Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion

Research output: Contribution to journalJournal articleResearchpeer-review

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Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion. / Indo, Hiroko P.; Matsui, Hirofumi; Chen, Jing; Zhu, Haining; Hawkins, Clare Louise; Davies, Michael J.; Yarana, Chontida; Clair, Daret K St; Majima, Hideyuki J.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 57, No. 1, 01.07.2015, p. 13-20.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Indo, HP, Matsui, H, Chen, J, Zhu, H, Hawkins, CL, Davies, MJ, Yarana, C, Clair, DKS & Majima, HJ 2015, 'Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion', Journal of Clinical Biochemistry and Nutrition, vol. 57, no. 1, pp. 13-20. https://doi.org/10.3164/jcbn.14-146

APA

Indo, H. P., Matsui, H., Chen, J., Zhu, H., Hawkins, C. L., Davies, M. J., ... Majima, H. J. (2015). Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion. Journal of Clinical Biochemistry and Nutrition, 57(1), 13-20. https://doi.org/10.3164/jcbn.14-146

Vancouver

Indo HP, Matsui H, Chen J, Zhu H, Hawkins CL, Davies MJ et al. Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion. Journal of Clinical Biochemistry and Nutrition. 2015 Jul 1;57(1):13-20. https://doi.org/10.3164/jcbn.14-146

Author

Indo, Hiroko P. ; Matsui, Hirofumi ; Chen, Jing ; Zhu, Haining ; Hawkins, Clare Louise ; Davies, Michael J. ; Yarana, Chontida ; Clair, Daret K St ; Majima, Hideyuki J. / Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion. In: Journal of Clinical Biochemistry and Nutrition. 2015 ; Vol. 57, No. 1. pp. 13-20.

Bibtex

@article{13b5afd878744e0f88e2899aff80f74f,
title = "Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion",
abstract = "It hasbeendemonstratedthatcancercells areunderhigh levels of oxidative stress and express high levels of Manganese superoxide dismutase (MnSOD) to protect themselves and support the anabolic metabolism needed for growth and cell motility. The aim of this study was to identify proteins that may have a correlation with invasion and redox regulation by mitochondrial reactive oxygen species (ROS). MnSOD scavenges superoxide anions generated from mitochondria and is an important regulator of cellular redox status. Oxidative posttranslational modification of cysteine residues is a key mechanism that regulates protein structure and function. We hypothesized that MnSOD regulates intracellular reduced thiol status and promotes cancer invasion. A proteomic thiol-labeling approach with 5-iodoacetamidofluores-cein was used to identify changes in intracellular reduced thiol-containing proteins. Our results demonstrate that overexpression of MnSOD maintained the major structural protein, actin, in a reduced state, and enhanced the invasion ability in gastric mucosal cancer cells, RGK1. We also found that the expression of Talin and S100A4 were increased in MnSOD-overexpressed RGK1 cells. Moreover, Talin bound not only with actin but also with S100A4, suggesting that the interaction of these proteins may, in part, contribute to the invasive ability of rat gastric cancer.",
keywords = "Actin binding protein, Cancer invasion, MnSOD, Reduced form of actin, ROS",
author = "Indo, {Hiroko P.} and Hirofumi Matsui and Jing Chen and Haining Zhu and Hawkins, {Clare Louise} and Davies, {Michael J.} and Chontida Yarana and Clair, {Daret K St} and Majima, {Hideyuki J.}",
year = "2015",
month = "7",
day = "1",
doi = "10.3164/jcbn.14-146",
language = "English",
volume = "57",
pages = "13--20",
journal = "Journal of Clinical Biochemistry and Nutrition",
issn = "0912-0009",
publisher = "Institute of Applied Biochemistry",
number = "1",

}

RIS

TY - JOUR

T1 - Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin and enhances rat gastric tumor cell invasion

AU - Indo, Hiroko P.

AU - Matsui, Hirofumi

AU - Chen, Jing

AU - Zhu, Haining

AU - Hawkins, Clare Louise

AU - Davies, Michael J.

AU - Yarana, Chontida

AU - Clair, Daret K St

AU - Majima, Hideyuki J.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - It hasbeendemonstratedthatcancercells areunderhigh levels of oxidative stress and express high levels of Manganese superoxide dismutase (MnSOD) to protect themselves and support the anabolic metabolism needed for growth and cell motility. The aim of this study was to identify proteins that may have a correlation with invasion and redox regulation by mitochondrial reactive oxygen species (ROS). MnSOD scavenges superoxide anions generated from mitochondria and is an important regulator of cellular redox status. Oxidative posttranslational modification of cysteine residues is a key mechanism that regulates protein structure and function. We hypothesized that MnSOD regulates intracellular reduced thiol status and promotes cancer invasion. A proteomic thiol-labeling approach with 5-iodoacetamidofluores-cein was used to identify changes in intracellular reduced thiol-containing proteins. Our results demonstrate that overexpression of MnSOD maintained the major structural protein, actin, in a reduced state, and enhanced the invasion ability in gastric mucosal cancer cells, RGK1. We also found that the expression of Talin and S100A4 were increased in MnSOD-overexpressed RGK1 cells. Moreover, Talin bound not only with actin but also with S100A4, suggesting that the interaction of these proteins may, in part, contribute to the invasive ability of rat gastric cancer.

AB - It hasbeendemonstratedthatcancercells areunderhigh levels of oxidative stress and express high levels of Manganese superoxide dismutase (MnSOD) to protect themselves and support the anabolic metabolism needed for growth and cell motility. The aim of this study was to identify proteins that may have a correlation with invasion and redox regulation by mitochondrial reactive oxygen species (ROS). MnSOD scavenges superoxide anions generated from mitochondria and is an important regulator of cellular redox status. Oxidative posttranslational modification of cysteine residues is a key mechanism that regulates protein structure and function. We hypothesized that MnSOD regulates intracellular reduced thiol status and promotes cancer invasion. A proteomic thiol-labeling approach with 5-iodoacetamidofluores-cein was used to identify changes in intracellular reduced thiol-containing proteins. Our results demonstrate that overexpression of MnSOD maintained the major structural protein, actin, in a reduced state, and enhanced the invasion ability in gastric mucosal cancer cells, RGK1. We also found that the expression of Talin and S100A4 were increased in MnSOD-overexpressed RGK1 cells. Moreover, Talin bound not only with actin but also with S100A4, suggesting that the interaction of these proteins may, in part, contribute to the invasive ability of rat gastric cancer.

KW - Actin binding protein

KW - Cancer invasion

KW - MnSOD

KW - Reduced form of actin

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=84938348910&partnerID=8YFLogxK

U2 - 10.3164/jcbn.14-146

DO - 10.3164/jcbn.14-146

M3 - Journal article

C2 - 26236095

AN - SCOPUS:84938348910

VL - 57

SP - 13

EP - 20

JO - Journal of Clinical Biochemistry and Nutrition

JF - Journal of Clinical Biochemistry and Nutrition

SN - 0912-0009

IS - 1

ER -

ID: 152247521