Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

Research output: Contribution to journalJournal articleResearchpeer-review


  • Fulltext

    Final published version, 2.58 MB, PDF document

  • Anabela Bensimon-Brito
  • Giulia L M Boezio
  • João Cardeira-da-Silva
  • Astrid Wietelmann
  • Srinath Ramkumar
  • Lundegaard, Pia Rengtved
  • Christian S M Helker
  • Radhan Ramadass
  • Janett Piesker
  • Arno Nauerth
  • Clemens Mueller
  • Didier Y R Stainier

AIMS: Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque.

METHODS AND RESULTS: Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyze cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between hemodynamic defects and structural alterations of the heart, as observed clinically.

CONCLUSION: We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.

TRANSLATIONAL PERSPECTIVE: This study further illustrates the importance of the zebrafish model to investigate cardiovascular phenotypes including morphological and functional alterations as observed in human disease settings.

Original languageEnglish
JournalCardiovascular Research
Issue number12
Pages (from-to)2665–2687
Publication statusPublished - 2022

Bibliographical note

© The Author(s) 2021. Published by Oxford University Press on behalf of the European Society of Cardiology.

ID: 285874327