Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Research output: Contribution to journal › Journal article › peer-review

Standard

Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo. / Murphy, Michael P.; Bayir, Hülya; Belousov, Vsevolod; Chang, Christopher J.; Davies, Kelvin J.A.; Davies, Michael J.; Dick, Tobias P.; Finkel, Toren; Forman, Henry J.; Janssen-Heininger, Yvonne; Gems, David; Kagan, Valerian E.; Kalyanaraman, Balaraman; Larsson, Nils Göran; Milne, Ginger L.; Nyström, Thomas; Poulsen, Henrik E.; Radi, Rafael; Van Remmen, Holly; Schumacker, Paul T.; Thornalley, Paul J.; Toyokuni, Shinya; Winterbourn, Christine C.; Yin, Huiyong; Halliwell, Barry.

In: Nature Metabolism, Vol. 4, No. 6, 2022, p. 651-662.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Murphy, MP, Bayir, H, Belousov, V, Chang, CJ, Davies, KJA, Davies, MJ, Dick, TP, Finkel, T, Forman, HJ, Janssen-Heininger, Y, Gems, D, Kagan, VE, Kalyanaraman, B, Larsson, NG, Milne, GL, Nyström, T, Poulsen, HE, Radi, R, Van Remmen, H, Schumacker, PT, Thornalley, PJ, Toyokuni, S, Winterbourn, CC, Yin, H & Halliwell, B 2022, 'Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo', Nature Metabolism, vol. 4, no. 6, pp. 651-662. https://doi.org/10.1038/s42255-022-00591-z

APA

Murphy, M. P., Bayir, H., Belousov, V., Chang, C. J., Davies, K. J. A., Davies, M. J., Dick, T. P., Finkel, T., Forman, H. J., Janssen-Heininger, Y., Gems, D., Kagan, V. E., Kalyanaraman, B., Larsson, N. G., Milne, G. L., Nyström, T., Poulsen, H. E., Radi, R., Van Remmen, H., ... Halliwell, B. (2022). Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo. Nature Metabolism, 4(6), 651-662. https://doi.org/10.1038/s42255-022-00591-z

Vancouver

Murphy MP, Bayir H, Belousov V, Chang CJ, Davies KJA, Davies MJ et al. Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo. Nature Metabolism. 2022;4(6):651-662. https://doi.org/10.1038/s42255-022-00591-z

Author

Murphy, Michael P. ; Bayir, Hülya ; Belousov, Vsevolod ; Chang, Christopher J. ; Davies, Kelvin J.A. ; Davies, Michael J. ; Dick, Tobias P. ; Finkel, Toren ; Forman, Henry J. ; Janssen-Heininger, Yvonne ; Gems, David ; Kagan, Valerian E. ; Kalyanaraman, Balaraman ; Larsson, Nils Göran ; Milne, Ginger L. ; Nyström, Thomas ; Poulsen, Henrik E. ; Radi, Rafael ; Van Remmen, Holly ; Schumacker, Paul T. ; Thornalley, Paul J. ; Toyokuni, Shinya ; Winterbourn, Christine C. ; Yin, Huiyong ; Halliwell, Barry. / Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo. In: Nature Metabolism. 2022 ; Vol. 4, No. 6. pp. 651-662.

Bibtex

@article{1c3bb954a1ae49209dffec0993adbbc0,
title = "Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo",
abstract = "Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.",
author = "Murphy, {Michael P.} and H{\"u}lya Bayir and Vsevolod Belousov and Chang, {Christopher J.} and Davies, {Kelvin J.A.} and Davies, {Michael J.} and Dick, {Tobias P.} and Toren Finkel and Forman, {Henry J.} and Yvonne Janssen-Heininger and David Gems and Kagan, {Valerian E.} and Balaraman Kalyanaraman and Larsson, {Nils G{\"o}ran} and Milne, {Ginger L.} and Thomas Nystr{\"o}m and Poulsen, {Henrik E.} and Rafael Radi and {Van Remmen}, Holly and Schumacker, {Paul T.} and Thornalley, {Paul J.} and Shinya Toyokuni and Winterbourn, {Christine C.} and Huiyong Yin and Barry Halliwell",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2022",
doi = "10.1038/s42255-022-00591-z",
language = "English",
volume = "4",
pages = "651--662",
journal = "Nature Metabolism",
issn = "2522-5812",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

AU - Murphy, Michael P.

AU - Bayir, Hülya

AU - Belousov, Vsevolod

AU - Chang, Christopher J.

AU - Davies, Kelvin J.A.

AU - Davies, Michael J.

AU - Dick, Tobias P.

AU - Finkel, Toren

AU - Forman, Henry J.

AU - Janssen-Heininger, Yvonne

AU - Gems, David

AU - Kagan, Valerian E.

AU - Kalyanaraman, Balaraman

AU - Larsson, Nils Göran

AU - Milne, Ginger L.

AU - Nyström, Thomas

AU - Poulsen, Henrik E.

AU - Radi, Rafael

AU - Van Remmen, Holly

AU - Schumacker, Paul T.

AU - Thornalley, Paul J.

AU - Toyokuni, Shinya

AU - Winterbourn, Christine C.

AU - Yin, Huiyong

AU - Halliwell, Barry

N1 - Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer Nature Limited.

PY - 2022

Y1 - 2022

N2 - Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.

AB - Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.

UR - http://www.scopus.com/inward/record.url?scp=85132951828&partnerID=8YFLogxK

U2 - 10.1038/s42255-022-00591-z

DO - 10.1038/s42255-022-00591-z

M3 - Journal article

C2 - 35760871

AN - SCOPUS:85132951828

VL - 4

SP - 651

EP - 662

JO - Nature Metabolism

JF - Nature Metabolism

SN - 2522-5812

IS - 6

ER -

ID: 313874212