Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV.

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Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV. / Hansen, Lene; Hare, Kristine J; Hartmann, Bolette; Deacon, Carolyn F; Ugleholdt, Randi K; Plamboeck, Astrid; Holst, Jens J.

In: Regulatory Peptides, Vol. 138, No. 2-3, 2006, p. 126-32.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hansen, L, Hare, KJ, Hartmann, B, Deacon, CF, Ugleholdt, RK, Plamboeck, A & Holst, JJ 2006, 'Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV.', Regulatory Peptides, vol. 138, no. 2-3, pp. 126-32. https://doi.org/10.1016/j.regpep.2006.08.012

APA

Hansen, L., Hare, K. J., Hartmann, B., Deacon, C. F., Ugleholdt, R. K., Plamboeck, A., & Holst, J. J. (2006). Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV. Regulatory Peptides, 138(2-3), 126-32. https://doi.org/10.1016/j.regpep.2006.08.012

Vancouver

Hansen L, Hare KJ, Hartmann B, Deacon CF, Ugleholdt RK, Plamboeck A et al. Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV. Regulatory Peptides. 2006;138(2-3):126-32. https://doi.org/10.1016/j.regpep.2006.08.012

Author

Hansen, Lene ; Hare, Kristine J ; Hartmann, Bolette ; Deacon, Carolyn F ; Ugleholdt, Randi K ; Plamboeck, Astrid ; Holst, Jens J. / Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV. In: Regulatory Peptides. 2006 ; Vol. 138, No. 2-3. pp. 126-32.

Bibtex

@article{c43a99c0ab4911ddb5e9000ea68e967b,
title = "Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV.",
abstract = "Little is known about the metabolism of the intestinotropic factor glucagon-like peptide-2 (GLP-2); except that it is a substrate for dipeptidyl peptidase IV (DPP-IV) and that it appears to be eliminated by the kidneys. We, therefore, investigated GLP-2 metabolism in six multicatheterized pigs receiving intravenous GLP-2 infusions (2 pmol/kg/min) before and after administration of valine-pyrrolidide (300 mumol/kg; a well characterized DPP-IV inhibitor). Plasma samples were analyzed by radioimmunoassays allowing determination of intact, biologically active GLP-2 and the DPP-IV metabolite GLP-2 (3-33). During infusion of GLP-2 alone, 30.9+/-1.7{\%} of the infused peptide was degraded to GLP-2 (3-33). After valine-pyrrolidide, there was no significant formation of the metabolite. Significant extraction of intact GLP-2 was observed across the kidneys, the extremities (represented by a leg), and the splanchnic bed, resulting in a metabolic clearance rate (MCR) of 6.80+/-0.47 ml/kg/min and a plasma half-life of 6.8+/-0.8 min. Hepatic extraction was not detected. Valine-pyrrolidide addition did not affect extraction ratios significantly, but decreased (p=0.003) MCR to 4.18+/-0.27 ml/kg/min and increased (p=0.052) plasma half-life to 9.9+/-0.8 min. The metabolite was eliminated with a half-life of 22.1+/-2.6 min and a clearance of 2.07+/-0.11 ml/kg/min. In conclusion, intact GLP-2 is eliminated in the peripheral tissues, the splanchnic bed and the kidneys, but not in the liver, by mechanisms unrelated to DPP-IV. However, DPP-IV is involved in the overall GLP-2 metabolism and seems to be the sole enzyme responsible for N-terminal degradation of GLP-2.",
author = "Lene Hansen and Hare, {Kristine J} and Bolette Hartmann and Deacon, {Carolyn F} and Ugleholdt, {Randi K} and Astrid Plamboeck and Holst, {Jens J}",
note = "Keywords: Animals; Antigens, CD26; Glucagon-Like Peptide 2; Half-Life; Infusions, Intravenous; Kidney; Metabolic Clearance Rate; Pyrroles; Swine; Tissue Distribution; Valine",
year = "2006",
doi = "10.1016/j.regpep.2006.08.012",
language = "English",
volume = "138",
pages = "126--32",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "2-3",

}

RIS

TY - JOUR

T1 - Metabolism of glucagon-like peptide-2 in pigs: role of dipeptidyl peptidase IV.

AU - Hansen, Lene

AU - Hare, Kristine J

AU - Hartmann, Bolette

AU - Deacon, Carolyn F

AU - Ugleholdt, Randi K

AU - Plamboeck, Astrid

AU - Holst, Jens J

N1 - Keywords: Animals; Antigens, CD26; Glucagon-Like Peptide 2; Half-Life; Infusions, Intravenous; Kidney; Metabolic Clearance Rate; Pyrroles; Swine; Tissue Distribution; Valine

PY - 2006

Y1 - 2006

N2 - Little is known about the metabolism of the intestinotropic factor glucagon-like peptide-2 (GLP-2); except that it is a substrate for dipeptidyl peptidase IV (DPP-IV) and that it appears to be eliminated by the kidneys. We, therefore, investigated GLP-2 metabolism in six multicatheterized pigs receiving intravenous GLP-2 infusions (2 pmol/kg/min) before and after administration of valine-pyrrolidide (300 mumol/kg; a well characterized DPP-IV inhibitor). Plasma samples were analyzed by radioimmunoassays allowing determination of intact, biologically active GLP-2 and the DPP-IV metabolite GLP-2 (3-33). During infusion of GLP-2 alone, 30.9+/-1.7% of the infused peptide was degraded to GLP-2 (3-33). After valine-pyrrolidide, there was no significant formation of the metabolite. Significant extraction of intact GLP-2 was observed across the kidneys, the extremities (represented by a leg), and the splanchnic bed, resulting in a metabolic clearance rate (MCR) of 6.80+/-0.47 ml/kg/min and a plasma half-life of 6.8+/-0.8 min. Hepatic extraction was not detected. Valine-pyrrolidide addition did not affect extraction ratios significantly, but decreased (p=0.003) MCR to 4.18+/-0.27 ml/kg/min and increased (p=0.052) plasma half-life to 9.9+/-0.8 min. The metabolite was eliminated with a half-life of 22.1+/-2.6 min and a clearance of 2.07+/-0.11 ml/kg/min. In conclusion, intact GLP-2 is eliminated in the peripheral tissues, the splanchnic bed and the kidneys, but not in the liver, by mechanisms unrelated to DPP-IV. However, DPP-IV is involved in the overall GLP-2 metabolism and seems to be the sole enzyme responsible for N-terminal degradation of GLP-2.

AB - Little is known about the metabolism of the intestinotropic factor glucagon-like peptide-2 (GLP-2); except that it is a substrate for dipeptidyl peptidase IV (DPP-IV) and that it appears to be eliminated by the kidneys. We, therefore, investigated GLP-2 metabolism in six multicatheterized pigs receiving intravenous GLP-2 infusions (2 pmol/kg/min) before and after administration of valine-pyrrolidide (300 mumol/kg; a well characterized DPP-IV inhibitor). Plasma samples were analyzed by radioimmunoassays allowing determination of intact, biologically active GLP-2 and the DPP-IV metabolite GLP-2 (3-33). During infusion of GLP-2 alone, 30.9+/-1.7% of the infused peptide was degraded to GLP-2 (3-33). After valine-pyrrolidide, there was no significant formation of the metabolite. Significant extraction of intact GLP-2 was observed across the kidneys, the extremities (represented by a leg), and the splanchnic bed, resulting in a metabolic clearance rate (MCR) of 6.80+/-0.47 ml/kg/min and a plasma half-life of 6.8+/-0.8 min. Hepatic extraction was not detected. Valine-pyrrolidide addition did not affect extraction ratios significantly, but decreased (p=0.003) MCR to 4.18+/-0.27 ml/kg/min and increased (p=0.052) plasma half-life to 9.9+/-0.8 min. The metabolite was eliminated with a half-life of 22.1+/-2.6 min and a clearance of 2.07+/-0.11 ml/kg/min. In conclusion, intact GLP-2 is eliminated in the peripheral tissues, the splanchnic bed and the kidneys, but not in the liver, by mechanisms unrelated to DPP-IV. However, DPP-IV is involved in the overall GLP-2 metabolism and seems to be the sole enzyme responsible for N-terminal degradation of GLP-2.

U2 - 10.1016/j.regpep.2006.08.012

DO - 10.1016/j.regpep.2006.08.012

M3 - Journal article

C2 - 17107718

VL - 138

SP - 126

EP - 132

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 2-3

ER -

ID: 8416987