Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes

Research output: Contribution to journal › Journal article › peer-review

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Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes. / Larsen, S; Stride, N; Hey-Mogensen, Martin; Hansen, C N; Andersen, J L; Madsbad, S; Worm, D; Helge, J W; Dela, F.

In: Diabetologia, Vol. 54, No. 6, 2011, p. 1427-36.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Larsen, S, Stride, N, Hey-Mogensen, M, Hansen, CN, Andersen, JL, Madsbad, S, Worm, D, Helge, JW & Dela, F 2011, 'Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes', Diabetologia, vol. 54, no. 6, pp. 1427-36. https://doi.org/10.1007/s00125-011-2098-4

APA

Larsen, S., Stride, N., Hey-Mogensen, M., Hansen, C. N., Andersen, J. L., Madsbad, S., Worm, D., Helge, J. W., & Dela, F. (2011). Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes. Diabetologia, 54(6), 1427-36. https://doi.org/10.1007/s00125-011-2098-4

Vancouver

Larsen S, Stride N, Hey-Mogensen M, Hansen CN, Andersen JL, Madsbad S et al. Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes. Diabetologia. 2011;54(6):1427-36. https://doi.org/10.1007/s00125-011-2098-4

Author

Larsen, S ; Stride, N ; Hey-Mogensen, Martin ; Hansen, C N ; Andersen, J L ; Madsbad, S ; Worm, D ; Helge, J W ; Dela, F. / Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes. In: Diabetologia. 2011 ; Vol. 54, No. 6. pp. 1427-36.

Bibtex

@article{1050a5c0900e44bea3d685a5ac494e43,
title = "Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes",
abstract = "AIMS/HYPOTHESIS: Mitochondrial respiration has been linked to insulin resistance. We studied mitochondrial respiratory capacity and substrate sensitivity in patients with type 2 diabetes (patients), and obese and lean control participants.METHODS: Mitochondrial respiration was measured in permeabilised muscle fibres by respirometry. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl-carnitine). Myosin heavy chain (MHC) composition, antioxidant capacity (manganese superoxide dismutase [MnSOD]), citrate synthase activity and maximal oxygen uptake (VO2) were also determined. Insulin sensitivity was determined with the isoglycaemic-hyperinsulinaemic clamp technique.RESULTS: Insulin sensitivity was different (p < 0.05) between the groups (patientsCONCLUSIONS/INTERPRETATION: Increased mitochondrial substrate sensitivity is seen in skeletal muscle from type 2 diabetic patients and is confined to non-lipid substrates. Respiratory capacity per mitochondrion is not decreased with insulin resistance.",
author = "S Larsen and N Stride and Martin Hey-Mogensen and Hansen, {C N} and Andersen, {J L} and S Madsbad and D Worm and Helge, {J W} and F Dela",
year = "2011",
doi = "10.1007/s00125-011-2098-4",
language = "English",
volume = "54",
pages = "1427--36",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes

AU - Larsen, S

AU - Stride, N

AU - Hey-Mogensen, Martin

AU - Hansen, C N

AU - Andersen, J L

AU - Madsbad, S

AU - Worm, D

AU - Helge, J W

AU - Dela, F

PY - 2011

Y1 - 2011

N2 - AIMS/HYPOTHESIS: Mitochondrial respiration has been linked to insulin resistance. We studied mitochondrial respiratory capacity and substrate sensitivity in patients with type 2 diabetes (patients), and obese and lean control participants.METHODS: Mitochondrial respiration was measured in permeabilised muscle fibres by respirometry. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl-carnitine). Myosin heavy chain (MHC) composition, antioxidant capacity (manganese superoxide dismutase [MnSOD]), citrate synthase activity and maximal oxygen uptake (VO2) were also determined. Insulin sensitivity was determined with the isoglycaemic-hyperinsulinaemic clamp technique.RESULTS: Insulin sensitivity was different (p < 0.05) between the groups (patientsCONCLUSIONS/INTERPRETATION: Increased mitochondrial substrate sensitivity is seen in skeletal muscle from type 2 diabetic patients and is confined to non-lipid substrates. Respiratory capacity per mitochondrion is not decreased with insulin resistance.

AB - AIMS/HYPOTHESIS: Mitochondrial respiration has been linked to insulin resistance. We studied mitochondrial respiratory capacity and substrate sensitivity in patients with type 2 diabetes (patients), and obese and lean control participants.METHODS: Mitochondrial respiration was measured in permeabilised muscle fibres by respirometry. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl-carnitine). Myosin heavy chain (MHC) composition, antioxidant capacity (manganese superoxide dismutase [MnSOD]), citrate synthase activity and maximal oxygen uptake (VO2) were also determined. Insulin sensitivity was determined with the isoglycaemic-hyperinsulinaemic clamp technique.RESULTS: Insulin sensitivity was different (p < 0.05) between the groups (patientsCONCLUSIONS/INTERPRETATION: Increased mitochondrial substrate sensitivity is seen in skeletal muscle from type 2 diabetic patients and is confined to non-lipid substrates. Respiratory capacity per mitochondrion is not decreased with insulin resistance.

U2 - 10.1007/s00125-011-2098-4

DO - 10.1007/s00125-011-2098-4

M3 - Journal article

C2 - 21424396

VL - 54

SP - 1427

EP - 1436

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 6

ER -

ID: 33837560