Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation. / Bonzon-Kulichenko, Elena; Camafeita, Emilio; Antonio Lopez, Juan; Gomez-Serrano, Maria; Jorge, Inmaculada; Calvo, Enrique; Nunez, Estefania; Trevisan-Herraz, Marco; Bagwan, Navratan; Antonio Barcena, Jose; Peral, Belen; Vazquez, Jesus.

In: Journal of Proteomics, Vol. 214, 103624, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bonzon-Kulichenko, E, Camafeita, E, Antonio Lopez, J, Gomez-Serrano, M, Jorge, I, Calvo, E, Nunez, E, Trevisan-Herraz, M, Bagwan, N, Antonio Barcena, J, Peral, B & Vazquez, J 2020, 'Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation', Journal of Proteomics, vol. 214, 103624. https://doi.org/10.1016/j.jprot.2019.103624

APA

Bonzon-Kulichenko, E., Camafeita, E., Antonio Lopez, J., Gomez-Serrano, M., Jorge, I., Calvo, E., Nunez, E., Trevisan-Herraz, M., Bagwan, N., Antonio Barcena, J., Peral, B., & Vazquez, J. (2020). Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation. Journal of Proteomics, 214, [103624]. https://doi.org/10.1016/j.jprot.2019.103624

Vancouver

Bonzon-Kulichenko E, Camafeita E, Antonio Lopez J, Gomez-Serrano M, Jorge I, Calvo E et al. Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation. Journal of Proteomics. 2020;214. 103624. https://doi.org/10.1016/j.jprot.2019.103624

Author

Bonzon-Kulichenko, Elena ; Camafeita, Emilio ; Antonio Lopez, Juan ; Gomez-Serrano, Maria ; Jorge, Inmaculada ; Calvo, Enrique ; Nunez, Estefania ; Trevisan-Herraz, Marco ; Bagwan, Navratan ; Antonio Barcena, Jose ; Peral, Belen ; Vazquez, Jesus. / Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation. In: Journal of Proteomics. 2020 ; Vol. 214.

Bibtex

@article{f2fc430f57f14f94bc6e23bcc8dfabe5,
title = "Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation",
abstract = "Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.",
keywords = "Thiol redox proteome, Filter-aided sample preparation, FASILOX, Cys oxidation, Adipose tissue, Sexual dimorphism",
author = "Elena Bonzon-Kulichenko and Emilio Camafeita and {Antonio Lopez}, Juan and Maria Gomez-Serrano and Inmaculada Jorge and Enrique Calvo and Estefania Nunez and Marco Trevisan-Herraz and Navratan Bagwan and {Antonio Barcena}, Jose and Belen Peral and Jesus Vazquez",
year = "2020",
doi = "10.1016/j.jprot.2019.103624",
language = "English",
volume = "214",
journal = "Journal of Proteomics",
issn = "1874-3919",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation

AU - Bonzon-Kulichenko, Elena

AU - Camafeita, Emilio

AU - Antonio Lopez, Juan

AU - Gomez-Serrano, Maria

AU - Jorge, Inmaculada

AU - Calvo, Enrique

AU - Nunez, Estefania

AU - Trevisan-Herraz, Marco

AU - Bagwan, Navratan

AU - Antonio Barcena, Jose

AU - Peral, Belen

AU - Vazquez, Jesus

PY - 2020

Y1 - 2020

N2 - Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.

AB - Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.

KW - Thiol redox proteome

KW - Filter-aided sample preparation

KW - FASILOX

KW - Cys oxidation

KW - Adipose tissue

KW - Sexual dimorphism

U2 - 10.1016/j.jprot.2019.103624

DO - 10.1016/j.jprot.2019.103624

M3 - Journal article

C2 - 31874222

VL - 214

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

M1 - 103624

ER -

ID: 237418814