNQ5/K(v)7.5 genes and proteins were the most abundantly expressed K(v)7 channels in smooth muscles throughout the GI tract. Immunohistochemical staining also revealed that K(v)7.4 and K(v)7.5 but not K(v)7.1 were expressed in the circular muscle layer of the colon. In segments of distal colon circular muscle exhibiting spontaneous phasic contractions, the nonselective K(v)7 blockers XE991 and linopirdine increased the integral of tension. Increases in the integral of tension were also observed under conditions of neuronal blockade. Similar effects, although less marked, were observed in the proximal colon. As expected, the K(v)7.1-selective blocker chromanol 293B had no effect in either type of segment. These data show that K(v)7.x especially K(v)7.4 and K(v)7.5 are expressed in different regions of the murine gastrointestinal tract and blockers of K(v)7 channels augment inherent contractile activity. Drugs that selectively block K(v)7.4/7.5 might be promising therapeutics for the treatment of motility disorders such as constipation associated with irritable bowel syndrome.

KW - Animals

KW - Anthracenes

KW - Blotting, Western

KW - Carbamates

KW - Chromans

KW - Colon

KW - Gastrointestinal Tract

KW - Immunohistochemistry

KW - Indoles

KW - KCNQ Potassium Channels

KW - KCNQ1 Potassium Channel

KW - Mice

KW - Mice, Inbred BALB C

KW - Muscle Contraction

KW - Muscle, Smooth

KW - Myography

KW - Phenylenediamines

KW - Potassium Channel Blockers

KW - Pyridines

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sulfonamides

U2 - 10.1152/ajpgi.00057.2009

DO - 10.1152/ajpgi.00057.2009

M3 - Journal article

C2 - 19389803

VL - 297

SP - G107-15

JO - A J P: Gastrointestinal and Liver Physiology (Online)

JF - A J P: Gastrointestinal and Liver Physiology (Online)

SN - 1522-1547

IS - 1

ER -

ID: 108539609